Mechanisms of transduction system in the olfactory cilia

嗅觉纤毛转导系统的机制

• 批准号: 22500357
• 负责人: KURAHASHI Takashi
• 金额: $ 2.66万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22500357/
• 关键词: 嗅覚マスキング; LogD; 匂い分子; CNGチャネル; 光活性化物質; 神経生理学; 電気生理学; パッチクランプ法; 嗅細胞; 脂溶性; 細胞培養; UVレーザー

Molecular diffusion in solutions is known to occur in the general neurons and cells. However, olfactory receptor cells have cilia, which having a nano-scale structure. In fact, olfactory transduction cascade exists within the cilia locally, it is important to know the spatial distribution and temporal kinetics of the transduction elements (cAMP and Ca2+) in the transduction cascade within the cilia in real-time. However, there were technical difficulties using nano-scale cilia. In the previous report, we overcame such technical limitation using patch clamp, nano-scale ROI, and fluorescent imaging (Fluo3 or 4) simultaneously. These results indicated that olfactory response was linear-summation at local in the cilia, which was caused by the existence of the diffusion limitation in the cilia. The olfactory masking showed presumably representing the integration of the substance into the hydrophobic site of the membrane.

已知溶液中的分子扩散发生在一般的神经元和细胞中。然而，嗅觉受体细胞具有纤毛，具有纳米级结构。实际上，嗅觉转导级联在当地的纤毛中存在，重要的是要了解cilia内部cascade cascade cascade cascade元素的空间分布和时间动力学。但是，使用纳米级纤毛遇到了技术困难。在上一个报告中，我们同时使用贴片夹，纳米级ROI和荧光成像（Fluo3或4）克服了这种技术限制。这些结果表明，嗅觉响应是纤毛中局部的线性召唤，这是由于纤毛中存在扩散限制引起的。嗅觉遮罩可能表明代表该物质在膜的疏水位点的整合。

项目成果

Recordings from cultured newt olfactory receptor cells.

来自培养的蝾螈嗅觉受体细胞的记录。

• 发表时间: 2012
• 期刊: Zoolog Sci.
• 作者: Matsumura K;Matsumoto M;Kurahashi T;Takeuchi H.
• 通讯作者: Takeuchi H.

嗅覚初期過程の分子機構

早期嗅觉过程的分子机制

• 发表时间: 2012
• 作者: Kurahashi;T.;竹内裕子 倉橋隆;竹内裕子・倉橋隆;倉橋 隆;倉橋 隆
• 通讯作者: 倉橋 隆

嗅細胞の電位依存性Kチャネルにおける匂い物質による抑制

气味剂对嗅觉细胞电压门控 K 通道的抑制

• 发表时间: 2010
• 作者: Hiroko Takeuchi;Takashi Kurahashi;倉橋隆;倉橋隆;倉橋隆;倉橋隆;倉橋隆;吉田太一・竹内裕子・倉橋隆
• 通讯作者: 吉田太一・竹内裕子・倉橋隆

Chemical structures of odorants that suppress ion channels in the olfactory receptor cell

• DOI: 10.1007/s12576-011-0142-2
• 发表时间: 2011-03
• 期刊: The Journal of Physiological Sciences
• 作者: Yukako Kishino;Hiroyuki Kato;T. Kurahashi;H. Takeuchi

Suppression and recovery of voltage-gated currents after cocaine treatments of olfactory receptor cells

可卡因处理嗅觉受体细胞后电压门控电流的抑制和恢复

• DOI: 10.1016/j.anl.2011.11.002
• 发表时间: 2012
• 期刊: Auris Nasus Larynx
• 影响因子: 1.7
• 作者: Tamari K;Takeuchi H;Kobayashi M;Kurahashi T;Yamamoto T
• 通讯作者: Yamamoto T