Research on factors regulating the activation of latently infection Epstein-Barr virus

潜伏感染EB病毒激活调控因素研究

• 批准号: 07457589
• 负责人: TAKADA Kenzo
• 金额: $ 0.96万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457589/
• 关键词: Epstein-Barr virus; latent infection; activation; anti-immunoglobulin antibody; promoter; 転写制御

This study aimed to identify factors regulating the expression of the BZLF1 gene which acts as the switch for shift from Epstein-Barr virus (EBV) latent infection to virus replicative cycle. Burkitt's lymphoma-derived Akata cells have a very unique property that cells are in a latent state of EBV infection and shift to virus replicative cycle upon treatment of cells by anti-immunoglobulin antibodies (anti-Ig). Recently, we have isolated EBV-negative subclones from parental Akata cells. By using EBV-negative Akata cells, we searched for cellular genes involved in virus induction from latent infection.Nuclear extract was isolated from anti-Ig-treated and -untreated cells and analyzed by a gel shift assay with probes specific for 3 BZLF1 promoter regions that had been shown to be important for BZLF1 expression (Shimizu et al., J.Virol.67 : 3240-3245,1993). We could see many bands, however the competition experiments revealed that none of them was specific. Further study with different conditions may allow to identify specifc bands.

本研究旨在确定调节 BZLF1 基因表达的因素，该基因充当从 Epstein-Barr 病毒 (EBV) 潜伏感染到病毒复制周期转变的开关。伯基特淋巴瘤来源的 Akata 细胞具有非常独特的特性，即细胞处于 EBV 感染的潜伏状态，并在用抗免疫球蛋白抗体（抗 Ig）处理细胞后转变为病毒复制周期。最近，我们从亲本 Akata 细胞中分离出 EBV 阴性亚克隆。通过使用 EBV 阴性 Akata 细胞，我们搜索了参与潜伏感染病毒诱导的细胞基因。从抗 Ig 处理和未处理的细胞中分离出核提取物，并通过凝胶转移测定法使用针对 3 个 BZLF1 启动子区域的特异性探针进行分析，这些启动子区域已被证明对 BZLF1 表达很重要（Shimizu 等人，J.Virol.67： 3240-3245，1993）。我们可以看到很多乐队，但竞争实验表明没有一个是特定的。在不同条件下的进一步研究可以识别特定的条带。

项目成果

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Koide, J.：“从类风湿性关节炎患者的滑膜组织中自发建立 Epstein Barr Vitus 感染的成纤维细胞系”J. Virol.71。

Wen, S.: "Association of Epstein-Barr virus(EBV)with sjogren's syndrome:differential EBV expression between epithelilal cells and lymphocytes in salivary glands." Am.J.Pathol. 149. 1511-1517 (1997)

Wen, S.：“EB 病毒 (EBV) 与干燥综合征的关联：唾液腺上皮细胞和淋巴细胞之间 EBV 表达的差异。”

Takada K.: "Pathogenic role of Epstein-Barr virus in human cancer." Intervirology. 38. 214-220 (1995)

Takada K.：“EB 病毒在人类癌症中的致病作用。”

Wen, S., Shimizu, N., Yoshiyama, H., Miyazaki, Y., Shinozaki, F.and Takada, K.: "Association of Epstein-Barr virus (EBV) with sjog ren's syndrome : differential EBV expression between epithelilal cells and lymphocytes in salivary glands." Am.J.Pathol.149.

Wen, S.、Shimizu, N.、Yoshiyama, H.、Miyazaki, Y.、Shinozaki, F. 和 Takada, K.：“EB 病毒 (EBV) 与干燥综合征的关联：上皮细胞之间的差异 EBV 表达

Yoshiyama, H. , Shimizu, N. and Takada, K.: "Persistent Epstein-Barr virus infection in a human T-cell line: unique program of latent virus expression." The EMBO Journal. 14. 3706-3711 (1995)

Yoshiyama, H.、Shimizu, N. 和 Takada, K.：“人类 T 细胞系中的持续 Epstein-Barr 病毒感染：潜在病毒表达的独特程序。”