Development of a rapid diagnosis system for the recurrent infectious constitutions.

开发针对复发性感染体质的快速诊断系统。

基本信息

批准号：
07557063
负责人：
MATUURA Nobuo
金额：
$ 8.19万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07557063/
关键词：
complement infirmative constitution recurrent infectious complement activation gene analysis mannan binding lectin (MBL)Ra-reactive factor (RaRF)gene diagnosis Mannan binding protein (MBP)Ra-reactive factor (RaRF)Maunose binding pr

项目摘要

1.Development of a rapid diagnosis system for estimation of serum P100 levels.Preparation of the P100 specific anti recombinant P100 antibodies : After reproduction of the recombinant proteins that fused with heavy or light chains of P100 and glutathion-S-transferase, the anti-recombinant P100 antibodies were prepared form immunized rabbit serum and assayd those specific activities. To determine of the serum P100 concentrations, we are performing the adjustment for the conditions of the sandwich method using anti-peptid and anti-recombinant antibodies.2.Development of a rapid diagnosis system for the P100 gene.Allotype analysis of the P100 gene : The nucleotide sequences of the P100 exons were direct determine from PCR amplified products that genome DNA was prepared from a normal adult peripheral blood lymphocyte as a template. Five allotypic amino acid substitutions derived from nucleotide mutations were identified comparisons with genomic and cDNA nucleotide sequences. Although screen for 300 genome DNA samples using mutation specific PCR primers that closely related to His exon, the mutations has not been find out. To the analysis for the survey of P100 genomic mutation, it is needed not only normal but recurrent infectious patients genome DNA.At the present, we are preparing those of genome DNA samples and searching for the presence of remained nucleotide mutatios. To the next step for the gene diagnosis system of P100, we are planning to screening conditions for the PCR-SSCP gene diagnostic method.

1.开发快速诊断系统，以估算血清P100水平。p100特定抗重组P100抗体的准备：重组蛋白与重组蛋白的重现后，与p100的重链或轻度链融合在一起，这些抗体和谷胱甘肽-S-抗转移酶的特定表现为这些抗体抗体，使其不合时宜地进行了不合时液。 To determine of the serum P100 concentrations, we are performing the adjustment for the conditions of the sandwich method using anti-peptid and anti-recombinant antibodies.2.Development of a rapid diagnosis system for the P100 gene.Allotype analysis of the P100 gene : The nucleotide sequences of the P100 exons were direct determine from PCR amplified products that genome DNA was prepared from a normal adult peripheral blood淋巴细胞作为模板。鉴定出与基因组和cDNA核苷酸序列进行比较的五个同种型氨基酸取代。尽管使用与外显子密切相关的突变特异性PCR引物进行了300个基因组DNA样品的筛选，但尚未发现突变。为了分析P100基因组突变的调查，不仅需要正常，而且需要复发性感染性患者基因组DNA。目前，我们正在准备基因组DNA样品的样本，并寻找剩余的核苷酸突变。在P100基因诊断系统的下一步中，我们计划筛选PCR-SSCP基因诊断方法的条件。