Efficient production and in vivo-effect of promoters of nerve growth factor-synthesis from edible mushrooms.

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1) Large scale preparation of known active compounds and search for novel active compounds from mushrooms.Some kinds of mushrooms (10-500kg) were extracted with ethanol. After removing the ethanol, the extracts were partitioned between organic solvents such as ethyl acetate or chloroform and water. The organic solvent-soluble parts were separated by repeated chromatography, giving known active compounds for in vivo assay. In addition, by screening of the activity against some mushroom extracts, it was revealed that some mushrooms promoted NGF-production. As a result, two new compounds, dictyophorine A and B were purified from a mushroom Dictyophora indusiata.2) Efficient production of active compounds.Production of erinacines A were examined under various culture conditions. As a results, an efficient condition was found (3g in 100 ml medium).3) In vivo experiments of hericenones and erinacines.The promoters of NGF-synthesis, hericenones and erinacines, have been isolated from a mushroom Hericium erinaceum. The in vivo effects of these compounds were examined using some model rats. The valuation of the effects is now in progress.