Efficient production and in vivo-effect of promoters of nerve growth factor-synthesis from edible mushrooms.

食用菌神经生长因子合成促进剂的高效生产及其体内作用。

• 批准号: 07556096
• 负责人: KAWAGISHI Hirokazu
• 金额: $ 3.71万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07556096/
• 关键词: Mushroom; Hericium erinaceum; Nerve growth factor; Mycelium; Fruiting body

1) Large scale preparation of known active compounds and search for novel active compounds from mushrooms.Some kinds of mushrooms (10-500kg) were extracted with ethanol. After removing the ethanol, the extracts were partitioned between organic solvents such as ethyl acetate or chloroform and water. The organic solvent-soluble parts were separated by repeated chromatography, giving known active compounds for in vivo assay. In addition, by screening of the activity against some mushroom extracts, it was revealed that some mushrooms promoted NGF-production. As a result, two new compounds, dictyophorine A and B were purified from a mushroom Dictyophora indusiata.2) Efficient production of active compounds.Production of erinacines A were examined under various culture conditions. As a results, an efficient condition was found (3g in 100 ml medium).3) In vivo experiments of hericenones and erinacines.The promoters of NGF-synthesis, hericenones and erinacines, have been isolated from a mushroom Hericium erinaceum. The in vivo effects of these compounds were examined using some model rats. The valuation of the effects is now in progress.

1)大规模制备已知的活性成分，寻找新的活性成分。除去乙醇后，将提取物在有机溶剂如乙酸乙酯或氯仿和水之间分配。通过重复色谱法分离有机溶剂可溶部分，得到用于体内测定的已知活性化合物。此外，通过对某些蘑菇提取物的活性筛选，发现某些蘑菇促进了NGF的产生。结果表明，从竹荪中分离得到了两个新化合物：竹荪碱A和B。2）有效成分的高效合成研究了不同培养条件下毛茛碱A的合成。3）猴头菌烯酮和猴头菌碱类化合物的体内实验从猴头菌（Hericium erinaceum）中分离得到了神经生长因子（NGF）合成的促进剂猴头菌烯酮和猴头菌碱类化合物。使用一些模型大鼠检查这些化合物的体内作用。对影响的评估正在进行中。

项目成果

H.Kawagishi: "Erinacines E F.and G,stimulators of nerve growth factor(NGF)-synthesis,from the mycelia of Hericium erinaceum." Tetrahedron Letters. 37・41. 7399-7402 (1996)

H. Kawagishi：“Erinacines E F. 和 G，来自猴头菌菌丝体的神经生长因子 (NGF) 合成刺激物。” 37・41 (1996)。

河岸洋和: "食品中の生体機能調節物質研究法" 学会出版センター, 191 (1996)

Hirokazu Kawagishi：“食品中生物功能调节剂的研究方法”学会出版中心，191（1996）

河岸洋和: "食品中の生体機能調節物質研究法" 学会出版センター, (199)

川岸博和：《食品中生物功能调节剂的研究方法》学会出版中心，（199）

河岸 洋和: "キノコの科学" 朝倉書店, 193 (1997)

川岸博和：《蘑菇的科学》朝仓书店，193（1997）

川岸洋和: "静岡のキノコ" 静岡新聞社, 237 (1997)

川岸博和：《静冈的蘑菇》静冈新闻社，237（1997）