Molecular biological study on the pathophysiological substances and their interactions concerned with the enlargement of jaw cyst

A few researchers have suggested that the intracystic fluid pressure and/or several bone resorbing factors might concern with the jaw cyst enlargement。Therefore，in this study，the production of inflammatory cytokines and nitric oxide(NO)in cyst fluid and/or cyst wall were investigated because we speculated these pathophysiological substances might affect the functioh of osteoblasts and/or osteoclasts located in surrounding bone of jaw cyst。Results were as follows.1。Interleukin6(IL-6)and tumor necrosis factor alfa(TNF-α)were detected in the cyst wall tissue and it was confirmed that the fibroblast mainly produced both cytokines.2。Using RT-PCR analysis，the expressions of messenger RNA for inflammatory cytokines(IL-6，IL-8，TNF-α，IFN-γ，TGF-β1)and the recepter of cytokines and eicosanoids(IL-6Rα，gp130，TNFR I，CXCR2)were detected in supernatants of cultured fibroblast from the radicular cyst wall.3.Interleukin beta(IL-β)，transforming growth factor beta1(TGF-β1)and macrophage colony stimulating factor(M-CSF)were detected in the cyst fluid.NO metabolites(NO-D 22-D 2/NO-D 23-D2)were detected in the cyst fluid obtained from radicular cysts and postoperative maxillary cysts.These results suggested that such pathophysiological substances as inflammatory cytokines，eicosanoids and nitric oxide metabolites were related to the mechanism of the resorption of surrounding bone adjacent to cyst wall.These studies also revealed that the production of bone resorbing factors above described played important roles in the enlargement of jaw cyst and that these factors were derived from fibroblasts in cyst wall and were probably induced by mechanical stress。

A few researchers have suggested that the intracystic fluid pressure and/or several bone resorbing factors might concern with the jaw cyst enlargement. Therefore, in this study, the production of inflammatory cytokines and nitric oxide (NO) in cyst fluid and/or cyst wall were investigated because we speculated these pathophysiological substances might affect the functioh of osteoblasts and/or osteoclasts located in surrounding bone of jaw cyst. Results were as follows.1. Interleukin 6 (IL-6) and tumor necrosis factor alfa (TNF-α) were detected in the cyst wall tissue and it was confirmed that the fibroblast mainly produced both cytokines.2. Using RT-PCR analysis, the expressions of messenger RNA for inflammatory cytokines (IL-6, IL-8, TNF-α, IFN-γ, TGF-β1) and the recepter of cytokines and eicosanoids (IL-6R α, gp130, TNFR I, CXCR2) were detected in supernatants of cultured fibroblast from the radicular cyst wall.3. Interleukin beta (IL-β), transforming growth factor beta 1 (TGF- β 1) and macrophage colony stimulating factor (M-CSF) were detected in the cyst fluid. NO metabolites (NOィイD22ィエD2 /NOィイD23ィエD2) were detected in the cyst fluid obtained from radicular cysts and postoperative maxillary cysts.These results suggested that such pathophysiological substances as inflammatory cytokines, eicosanoids and nitric oxide metabolites were related to the mechanism of the resorption of surrounding bone adjacent to cyst wall. These studies also revealed that the production of bone resorbing factors above described played important roles in the enlargement of jaw cyst and that these factors were derived from fibroblasts in cyst wall and were probably induced by mechanical stress.