Physiological analyzes of synaptic mechanisms underlying the central compensation in crayfish using simultaneous intracellular recording techniques

使用同时细胞内记录技术对小龙虾中枢补偿的突触机制进行生理分析

• 批准号: 14340260
• 负责人: TAKAHATA Masakazu
• 金额: $ 5.82万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14340260/
• 关键词: Crustaceans; Statocyst; Postural control; Central compensation; Nonspiking giant interneurons; Local interneurons; Crayfish; Simultaneous Intracellular Recording; 局在介在ニューロン; 細胞内記録・染色法; NGI; 前大脳; 中大脳; シナプス接続; 甲殻類; 樹状突起; シナプス

When the equilibrium organ of crustaceans is partially disabled, the eyestalks first take bilaterally asymmetrical posture, but they gradually recover the original symmetrical posture in a few weeks. Although this recovery process has long been known as the central compensation at the behavioral level, the physiological process subserving it largely remains unknown at the neuronal level. In this study, the synaptic mechanisms underlying the central compensation of the eyestalk posture following unilateral statolith removal have been investigated using intracellular recording and staining techniques in crayfish. Physiological analyzes of the dendritic membrane properties and synaptic activities of nonspiking giant interneurons (NGIs) that play a crucial role in the sensori-motor signal transmission for controlling the eyestalk posture revealed that not only their passive membrane properties but also the spontaneous synaptic activities showed significant changes during the compensation p … More rocess. The finding that the input resistance and membrane time constant increased or decreased after unilateral statolith removal, depending on the side of operation, clearly demonstrated that NGIs are the site of changes leading to the central compensation. However, since the spontaneous synaptic activity of NGIs reflects the spike activity of those cells that are presynaptic to NGIs, it was suggested that those presynaptic interneurons also play important roles in the central compensation. Many local interneurons that made either monosynaptic or polysynaptic connection with NGIs were identified by simultaneous intracellular recording from them. Most of these local interneurons projected dendritic branches onto the parolfactory lobe of the deutocerebrum where statocyst afferents also project directly as well as onto the protocerebrum where dendrites of NGIs are located, thus mediating statocyst information to NGIs. It is concluded that the central compensation process is based on activity changes at multiple sites in the brain including NGIs and their presynaptic cells identified in this study. Less

当甲壳类动物的平衡器官部分失能时，眼柄首先采取两侧不对称的姿势，但在几周内逐渐恢复原来的对称姿势。虽然这种恢复过程长期以来一直被认为是行为水平上的中枢补偿，但在神经元水平上，辅助它的生理过程在很大程度上仍然是未知的。在这项研究中，突触机制的基础上的中央补偿眼柄的立场后，单侧耳石删除已调查使用细胞内记录和染色技术在螯虾。对在控制眼柄姿势的感觉-运动信号传递中起关键作用的非尖峰巨中间神经元（NGIs）的树突膜特性和突触活动的生理分析表明，在代偿期，不仅其被动膜特性，而且其自发突触活动也发生了显著变化。 ...更多信息 过程。单侧耳石摘除后，输入电阻和膜时间常数增加或减少，这一发现取决于手术侧，清楚地表明NGIs是导致中枢代偿的变化部位。然而，由于NGIs的自发突触活动反映了那些与NGIs突触前的细胞的尖峰活动，因此建议这些突触前中间神经元在中枢补偿中也起重要作用。通过细胞内同步记录，发现许多与NGIs形成单突触或多突触连接的局部中间神经元。大多数这些本地interneurons投射树突分支到中脑的旁嗅叶，平衡囊传入也直接投射到前脑，NGI树突所在的地方，从而调解平衡囊信息NGI。得出的结论是，中央补偿过程是基于活动的变化，在多个网站在大脑中，包括NGIs和他们的突触前细胞在这项研究中确定。少

项目成果

Hiraguchi, T., Yamaguchi, T., Takahata, M.: "Mechanoreceptors involved in the hindwing-evoked escape behaviour in cricket, Gryllus bimaculatus"Journal of Experimental Biology. 206. 523-534 (2003)

Hiraguchi, T.、Yamaguchi, T.、Takahata, M.：“机械感受器参与蟋蟀后翅诱发的逃逸行为，Gryllus bimaculatus”实验生物学杂志。

Effects of leg movements on the synaptic activity of descending statocyst interneurons in crayfish, Procambarus clarkii.

腿部运动对克氏原螯虾降行静态囊中间神经元突触活动的影响。

• 发表时间: 2003
• 期刊: J.Comp.Physiol. 189
• 作者: Sato;N.;Kawakami;T.;Nakayama;A.;Suzuki;M.;Kasahara;H.;Obinata;T;荒木 崇(分担執筆);綿野 泰行;N.Hama
• 通讯作者: N.Hama

Mechanoreceptors involved in the hindwing-evoked escape behaviour in cricket Gryllus bimaculatus.

机械感受器参与蟋蟀双斑蟋蟀后翅诱发的逃逸行为。

• 发表时间: 2003
• 期刊: J.Exp.Biol. 206
• 作者: Hiraguchi;T.
• 通讯作者: T.

Mechanoreceptors involved in the hindwing-evoked escape behavior in cricket, Gryllus bimaculatus

机械感受器参与蟋蟀后翅诱发的逃逸行为

• 发表时间: 2003
• 期刊: J.Exp.Biol. 206
• 作者: T.Hiraguchi

Modification of statocyst input to local interneurons by behavioral condition in the crayfish brain

• DOI: 10.1007/s00359-005-0630-z
• 发表时间: 2005-08-01
• 期刊: JOURNAL OF COMPARATIVE PHYSIOLOGY A-NEUROETHOLOGY SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY
• 影响因子: 2.1
• 作者: Hama, N;Takahata, M
• 通讯作者: Takahata, M