Molecular mechanism of photorespiration by plastidic glutamine synthetase

质体谷氨酰胺合成酶光呼吸的分子机制

• 批准号: 08454259
• 负责人: TAKEBA Go
• 金额: $ 5.06万
• 依托单位: Kyoto Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08454259/
• 关键词: photosynthesis; photorespiration; glutamine synthetase; photodamage; photoinhibition; photooxidation; C3回路; CO_2; Rubisco; 活性酸素

Plants absorb light for photosynthesis but as light can itself be dangerous to plants, they need to protect themselves against its damaging effects. Here, we show that photorespiration can act as such a defence mechanism. We constructed transgenic tobacco plants enriched or reduced in plastidic glutamine synthetase (GS2), a key enzyme in photorespiration. Those transgenic plants having twice the normal amount of GS2 had an improved capacity for photorespiration and an increased tolerance to high-intensity light, whereas those with a reduced amount of GS2 gad a diminished capacity for photorespiration and were photoinhibited more severely by hight-intensity light compasred with control plants. We conclude that photorespiration protects C3 plants from photoinhibition.

植物吸收光进行光合作用，但由于光本身对植物是危险的，它们需要保护自己免受其破坏性影响。在这里，我们表明，光呼吸可以作为这样的防御机制。我们构建了富含或减少质体谷氨酰胺合成酶（GS 2）的转基因烟草植株，GS 2是光呼吸的关键酶。与对照相比，GS_2含量为正常水平两倍的转基因植株光呼吸能力增强，对强光的耐受性增强，而GS_2含量减少的转基因植株光呼吸能力减弱，强光光抑制更为严重。我们得出结论，光呼吸保护C3植物光抑制。

项目成果

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Takeba,G、 他: "Photorespiration is an essntial mechanism for the protection C3 plants from photooxidation in Stress Responses of Photosynthetic Organisms"Elsevir Amsterdam. 22 (1998)

Takeba, G. 等人：“光呼吸是保护 C3 植物免受光合生物体应激反应中光氧化的重要机制”Elsevir 阿姆斯特丹 22 (1998)。

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Kozaki, A., et al.: "Photorespiration protect C3 plants from photooxidation"Nature. 384. 557-560 (1996)

Kozaki, A. 等人：“光呼吸保护 C3 植物免受光氧化”《自然》。