The application of n-3 fatty acid-containing phospholipids as functional food.

含n-3脂肪酸磷脂作为功能食品的应用

• 批准号: 07558002
• 负责人: MASUZAWA Yasuo
• 金额: $ 8.58万
• 依托单位: HYOGO UNIVERSITY OF TEACHER EDUCATION
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07558002/
• 关键词: n-3 fatty acid; docosahexaenoic acid; eicosapentaenoic acid; phospholipid; nutrition; stability; hydroperoxide; autoxidation; ドコサヘキサエン酸; 組織脂肪酸組成; コレステロール; EPA産生細菌

1. Highly DHA-containing phospholipid (DHA-PL) was administered to rats and the ability to alter the fatty acid composition of the tissue lipids was compared with that of triglyceride (TG). The results indicate that n-3 fatty acid containing PL more efficiently decreases n-6/n-3 fatty acid ratio of serum and blood cells than TG.2. The stability of didocosahexaenoyl-GPC (DHA-PC) against peroxidation was assessed in comparison with that of tridocosahexaenoate (DHA-TG). When DHA-PC was autoxidised in glass tubes without solvent, apparent generation of PL-hydoperoxide was approximately 1/100 of that of TG-hydoperoxide. However the half-life period of DHA-PL was less 1/10 of that of DHA-TG,indicating that DHA-PC was much less stable in oxidative degradation than DHA-TG,but that PC-hydroperoxide level was much less than that of TG-hydroperoxide.3. Conclusion ; The stability of lipids against hydroperoxidation has been considered to be depend on the number of double bond of fatty acid residue. However our results indicate that rate of oxidative degradation of lipids also depends on the chemical form of fatty acid ester and that phospholipids as food shpuld be more carefully handled or stored to prevent oxidative degradation than TG.Also nutritional effects of PC form of n-3 fatty acids may be different from that of TG form and n-3 fatty acid-containing PL may be more useful to prevent thrombosis and related heart diseases than other chemical form of n-3 fatty acids.

1.高DHA含磷脂（DHA-PL）给药大鼠和改变组织脂质的脂肪酸组成的能力进行了比较，与甘油三酯（TG）。结果表明，含n-3脂肪酸的PL比TG更有效地降低血清和血细胞的n-6/n-3脂肪酸比值。比较了二十二碳六烯酸酯（DHA-TG）和二十二碳六烯酰-GPC（DHA-PC）对过氧化反应的稳定性。当DHA-PC在无溶剂的玻璃管中自氧化时，PL-过氧化氢的表观生成量约为TG-过氧化氢的1/100。但DHA-PL的半衰期小于DHA-TG的1/10，说明DHA-PC的氧化降解稳定性远低于DHA-TG，但PC的过氧化氢水平远低于TG。结论：脂质抗过氧化作用的稳定性主要取决于脂肪酸残基中双键的数目。然而，我们的研究结果表明，脂质的氧化降解速率也取决于脂肪酸酯的化学形式，并且磷脂作为食物应比TG更小心地处理或储存以防止氧化降解。此外，PC形式的n-3脂肪酸的营养作用可能不同于TG形式和n-3脂肪酸。与其它化学形式的n-3脂肪酸相比，含有PL的化合物可能更有助于预防血栓形成和相关的心脏病。