Physiological significance of exhaled nitric oxide during exercise

运动时呼出一氧化氮的生理意义

• 批准号: 07670077
• 负责人: IWAMOTO Jun
• 金额: $ 1.41万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670077/
• 关键词: NITRIC OXIDE; NASAL RESISTANCE; EXERCISE; EXHALED GAS; 化学発光; 呼気NO

While the exhaled gas passes through upper airway and nasal cavity, i t acquires nitric oxide (NO) in these sites. The final origin of NO in the airway is not clear, but it seems that NO may diffuse out of the airway epithelia to the lumens. In order to estimate the outflux of NO in the airway, we have measured the exhaled NO from the nasal cavity and mouth separately. For this purpose we have developed a real-time measurement system for exhaled NO.This device can automatically measure both NO concentration and VNO.VNO,of which unit is nl/min, is a production rate for exhaled NO.This parameter is the only useful tool to estimate the amount of NO from the airway.1. Nasal NOWe studied human nasal NO in healthy volunteers. The subject was asked to perform a voluntary closure of soft palate (VCSP) in order to ventilate nasal airway from the right (or left) nostril to the left (or right). The VNO of the nose was measured approximately 300 nl/min. After 4 min of moderate exercise, the VNO wa … More s significantly decreased by 30%. The close measurement of NO in the nose with an inserted tube revealed that the nasal NO has pattern of oscillation which synchronized with that of pulsatile changes of nasal mucosal blood flow. These results suggest that the nasal NO is arising from epithelia and/or vascular bed of nasal mucosa.2. NO from lower airwayThe real-time measurement clarified many unknown aspects of exhaled NO.The pattern of single breath of exhaled NO (VNO) is always peaked at the early period of expiration (0.1 to 0.3 sec after beginning of expiration) and tapered abruptly in the end-expiratory period. This suggests that NO in the lower airway may originate in the sites close to the alveolus, probably periphery to terminal bronchioles. The breath hold can increase VNO simply depending on the breath hold time. The linear relationship between VNO and the time strongly suggests that NO is continuously sprung from the airway wall at constant rate. Another interesting finding was that alveolar ventilation is correlated with VNO.This suggests that NO is picked up by the alveolar gas while it is passing by. In conclusion, NO production in the airway is stable and continuous during resting and measurable in a unit of VNO. Less

呼出的气体通过上呼吸道和鼻腔时，在这些部位获得一氧化氮(NO)。NO在呼吸道中的最终来源尚不清楚，但似乎NO可能从呼吸道上皮扩散到管腔。为了估计NO在呼吸道中的流出量，我们分别测量了鼻腔和口腔呼出的NO。为此，我们开发了一套呼出NO的实时测量系统。该装置既可以自动测量NO浓度，也可以自动测量VNO。VNO是呼出NO的产生率，单位为NL/min。这个参数是估计呼吸道NO量的唯一有用的工具1。鼻部NOWE研究了健康志愿者的鼻部NO。受试者被要求进行软腭部自愿性闭合(VCSP)，以便从右(或左)鼻孔向左(或右)鼻孔通气。测得鼻的VNO约为300nL/min。在适度运动4分钟后，Vno Wa…更多的S则大幅减少了30%。用插管法对鼻腔内NO进行近距离测量，发现鼻腔内NO具有与鼻黏膜血流量脉动性变化同步的振荡模式。这些结果提示，鼻腔NO来源于鼻黏膜上皮细胞和/或血管床。呼出NO的单次呼出模式(VNO)总是在呼气早期(呼气开始后0.1-0.3秒)达到峰值，在呼气末突然下降。提示下呼吸道NO可能起源于接近肺泡的部位，可能位于末梢细支气管处。屏气可以简单地根据屏气时间来增加VNO。VNO与时间之间的线性关系强烈地表明，NO以恒定的速率从气道壁持续涌出。另一个有趣的发现是，肺泡通气量与肺活量相关。这表明，当肺泡气经过时，肺泡气会吸收NO。综上所述，在静息状态下，气道中NO的产生是稳定和持续的，并且可以在单位VNO中测量到。较少

项目成果

Iwamoto, J., Imada, M., Unno, T., Nonaka, S., and A.Kuroshima: "Nasal nitric oxide decreases along with nasal airway resistance after exercise." Jpn.J.Physiol. 45 (suppl. 2). S280 (1995)

Iwamoto, J.、Imada, M.、Unno, T.、Nonaka, S. 和 A.Kuroshima：“运动后，鼻腔一氧化氮随着鼻气道阻力下降。”

Iwamoto, J.: "The physiological basis for NO therapy: factors that may affect effectiveness of exogenous NO" Program & Abstracts Fourth Congress of Asia Pacific Association for Raspiratory Care Seventh Seminar for Respiratory Care. 201-205 (1995)

Iwamoto, J.：“NO 疗法的生理学基础：可能影响外源性 NO 有效性的因素”计划

Ogasa,T.: "Blood attenuates flow-dependent increase in exhaled NO in buffer-perfused rabbit lungs." Am J Resp Crit Care Med. 153(4). A186 (1996)

Ogasa,T.：“血液减弱了缓冲液灌注的兔肺中呼出一氧化氮的流量依赖性增加。”

Iwamoto,J.: "Pulsatile excretion of human nasal nitric oxide measured by a fast real-time detection system." Am J Resp Crit Care Med. 153(4). A851 (1996)

Iwamoto,J.：“通过快速实时检测系统测量人鼻一氧化氮的脉动排泄。”

Imada M: "Measurement of nitric oxide in human nasal airway." Eur.Respir.J.9. 556-559 (1996)

Imada M：“人鼻气道中一氧化氮的测量。”