Functional anatomy of nonspiking interneuron dendrites

非尖峰中间神经元树突的功能解剖学

• 批准号: 07640894
• 负责人: TAKAHATA Masakazu
• 金额: $ 1.54万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07640894/
• 关键词: Crayfish; Sensory nonspiking interneuron; Premotor nonspiking interneuron; Dendrites; Confocal laser scanning microscope; Synaptic integration; Electrotonic structure; Passive membrane properties; シナプス統合機能; 解剖学的構造; ノンスパイキング介在神経; 形態計測; 計算機シミュレー

The structure of sensory and premotor nonspiking interneurons in the terminal abdominal ganglion of the crayfich, Procambarus clarkii Girard, has been studied quantitatively by 3-dimensional morphometry, using a confocal laser scanning microscopic system. Their passive membrane properties have also been studied electrophysiologically to analyze their electrotonic structure. Two types of premotor nonspiking interneurons, AL- and PL-types that are characterized by different structure of major dendrites and different location of cell bodies in the ganglion, have been found to share common dendritic field in the neuropil. They are different from each other both morphologically and physiologically : the PL-type cells show a larger number of branching, anatomically longer fine (<3 mm) dendrites and shorter time and length constants (p<0.05). The total electrotonic length of fine dendrites is accordingly significantly longer in PL- than in AL-type cells. The total anatomical and electrotonic lengths of whole dendrites were not statistically different between them (P>0.05). An identified sensory interneuron had significantly shorter membrane time constant and length constant. Comparison of morphological and electrophysiological properties between premotor and sensory nonspiking interneurons has revealed that the total anatomical length of thick dendrites (>3 mm) is greater in the sensory than in premotor cells whereas the time and length constants are smaller in the former (P<0.05). The total electrotonic lengths of both thick and fine dendrites of the sensory interneuron were significantly greater than those of premotor cells (P<0.05). The difference in electrotonic structure between sensory and premotor nonspiking interneurons seems to reflect their different manners of synaptic integration in the control of postsynaptic nerve cells.

应用激光共聚焦扫描显微镜系统，对克氏原螯虾（Procambarusclarkii吉拉德）终末腹神经节感觉和运动前非棘波中间神经元的结构进行了定量研究。它们的被动膜特性也被电生理学研究，以分析它们的电紧张性结构。两种类型的前运动nonspiking interneurons，AL-和PL-类型，其特征在于不同的结构的主要树突和不同的位置的细胞体在神经节，已被发现共享共同的树突领域的神经元。它们在形态学和生理学上彼此不同：PL型细胞显示出更多的分支，解剖学上更长的细（<3 mm）树突和更短的时间和长度常数（p<0.05）。因此，总电紧张长度的细树突是显着长于PL-比AL型细胞。两组树突的总长度和电紧张长度差异无统计学意义（P>0.05）。一个确定的感觉中间神经元有显着较短的膜时间常数和长度常数。比较运动前和感觉非棘突中间神经元的形态学和电生理特性，发现感觉神经元粗树突（>3 mm）的总长度大于运动前神经元，而时间常数和长度常数则小于运动前神经元（P<0.05）。感觉中间神经元粗树突和细树突的总电紧张长度均显著大于运动前细胞（P<0.05）。感觉和运动前非尖峰中间神经元之间电紧张结构的差异似乎反映了它们在控制突触后神经细胞中突触整合的不同方式。

项目成果

Maki Murayma: "Sensory control mechanisms of the uropod equilibrium reflex during walking in the crayfish Procambarus clarkii" Journal of Experimental Biology. 199. 521-528 (1996)

Maki Murayma：“克氏原螯虾行走时尾足平衡反射的感觉控制机制”实验生物学杂志。