Study on the cooperative bactericidal activities between neutrophis and antibiotics, electrolyzed-oxidizing water in serious infection disease

production abnormalities of neutrophils in peripheral blood with severely infectious diseasein maxillofacial region were investigated by CLA dependent chemiluminescence方法The effect ofantibiotics on o_2 production of neutrophils was also studied. Furthermore，bactericidal effects of electrolyzed-oxidizing water on Stapylococcus aureus wereinvestigated by o_2 production of neutrophils and expression of integrin molecules.Results wereas follows.1. o_2 production of neutrophils obtained from 12 patients with oral cancer and 6patients with mandublar osteomyelitis showed low values than heal thy volunteers. Six of 12patients with oral cancer were suffered from postoperative infection by MRSA.2. Four antibiotics（ASPC，NTL，ISP，FLRX） increased o_2 production of neutrophils to show cooperative bactericidalactivities. And three of them (ASPC，NTL，FLRX) increased the expression of C3b recepter on the sufaceneutrophil membrane. 13ea04：3. o_2 ^< ->production of neutrophils accompanying with phagocytesis of s.u reaus （1*10^8/ml）which were pretreated by electrolyzed-oxidizing watershowed 50% of bacteria was injured by this treatment. The表达式integrin molecules' (CD11band CD18) on the surface of neutrophil membrane with phagocytosing S.aureus revealed that 70-80% ofbacteria失落的他们的生物活动by the treatment with electrolyzed-oxidizing water.4.由MRSA进行的外科手术治疗报告用电子手术治疗water. In treating MRSA infection patients，optimal dosage of Vancomycin was determined by monitoring its concentration in <<encircledR>>was determined by monitoring its concentration inperipheral blood.These results suggested that was useful to choose antibiotics based on thecooperative activity which increased o_2 production of neutrophils. Electrolyzed-oxidizing watershowed fatal effects on peccant bacteria, therefore，其临床应用更多推荐。

O_2^<・->production abnormalities of neutrophils in peripheral blood with severely infectious disease in maxillofacial region were investigated by CLA dependent chemiluminescence method. The effect of antibiotics on O_2^<・->production of neutrophils was also studied. Furthermore, bactericidal effects of electrolyzed-oxidizing water on Stapylococcus aureus (S.aureus) were investigated by O_2^<・->production of neutrophils and expression of integrin molecules.Results were as follows.1. O_2^<・->production of neutrophils obtained from 12 patients with oral cancer and 6 patients with mandublar osteomyelitis showed lower values than heal thy volunteers. Six of 12 patients with oral cancer were suffered from postoperative infection by MRSA.2. Four antibiotics (ASPC,NTL,ISP,FLRX) increased O_2^<・->production of neutrophils to show cooperative bactericidal activities. And three of them (ASPC,NTL,FLRX) increased the expression of C3b recepter on the suface of neutrophil membrane.13EA04 : 3. O_2^<・->production of neutrophils accompanying with phagocytesis of S.aureaus (1*10^8/ml) , which were pretreated by electrolyzed-oxidizing water, showed 50% of bacteria was injured by this treatment. The expression of integrin molecules' (CD11b and CD18) on the surface of neutrophil membrane with phagocytosing S.aureus revealed that 70-80% of bacteria lost their biological activities by the treatment with electrolyzed-oxidizing water.4. Surgical wound infected by MRSA was remarkably improved by washing with electrolyzed-oxidizing water. In treating MRSA infection patients, optimal dosage of Vancomycin^<<encircledR>>was determined by monitoring its concentration in peripheral blood.These results suggested that it was useful to choose antibiotics based on the cooperative activity which increased O_2^<・->production of neutrophils. Electrolyzed-oxidizing water showed fatal effects on peccant bacteria, therefore, its clinical use was more recommended.