Cardiac cachexia -pathophysiology and its mechanisms-

心脏恶病质-病理生理学及其机制-

• 批准号: 07457297
• 负责人: IMOTO Kiyotaka
• 金额: $ 4.61万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457297/
• 关键词: cardiac cachexia; insulin resistance; glucose transporter; 心臓悪胞質; グルコースクランプ; グルコーストランスポーター

1) Insulin resistance in cardiac patientsEuglycemic hyperinsulinemic glucose clamp was performed in 14 cardiovascular patients, 7 with valvular disease, 3 with arteriosclerotic disease and 4 with is ischmic heart disease and compared with nomal volunteer. Metabolic rate of insulin (MCRi) in cardiac patients were not significantly different from that of control, 464.3(]SY.+-。[)92.8vs. 419.1(]SY.+-。[)60.7ml. min. m<@D12@>D1. M value or glucose metabolized in cardiac patients was significantley lower than that of control, 5.34(]SY.+-。[)1.80 and 8.56(]SY.+-。[)0.75 mg/kg/min, respectively. Those were not dependent upon the difference in disease.2) Intracellular glucose metabolismREE of the control was 1265+127 kcal was not different from 1270+150kcal of patients. REE/BMR was correlated with M value with r=0.63 and p<0.05. Although glucose oxidation was not different, glucose storage was significant ly decreased in patient group, 2.31(]SY.+-。[)1.37 and 4.94(]SY.+-。[)0.43 mg/kg/min, respectiv … More ely. Glucose oxidation and M value were strongly correalted with r=0.88 and p<0.001.3) Glycogen contents in the myocardiumWe have established the method to measure tissue glycogen contents from a small amount of biopsied samples. In the cancer patients, glycogen contents in the skeletam muscle was positively correlated with M value obtained from clamp study, more strongly with glucose storage. In the cardiac patients, those strong correlation was not observed (currently reexamied). Energy substrates, ATP,ADP and AMP,were measured by HPLC and now under analysis.4) Relation with IL-6IL-6 in the peripheral blood was measured by ELIZA.Significant elevation of IL-6 was observed in only 2 out of 14 patients. Those were not the patients with decreased M values.5) Glucose transporters in the cardiac muscleImmunohistochmically glucose transporter4 was documented in the human cardiac muscle. We constructed primers of Glut4 and examined the presence of mRNA of Glut4 in the cardiac muscle. Recently we clearly documented the presence of Glut4 mRNA in the cardiac muscle and are now continuously investigating the quantity by competitive RT-PCR. Less

1)心脏病患者的胰岛素抵抗对14名心血管患者进行正常血糖高胰岛素葡萄糖钳夹试验，其中7名患有瓣膜疾病，3名患有动脉硬化疾病，4名患有缺血性心脏病，并与正常志愿者进行比较。心脏病患者的胰岛素(MCRi)代谢率与对照组无显着差异，464.3(]SY.+-。[)92.8vs. 419.1(]SY.+-。[)60.7ml。分钟。 m<@D12@>D1。心脏病患者的M值或葡萄糖代谢显着低于对照组，分别为5.34(]SY.+-。[)1.80和8.56(]SY.+-。[)0.75 mg/kg/min。这些不依赖于疾病的差异。2)对照的细胞内葡萄糖代谢REE为1265+127kcal，与患者的1270+150kcal没有差异。 REE/BMR 与 M 值相关，r=0.63，p<0.05。尽管葡萄糖氧化没有差异，但患者组的葡萄糖储存显着减少，分别为 2.31(]SY.+-。[)1.37 和 4.94(]SY.+-。[)0.43 mg/kg/min。葡萄糖氧化和M值与r=0.88和p<0.001.3强烈相关。3）心肌中的糖原含量我们建立了从少量活检样品中测量组织糖原含量的方法。在癌症患者中，骨骼肌中的糖原含量与钳夹研究获得的M值呈正相关，与葡萄糖储存呈更强烈的相关。在心脏病患者中，没有观察到这些强相关性（目前正在复查）。通过HPLC测量能量底物ATP、ADP和AMP，目前正在分析中。4)通过ELIZA测量外周血中IL-6与IL-6的关系。14名患者中仅2名观察到IL-6显着升高。这些患者并非 M 值降低的患者。5) 心肌中的葡萄糖转运蛋白 免疫组织化学上记录了人类心肌中的葡萄糖转运蛋白 4。我们构建了 Glut4 的引物并检查了心肌中 Glut4 mRNA 的存在。最近，我们清楚地记录了心肌中 Glut4 mRNA 的存在，现在正在通过竞争性 RT-PCR 不断研究其数量。较少的

项目成果

Yoshikawa T: "Alimentary hyperglucemia in patients with subtotal gastrectomy is associated with clecreased insulin secretion but not with insulin resistance" International Surgery. 81. 32-35 (1996)

Yoshikawa T：“胃次全切除术患者的消化性高血糖与胰岛素分泌减少有关，但与胰岛素抵抗无关”《国际外科》。

Yoshikawa T: "Alimentary hyperglucemia in patients with subtotal gastrectomy is associated with decreased insulin secretion but not with insulin resistance" International Surgery. 81. 32-35 (1996)

Yoshikawa T：“胃次全切除术患者的消化性高血糖与胰岛素分泌减少有关，但与胰岛素抵抗无关”国际外科。

4) Sato Y.: "Immunohistochmical localization of facilitated diffusion glucose transporters in rat pancreatic islet." Tissue and Cell. 28. 637-643 (1996)

4) Sato Y.：“大鼠胰岛中促进扩散葡萄糖转运蛋白的免疫组织化学定位。”

Sato Y: "Immunohistochemical localozation of facilitated-diffusion glucose transporters in rat pancreatic islet." Tissue & Cell. 28. 637-643 (1996)

Sato Y：“大鼠胰岛中易化扩散葡萄糖转运蛋白的免疫组织化学定位。”

Noguchi, Y.: "Are cytokines possible mediators of cancer cachexia?" Surgery Today. 26. 467-475 (1996)

Noguchi, Y.：“细胞因子可能是癌症恶病质的介质吗？”