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Study on the cooperative bactericidal activities between neutrophis and antibiotics, electrolyzed-oxidizing water in serious infection disease

中性粒细胞与抗生素、电解氧化水在严重感染性疾病中协同杀菌作用的研究

基本信息

批准号：
07457482
负责人：
KIMURA Hiroto
金额：
$ 2.18万
依托单位：
HIROSAKI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

O_2^<・->production abnormalities of neutrophils in peripheral blood with severely infectious disease in maxillofacial region were investigated by CLA dependent chemiluminescence method. The effect of antibiotics on O_2^<・->production of neutrophils was also studied. Furthermore, bactericidal effects of electrolyzed-oxidizing water on Stapylococcus aureus (S.aureus) were investigated by O_2^<・->production of neutrophils and expression of integrin molecules.Results were as follows.1. O_2^<・->production of neutrophils obtained from 12 patients with oral cancer and 6 patients with mandublar osteomyelitis showed lower values than heal thy volunteers. Six of 12 patients with oral cancer were suffered from postoperative infection by MRSA.2. Four antibiotics (ASPC,NTL,ISP,FLRX) increased O_2^<・->production of neutrophils to show cooperative bactericidal activities. And three of them (ASPC,NTL,FLRX) increased the expression of C3b recepter on the suface of neutrophil membrane.13EA04 : 3. O_2^<・->production of neutrophils accompanying with phagocytesis of S.aureaus (1*10^8/ml) , which were pretreated by electrolyzed-oxidizing water, showed 50% of bacteria was injured by this treatment. The expression of integrin molecules' (CD11b and CD18) on the surface of neutrophil membrane with phagocytosing S.aureus revealed that 70-80% of bacteria lost their biological activities by the treatment with electrolyzed-oxidizing water.4. Surgical wound infected by MRSA was remarkably improved by washing with electrolyzed-oxidizing water. In treating MRSA infection patients, optimal dosage of Vancomycin^<<encircledR>>was determined by monitoring its concentration in peripheral blood.These results suggested that it was useful to choose antibiotics based on the cooperative activity which increased O_2^<・->production of neutrophils. Electrolyzed-oxidizing water showed fatal effects on peccant bacteria, therefore, its clinical use was more recommended.

应用CLA依赖性化学发光法研究颌面部严重感染性疾病患者外周血中性粒细胞O_2^<·->生成异常。还研究了抗生素对中性粒细胞O_2^<·->生成的影响。此外，通过O_2^<··->中性粒细胞的产生和整合素分子的表达，研究了电解氧化水对金黄色葡萄球菌的杀菌作用。结果如下：12例口腔癌患者和6例下颌骨髓炎患者的中性粒细胞O_2^<·->的生成值低于正常对照组。12例口腔癌患者中有6例术后发生mrsa感染。4种抗生素（ASPC、NTL、ISP、FLRX）均能增加中性粒细胞O_2^<·->的产生，表现出协同杀菌活性。其中3种（ASPC、NTL、FLRX）增加了中性粒细胞膜表面C3b受体的表达。[00:04 . 3]经电解氧化水预处理的金黄色葡萄球菌的O_2^<·->中性粒细胞的产生（1*10^8/ml）表明，该处理对50%的细菌造成了损伤。整合素分子（CD11b和CD18）在吞噬金黄色葡萄球菌中性粒细胞膜表面的表达表明，经电解氧化水处理后，70-80%的细菌失去了生物活性。经氧化电解水冲洗后，MRSA感染的手术伤口有明显改善。在治疗MRSA感染患者时，通过监测万古霉素在外周血中的浓度来确定万古霉素^<<encircledR>>的最佳剂量。这些结果表明，根据协同活性来选择抗生素是有益的，可以增加中性粒细胞O_2^<·->的产生。电解氧化水对大肠杆菌有致死性作用，故更推荐临床使用。