A study on visualization of nitriic oxide in humans

人体一氧化氮可视化研究

• 批准号: 07557185
• 负责人: IWAMOTO Jun
• 金额: $ 2.69万
• 依托单位: Asahikwa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07557185/
• 关键词: NITRIC OXIDE; AIRWAY; EPITHELIUM; COMPUTER; A-DCONVERSION; DATA ACQUISITION; オンライン測定; 信号同期; A-D変換; 化学発光; 潰瘍性大腸炎

In order to measure nitric oxide (NO) in human subjects, we have developed a realtime monitoring system especially design for a measurement of human exhaled NO.The system can exhibit not only the traces of exhaled NO (ppb) and CO2 but also air flow, VCO2, and VNO on the computer display. We can monitor the changes of exhlaed NO in a real time fashion. Visualization of NO is thus performed on the computer screen. This system consists of a chemiluminescence NO analyzer, an O2-CO2 analyzer, a gas flow meter, an AD/DA converting apparatus, signal delaying soft wear, a DA/AD converting apparatus, and the signal acquisition device (MacLab). The whole system was tested in the measurements of human exhaled NO and human nasal NO and turned out to be useful for the estimation of exhaled NO as well as nasal NO.We attempted to clarify the origin of exhaled NO in the human lower airway by using this device. The results demonstrated that the exhaled NO is likely to be produced in the peripheral airway, probably, terminal bronchioles. Because the exhaled NO increases when the expiration time and/or breath-hold time prolong and the alveolar ventilation increases. It was almost impossible to wash out NO in the airway by hyperventilation, suggesting that the NO is produced in the airway epithelia and continuously diffuses toward the airway cavity by its own high partial pressure within the epilthelia. Nasal NO was found to decrease by physical exercise along with a decrease in nasal airway resistance. In the clinical study, the exhaled NO from the patients with diffuse panbronchiolitis was measured to be very low. These patients exhibited low nasal NO as well. By using our device, accurate and quick measurement of exhaled NO was possible.

为了测量人体一氧化氮（NO），我们开发了专门为测量人体呼出一氧化氮而设计的实时监测系统。该系统不仅可以在计算机显示屏上显示呼出气一氧化氮（ppb）和二氧化碳的痕迹，还可以显示气流、VCO2和VNO。我们可以实时监测呼气NO的变化。因此，NO 的可视化是在计算机屏幕上进行的。该系统由化学发光NO分析仪、O2-CO2分析仪、气体流量计、AD/DA转换装置、信号延迟软磨损、DA/AD转换装置和信号采集装置(MacLab)组成。整个系统在人体呼出一氧化氮和人体鼻一氧化氮的测量中进行了测试，结果证明对于呼出一氧化氮和鼻一氧化氮的估计是有用的。我们试图通过使用该装置来澄清人体下呼吸道中呼出一氧化氮的来源。结果表明，呼出的一氧化氮很可能是在外周气道（可能是终末细支气管）中产生的。因为当呼气时间和/或屏气时间延长且肺泡通气量增加时，呼出的NO增加。通过过度换气几乎不可能洗掉气道中的NO，这表明NO是在气道上皮细胞中产生的，并通过其自身在上皮细胞内的高分压持续向气道腔扩散。研究发现，随着鼻气道阻力的降低，体育锻炼会降低鼻腔一氧化氮的含量。在临床研究中，测量弥漫性全细支气管炎患者呼出的一氧化氮非常低。这些患者的鼻腔 NO 也较低。通过使用我们的设备，可以准确、快速地测量呼出的一氧化氮。

项目成果

Yanagiya,N.: "Transient corneal edema induced by nitric oxide synthase inhibition." Nitric Oxide Biol.Chem.(Arch Biochem Biophys Part B). 1(5). 397-403 (1997)

Yanagiya,N.：“一氧化氮合酶抑制引起的短暂性角膜水肿。”

Yanagiya, N.: "Transient corneal edema induced by nitric oxide synthase inhibition." Nitirc Oxide Biol.Chem.(Arch Biochem Biophys Part B). 1(5). 397-403 (1997)

Yanagiya, N.：“一氧化氮合酶抑制引起的短暂性角膜水肿。”

Iwamoto, J.: "Analysis of single breath nitric oxide(SBVNO)in healthy subjects." FASEB J.11(3). A346- (1997)

Iwamoto, J.：“健康受试者单次呼吸一氧化氮 (SBVNO) 的分析。”

Ogawa,K.: "Nasal nitric oxide(NO)interferes measurement of exhaled NO during exercise." FASEB J.11(3). A51- (1997)

Okawa,K.：“鼻腔一氧化氮 (NO) 会干扰运动期间呼出一氧化氮的测量。”

Takahashi T,Nakano H,Iwamoto J,Ogawa K,Ide H,Akiba Y,Osanai S,Ogasa T,Kikuchi K.: "Reduction in nasal and exhaled NO in patients with diffuse panbronchiolitis (DPB)." Am J Resp crit Care Med. 155 (4). A823 (1997)

Takahashi T、Nakano H、Iwamoto J、Okawa K、Ide H、Akiba Y、Osanai S、Ogasa T、Kikuchi K.：“弥漫性全细支气管炎 (DPB) 患者鼻腔和呼出 NO 的减少。”