Migration and Morphological Change of Wounded Endothelial Cells, and Effects of Flow

1. 刮取融合培养的内皮细胞，用视频显微镜观察边缘。随机游走，每个细胞的迁移量为224<正负>153mum/24h（平均<正负> sd）。在零钙培养基中培养24小时后，上皮细胞部分分离。迁移距离为111<正负bb0 79mum/24h，小于对照试验，无统计学意义3。创面周围内皮细胞的ICAM-1和VCAM-1免疫组化染色均为阴性。研究了胶原凝胶与人体组织共培养系统对损伤内皮细胞增殖的促进作用。将牛主动脉内皮细胞播种于与人脂肪组织、横纹肌、网膜和静脉混合的胶原凝胶上。人网膜和横纹肌增强内皮细胞复制。应用内皮细胞和成纤维细胞种子，建立了生物假体细胞相容性的体外检测系统。

1. Confluent cultured endothelial cells were scraped and the edge were observed by videomicroscope. migration of each cells were 224<plus-minus>153mum/24hours(mean<plus-minus>S.D.) with rndom walkEndothelial cells were detached partially after 24 hours incubation in zero calcium medium. Distance of migration was 111<plus-minus>79mum/24hours and less than control experiment without statically significanc3. Imunohistochemical staining of ICAM-1 and VCAM-1 was negative on endothelial cells around the wound edge.4. Enhacement of wounded endothalial cells reproduction was examined in collagen gel co-culture system with human tissue. Bovine aortic endothelial cells was seeded on collagen gel mixed with human adipose tisuue, striated muscle, omentum, and vein. Human omentum and striated muscle enhanced endothelial cell replication.5. In vitro system to examine cell compatibility of bioprosthesis was developd with applicatin of endothelial and fibroblast seeding.