Development and Application of Highly Sensitive Medical optodes

高灵敏医用光极的研制与应用

• 批准号: 07555572
• 负责人: SUZUKI Koji
• 金额: $ 2.43万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07555572/
• 关键词: chemical sensor; flow-through analysis; ionophore; ion-optode; optical fiber; waveguide; フロースルー分析; イオンセンサー; 光ファイバーセンサー; pHセンサー; オプティカルセンサー; クロモフォア

An optical chemical sensor is a device which can measure chemical species with optical transduction, and is called "Optode" or "Optrode". Optodes are now attacting much attention due to their high performance, especially their high sensitivity in practical applications, and the physicochemical and scientific interests of many researchers in the chemical, physical, and clinical fields.In the development of a highly sensitive optical fiber-type, ion-selective optodes based on newly designed and synthesized neutral ionophores using a newly developed low-loss polymer optical fiber, the response models were proposed and mathematical simulation of the response curves were investigated in order to elucidate the experimental response curves.For the application of these optodes, which were developed with immobilizing functional molecules on the sensor membranes, a novel methodology called "Rapid Flow-through Analysis (RFA)" was used. In this case the response theory was discussed in terms of th … More e optimization of the theoretical equation which is basically derived from Lambert-Beer's law and Fick's law of diffusion, based on the experimental responses obtained by utilizing novel pH optodes and sodium ion-selective optodes as the ion detection probes. Consequently, a novel analytical methodology was established which has the advantages of both rapid and high sensitive ion determination (RFA).On the other hand, an active waveguide sensor was designed as a novel highly sensitive ion detection probe. All planar waveguide sensors reported to date employed a waveguide as the only light propagating layr, however, the developed active waveguide optode, utilized the waveguide core, not only as the light propagation layr but also as the molecular recognition layr and transduction layr by inclusion of functional molecules such as neutral ionophores and a lipophilic dye. One of great merits of this waveguide sensor is that a high sensitivity can be obtained even using a miniaturize a probe. The potential applicability of this waveguide was confirmed by constructing two active waveguide ion optodes using a calcium selective ionophore and a sodium selective ionophore. A comparison of the experimental response curves obtained with novel waveguide optode and conventional waveguide optode based on evanescent wave spectrometry clarified that the sensitivity of the novel waveguide optode proposed here is apparently higher than that of the conventional waveguide optode. Less

光学化学传感器是一种可以通过光传导来测量化学物质的装置，被称为“Optode”或“Optrode”。光极由于其高性能，特别是在实际应用中的高灵敏度，以及化学、物理和临床领域许多研究人员的物理化学和科学兴趣而受到广泛关注。在使用新开发的低损耗聚合物光纤开发基于新设计和合成的中性离子载体的高灵敏度光纤型离子选择性光极时，提出了响应模型 为了阐明实验响应曲线，我们对响应曲线进行了研究和数学模拟。对于这些通过在传感器膜上固定功能分子而开发的光极的应用，使用了一种称为“快速流通分析（RFA）”的新颖方法。在这种情况下，根据利用新型 pH 光极和钠离子选择性光极作为离子检测探针获得的实验响应，根据理论方程的优化讨论了响应理论，该理论方程基本上源自兰伯特-比尔定律和菲克扩散定律。因此，建立了一种兼​​具快速和高灵敏度离子测定(RFA)优点的新型分析方法。另一方面，设计了有源波导传感器作为一种新型高灵敏度离子检测探针。迄今为止报道的所有平面波导传感器均采用波导作为唯一的光传播层，然而，开发的有源波导光极利用波导芯，不仅作为光传播层，而且通过包含功能分子（例如中性离子载体和亲脂性染料）作为分子识别层和转导层。该波导传感器的一大优点是即使使用小型化的探头也可以获得高灵敏度。通过使用钙选择性离子载体和钠选择性离子载体构造两个有源波导离子光极，证实了该波导的潜在适用性。通过对基于倏逝波谱法的新型波导光极和传统波导光极获得的实验响应曲线进行比较，可以看出本文提出的新型波导光极的灵敏度明显高于传统波导光极的灵敏度。较少的

项目成果

Hideaki Hisamoto: "Ion Sensitive and Selective Active Waveguide Optodes" Analytica Chimica Acta. in press (1997)

Hideaki Hisamoto：“离子敏感和选择性有源波导光极”分析化学学报。

Koji Suzuki: "Design and Synthesis of Highly Sodium Ion Selective Ionophores Based on 16-Crown-5 Derivatives for an Ion-Selective Electrode" Analytical Chemistry. 68. 208-215 (1996)

Koji Suzuki：“用于离子选择性电极的基于 16-Crown-5 衍生物的高钠离子选择性离子载体的设计和合成”分析化学。

Hideki Hisamoto: "Ion Sensitive and Selective Active Waveguide Optodes" Analytica Chimica Acta. (in press). (1997)

Hideki Hisamoto：“离子敏感和选择性有源波导光极”《分析化学学报》。

Dwi Siswanta: "Structural Ion Selectivity of Thia Crown Ether Compounds with a Bulky Block Subunit and their Application as an Ion Sensing Component for and Ion-Selective Electrode" Analytical Chemistry. 68. 4166-4172 (1996)

Dwi Siswanta：“具有大块亚基的硫冠醚化合物的结构离子选择性及其作为离子传感组件和离子选择性电极的应用”分析化学。

Hideaki Hisamoto: "Ion Sensitive and Selective Active Waveguide Optodes" Analytica Chimica Acta. (in press). (1997)

Hideaki Hisamoto：“离子敏感和选择性有源波导光极”分析化学学报。