Molecular biological study on the pathophysiological substances and their interactions concerned with the enlargement of jaw cyst

颌骨囊肿增大病理生理物质及其相互作用的分子生物学研究

• 批准号: 10470426
• 负责人: KIMURA Hiroto
• 金额: $ 4.1万
• 依托单位: HIROSAKI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10470426/
• 关键词: jaw cyst; bone resortion; inflanunatory cytokine; cyst wall fibroblast; immunohistolocal stainin; messenger RNA; PCR; nitricoxide radical; 嚢胞壁細胞

A few researchers have suggested that the intracystic fluid pressure and/or several bone resorbing factors might concern with the jaw cyst enlargement. Therefore, in this study, the production of inflammatory cytokines and nitric oxide (NO) in cyst fluid and/or cyst wall were investigated because we speculated these pathophysiological substances might affect the functioh of osteoblasts and/or osteoclasts located in surrounding bone of jaw cyst. Results were as follows.1. Interleukin 6 (IL-6) and tumor necrosis factor alfa (TNF-α) were detected in the cyst wall tissue and it was confirmed that the fibroblast mainly produced both cytokines.2. Using RT-PCR analysis, the expressions of messenger RNA for inflammatory cytokines (IL-6, IL-8, TNF-α, IFN-γ, TGF-β1) and the recepter of cytokines and eicosanoids (IL-6R α, gp130, TNFR I, CXCR2) were detected in supernatants of cultured fibroblast from the radicular cyst wall.3. Interleukin beta (IL-β), transforming growth factor beta 1 (TGF- β 1) and macrophage colony stimulating factor (M-CSF) were detected in the cyst fluid. NO metabolites (NOィイD22ィエD2 /NOィイD23ィエD2) were detected in the cyst fluid obtained from radicular cysts and postoperative maxillary cysts.These results suggested that such pathophysiological substances as inflammatory cytokines, eicosanoids and nitric oxide metabolites were related to the mechanism of the resorption of surrounding bone adjacent to cyst wall. These studies also revealed that the production of bone resorbing factors above described played important roles in the enlargement of jaw cyst and that these factors were derived from fibroblasts in cyst wall and were probably induced by mechanical stress.

一些研究人员认为囊内流体压力和/或一些骨吸收因素可能与颌囊肿增大有关。因此，本研究对囊肿液和/或囊壁中炎性细胞因子和一氧化氮（NO）的产生进行了研究，因为我们推测这些病理生理物质可能影响颌骨囊肿周围骨中的成骨细胞和/或破骨细胞的功能。结果如下： 1．囊壁组织中检测到白细胞介素6（IL-6）和肿瘤坏死因子α（TNF-α），证实成纤维细胞主要产生这两种细胞因子。 2．采用 RT-PCR 分析，在培养的根囊成纤维细胞上清液中检测到炎症细胞因子（IL-6、IL-8、TNF-α、IFN-γ、TGF-β1）信使 RNA 以及细胞因子和类二十烷酸受体（IL-6R α、gp130、TNFR I、CXCR2）的表达 墙.3。囊液中检测到白细胞介素β（IL-β）、转化生长因子β1（TGF-β1）和巨噬细胞集落刺激因子（M-CSF）。根尖囊肿及术后上颌骨囊肿的囊肿液中检测到NO代谢物（NOィイD22ィエD2 /NOィイD23ィエD2）。这些结果提示炎性细胞因子、类二十烷酸和一氧化氮代谢物等病理生理物质与囊肿周围骨的吸收机制有关。 墙。这些研究还表明，上述骨吸收因子的产生在颌骨囊肿的扩大中起着重要作用，这些因子来源于囊肿壁的成纤维细胞，可能是由机械应力诱导的。

项目成果

Hiroto KIMURA: "Study on the inflammatory cytokines produced in jaw cyst walls"International Journal of Oral & Maxillofacial Surgery. 28・Suppl.. 35-35 (1999)

Hiroto KIMURA：“颌囊肿壁中产生的炎性细胞因子的研究”国际口腔颌面外科杂志 28·增刊 35-35 (1999)。

Kimura Hiroto: "Pathogenesis of jaw cystenlargement"The hirosaki Medical Journal. 51. 54-60 (1999)

木村博人：“颌骨囊肿增大的发病机制”弘前医学杂志。

Hiroto KIMURA: "Pathogenesis of jaw cyst enlargement"Hirosaki Medical Journal. 51・Suppl.. 54-60 (1999)

Hiroto KIMURA：“颌骨囊肿增大的发病机制”弘前医学杂志 51・增刊 54-60 (1999)