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Cellbiological study of tooth resorption and repair.

牙齿吸收和修复的细胞生物学研究。

基本信息

批准号：
07457450
负责人：
SAHARA Noriyuki
金额：
$ 4.1万
依托单位：
Mastumoto Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457450/
关键词：
Human Deciduous Teeth Resorption Repair Odontoclast Cytokine Histochemistry Immunohistochemistry

项目摘要

In human deciduous teeth, internal resorption of the coronal dentin takes place prior to shedding, and this resorption shows clear time-related histological changes. Furthermore, this phenomenon is closely associated with inflammatory reaction in the pulp. To determine possible factors involved in tooth resorption, in this study, immunohistochemical distribution of inflammatory cytokines such as IL-1, IL-6 and TNF in the various stage during the internal resorption of deciduous teeth. During the time the roots were actively being resorbed, no cytokines-positive cells could be found in the pulp. However, when the root resorption neared completion, cytokines-positive mononuclear cells started to gradually infiltrate into the pulp. After that, odontoclasts appeared in the pulp, and the resorption proceeded from the predentin to the dentin, and the number of cytokines-positive cells in the pulp gradually increased. At the end of the internal resorption, odontoclasts became detached from the resorbed dentin surface, and the dentinal resorption lacunae were filled by deposifion of cementum-like tissue. Throughout the process of this internal resorption and repair, cytokines-positive cells were always found in closely proximity to odontoclasts. The present study demonstrates in vivo that inflammatory cytokines may directly and/or indirectly participate in tooth resorption and repair.

在人类乳牙中，冠状牙本质的内部吸收发生在脱落之前，并且这种吸收显示出明显的与时间相关的组织学变化。此外，这种现象与牙髓中的炎症反应密切相关。为探讨乳牙内吸收的可能影响因素，本研究采用免疫组织化学方法检测了乳牙内吸收各阶段炎症细胞因子IL-1、IL-6和TNF的分布。在这段时间的根积极被吸收，没有细胞因子阳性细胞可以在牙髓中找到。然而，当牙根吸收接近完成时，细胞因子阳性单核细胞开始逐渐浸润到牙髓中。在此之后，破牙本质细胞出现在牙髓中，并且吸收从前牙本质向牙本质进行，牙髓中的细胞因子阳性细胞数量逐渐增加。内吸收结束时，破牙本质细胞脱离吸收的牙本质表面，牙本质吸收陷窝被牙骨质样组织填充。在整个内吸收和修复的过程中，细胞因子阳性细胞总是被发现在破牙本质细胞附近。本研究表明，在体内，炎症细胞因子可能直接和/或间接参与牙齿吸收和修复。