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Development of a model animal for Epstein-Barr virus infection

EB病毒感染模型动物的研制

基本信息

批准号：
12557028
负责人：
TAKADA Kenzo
金额：
$ 8.45万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12557028/
关键词：
Epstein-Barr virus transgenic rat CD21 model animal receptor EBウイルス動物モデルクラスII

项目摘要

The human CD21 (hCD21) molecule, which is preferentially expressed on B-lymphocytes, is the receptor for EBV adsorption, and this determines the predominant B-lymphocyte tropism of EBV. B-lymphocytes from humans and some primates, but not from other species, are susceptible to EBV infection. We have recently demonstrated that introduction of the hCD21 gene makes some canine and rat cells susceptible to EBV infection and stably EBV-infected cell clones could be isolated from these cells. These results suggest that once the initial barrier to attachment is overcome, EBV can penetrate into and stably infect canine and rat cells. This finding has prompted us to generate a transgenic rat with the hCD21 gene, which could become an animal model for EBV infection. We generated transgenic rats expressing the human CD21 gene (hCD21) driven by the mouse immunoglobulin enhancer. Spleen B-lymphocytes expressing hCD21 were susceptible to Epstein-Barr virus (EBV) infection as evidenced by EBV latent gene expression, but the infection was abortive and was not followed by blastogenesis, cellular DNA synthesis or proliferation. The present findings indicate that human and rat lymphocytes respond to EBV infection differently in vitro. We are now studying whether the hCD21 transgenic rat could become a model animal for EBV infection.

人CD21 （hCD21）分子优先在b淋巴细胞上表达，是EBV吸附的受体，这决定了EBV主要的b淋巴细胞亲和性。人类和一些灵长类动物的b淋巴细胞易受EBV感染，而其他物种的b淋巴细胞则不然。我们最近已经证明，引入hCD21基因可以使一些犬和大鼠细胞对EBV感染敏感，并且可以从这些细胞中分离出稳定的EBV感染细胞克隆。这些结果表明，一旦克服了最初的附着障碍，EBV可以渗透并稳定地感染犬和大鼠的细胞。这一发现促使我们产生具有hCD21基因的转基因大鼠，这可能成为EBV感染的动物模型。我们在小鼠免疫球蛋白增强剂的驱动下产生了表达人类CD21基因（hCD21）的转基因大鼠。表达hCD21的脾脏b淋巴细胞易受eb病毒（EBV）感染，但感染失败，不发生胚发生、细胞DNA合成或增殖。目前的研究结果表明，人和大鼠淋巴细胞对EBV感染的体外反应不同。我们目前正在研究hCD21转基因大鼠能否成为EBV感染的模型动物。