Studies for High performance and Practical Use of Neutral Carrier-Type Ion Sensors Based on Silicone-Rubber Membranes

本工作的目的是研究基于硅橡胶膜和溶胶-凝胶衍生膜的中性载体型离子传感器的高性能和实用化。本文研究的离子传感器是离子敏感场效应晶体管(ISFET)和离子选择电极(ISE)。杯[4]芳烃离子载体主要用作钠的中性载体。研究结果如下：1)设计了具有结构不对称性和低聚(硅氧烷)基团的杯[4]芳烃离子载体，以获得在硅橡胶中高度分散的杯[4]芳烃离子载体。2)通过共价键将杯[4]芳烃离子载体等钠中性载体固定在硅橡胶膜上，以获得高耐久性，3)合成了含杯芳烃部分的低聚(二甲基硅氧烷)衍生物，以改善杯芳烃化学修饰硅橡胶膜的一些缺点；4)设计了有机-无机杂化有机-无机杂化膜；5)研究了烷氧基硅烷化中性载体溶胶-凝胶衍生膜对中性载体的改性。6)所研究的中性载体钠离子传感器具有良好的传感性能、耐久性和良好的性能和生物兼容性。并成功地应用于血清和尿液中钠的测定。

The aim of this work is to study for high performance and practical use of neutral-carrier-type ion sensors based on silicone-rubber membrane and sol-gel-derived membranes. The ion sensors studied here were ion-sensitive field-effect transistors (ISFET) and ion-selective electrodes (ISE). Calix [4] arene ionophores were mainly employed here as the sodium neutral carriers. The research results are as follows.1) Calix [4] arene ionophores incorporating structural unsymmetry and oligo (siloxane) group have been designed to obtain highly dispersible calixarene ionophore in silicone rubber.2) Sodium neutral carriers such as calix [4] arene ionophores have been successfully immobilized to silicone rubber membranes by covalent bonding, in order to attain highly durable, biocompatible neutral-carrier-based sensing membranes for ion-selectrodes.3) Oligo (dimethylsiloxane) derivatives carrying calixarene moiety have been synthesized to alleviate some drawbacks for the silicone-rubber membranes modified chemically with calixarene moiety.4) Sol-gel-derived membranes encapsulating a conventional neutral carrier such as valinomycin and bis (12-crown-4) have been designed as organic-inorganic hybrid ion-sensing membranes.5) Chemical modification of sol-gel-derived membranes with alkoxysilylated neutral carriers were attrmpted aiming at modification of glass membranes with neutral carriers.6) Any of the neutral carrier-type sodium ion sensors studied here showed high performance in sensor property, durability, and biocompatibility. Also, they have been sucessfully applied to sodium assay in human body fluid as sera and urine.