ケージド化合物励起用UV照射・調光装置の開発的研究

笼状化合物激发紫外辐照及调光装置的进展研究

• 批准号: 07558112
• 负责人: KURAHASHI Takashi
• 金额: $ 6.91万
• 依托单位: Osaka University (1996-1997)Okazaki National Research Institutes (1995)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07558112/
• 关键词: Caged compound; cAMP; ionic channel; Patch clamp; UV flash; Olfactory cell; Retina; computer program; 味細胞; コンピュータプログラム

Olfactory transduction is mediated by the adenylyl cyclase-cAMP system, and their adaptation is regulated by Ca-feedback within the cAMP metabolism. In a qualitative manner, outline of olfactory transduction system is now fairly well understood. However, in the view of quantification, there are still many unresolved questions, since in the field of olfaction research, it is so difficult to control the stimulus delivery In the present study, for response quantification, we aimed to quantify the stimulation to the olfactory receptor cell. To do so, we used caged cAMP and developed a new UV-flash source which can control UV intensity freely and rapidly. Using the whole-cell patch clamp method, we monitored membrane currents sensitive to cytoplasmic cAMP.Photolysis of cytoplasmic caged-cAMP caused rapid activation of cAMP-gated channels. Using this new system, we are planning to quantify the response shape and Ca-dependence of the adaptation in the olfactory receptor cell. In future, the same protocol will be used for the understanding of wide variety of cell functions.

嗅觉转导是由腺苷酸环化酶-cAMP系统介导的，它们的适应是由cAMP代谢中的ca反馈调节的。从定性的角度来说，嗅觉转导系统的轮廓现在已经相当清楚了。然而，从定量的角度来看，由于在嗅觉研究领域中，刺激传递的控制非常困难，因此仍有许多未解决的问题。在本研究中，我们的目标是对嗅觉受体细胞的刺激进行定量。为此，我们采用笼型cAMP，开发了一种可以自由、快速控制紫外线强度的新型紫外闪光源。利用全细胞膜片钳法，我们监测了对细胞质cAMP敏感的膜电流。胞质笼型camp光解引起camp门控通道的快速激活。利用这个新系统，我们计划量化嗅觉受体细胞适应的响应形状和钙依赖性。在未来，同样的方案将用于了解各种各样的细胞功能。

项目成果

倉橋 隆(共著): "「脳と神経」章 視覚と嗅覚の情報変換機構" 共立出版,

仓桥隆（合著者）：《‘大脑和神经’篇视觉和嗅觉的信息转换机制》Kyoritsu Shuppan，

Kurahashi T & Menini A: "Mechanism of Odorant adaptation in the afactry receptor cell" Nature. 385. 725-729 (1997)

仓桥T

Kurahashi T & Menini A: "Mechanism of Odorant Adaptation in the Olfactory Receptor Cell" Nature. 385. 725-729 (1997)

仓桥T

倉橋 隆(共著): "シリーズ・ニューバイオフィジックス6.生物のスーパーセンサー「匂いのセンサー」" 共立出版, 44-53 (1997)

Takashi Kurahashi（合著者）：“系列新生物物理学 6. 生物超级传感器‘气味传感器’”Kyoritsu Shuppan，44-53 (1997)