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Analysis of retinal information processing with multi-electrode recordings

多电极记录视网膜信息处理分析

基本信息

批准号：
07558291
负责人：
TACHIBANA Masao
金额：
$ 2.56万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07558291/
关键词：
retina retinal ganglion cell light adaptation receptive field multi-electrode vision 神経節細胞並列情報処理

项目摘要

To analyze the visual information processing in the vertebrate retina, light-induced spike discharges of retinal ganglion cells were recorded extracellularly using a multi-electrode array. The planar electrode array was fabricated by the following methods. A sputtered film of the transparent conductor indium thin oxide (ITO) was patterned on a silica glass wafer by photolithography, using wet etching in HCl. A insulating layr of Al_2O_3 was then applied by evaporation to the whole substrate, but lifted off at the tips of the ITO electrodes by photolithographic patterning. The exposed electrode tips were platinized by electrolytic deposition. A piece of retina was isolated in darkness from a frog, and was mounted on the multi-electrode array. Light stimuli were projected from a computer monitor on the retinal photoreceptor layr. Patterns consisted of a random pixel pattern with independently modulated square fields or a single uniform field, the intensity of which was modulated sinusoidally in the temporal domain. Data were aquired from ganglion cells, which responded phasically to dimming stimuli (dimming cells). Spatio-temporal receptive fields were obtained by analyzing the correlations between each spike discharge and the preceded random pixel pattern. Light adaptation changed their temporal property but not their spatial property. Auto-and cross-correlation analyzes were carried out in spike discharges evoked by a sinusoidally modulated uniform field. The results suggested that dimming cells receive a common input from spiking neurons with large receptive field, which seemed to be amacrine cells.

为了分析脊椎动物视网膜的视觉信息处理，利用多电极阵列记录了视网膜神经节细胞的光诱导峰放电。采用以下方法制备了平面电极阵列。采用HCl湿蚀刻技术，将透明导体氧化铟薄（ITO）溅射薄膜刻印在硅玻璃晶圆上。然后通过蒸发将Al_2O_3绝缘层涂在整个衬底上，但通过光刻图案化在ITO电极的尖端处升起。裸露的电极尖端采用电解沉积镀铂。在黑暗中从青蛙身上分离出一块视网膜，并安装在多电极阵列上。光刺激从计算机显示器投射到视网膜感光层上。图案由随机像素图案组成，具有独立调制的方形场或单个均匀场，其强度在时域中进行正弦调制。数据是从神经节细胞获得的，神经节细胞对调光刺激有阶段性反应（调光细胞）。通过分析每个脉冲放电与之前的随机像素模式的相关性，获得了时空感受野。光适应改变了它们的时间特性，但没有改变它们的空间特性。对正弦调制均匀场诱发的尖峰放电进行了自相关分析和互相关分析。结果表明，变暗细胞从具有大接受野的尖峰神经元接收共同输入，这些神经元似乎是无突细胞。