Molecular breeding of crops that highly absorb nitrogen for the purpose ofsaving energy and for the environmental protection.

高吸氮作物的分子育种，以达到节能环保的目的。

• 批准号: 07556130
• 负责人: TAKEBA Go
• 金额: $ 2.62万
• 依托单位: Kyoto Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07556130/
• 关键词: nitrogen fertirizer; eutorophy; glutamine synthetase; rice plant; tobacco; nitrogen absorption; 形質転換

Transgenic tobacco plants with the cDNA encoding rice glutamine synthetase were createdusing an Agrobacterium-Ti plasmid vector. One of them showed very rapid growth in MSmedium. Transgenic rice plants with the same gene were also created by electroporationmethod. One of 26 transformants greatly absorbed nitrogen from paddy soil, and had 20stems that were three times more than a control plant. These data indicates that thebiotechnique can be used for the purpose of saving nitrogen fertilizer in the field andfor the environmental protection.

利用农杆菌Ti质粒载体构建了水稻谷氨酰胺合成酶基因的转基因烟草植株。其中一株在MS培养基中生长迅速。利用电转化法获得了转基因水稻植株。在26株转化植株中，有1株植株对土壤中的氮素有很强的吸收能力，其茎数为对照植株的3倍，达到20个。这些数据表明，生物技术可用于节省氮肥在田间和环境保护的目的。

项目成果

Teramoto,H.,E.Momotani,G.Takeba,H.Tsuji.: "Cloning of a cDNA for cyto-kinin-repressed gene in excised cucumber cotyledons." Planta. 193. 573-579 (1994)

Teramoto,H.,E.Momotani,G.Takeba,H.Tsuji.：“在切除的黄瓜子叶中克隆细胞分裂素抑制基因的 cDNA。”

木崎暁子、竹葉 剛: "植物の陸上化と光呼吸" 遺伝. 51・7. 9-11 (1997)

Akiko Kizaki，Tsuyoshi Takeha：“植物陆地化和光呼吸”遗传学 51・7（1997）。

Takeba,G.: "Stress Responses of Photosynthesis Organisms : Molecular Mechanisms and Molecular Regulations (eds.Satoh K.and Murata,N.)" Springer Verlag,Heidelberg, 21 (1998)

Takeba,G.：“光合作用生物体的应激反应：分子机制和分子调节（eds.Satoh K.和 Murata,N.）”Springer Verlag，海德堡，21（1998）

Takeba.G.: "Phoroprotective role of photorespiration." In The 34th NIBB conference″Responses of the photosynthetic apparatus to environmental light conditions.″. 130-132

Takeba.G.：“光呼吸的光保护作用。”第 34 届 NIBB 会议“光合装置对环境光条件的反应”130-132。

Teramoto,H., Toyama,T., Takeba,G. and Tsuji,H.: "Changes in expression of two cytokinin-repressed genes, CR9 and CR20, in relation to aging, greening and wounding in cucumber." Plauta. 196. 387-395 (1995)

寺本 H.、富山 T.、竹场 G.