Development of New Fluorescence Derivatization Reagents with 6-Aminoquinoxalines

新型6-氨基喹喔啉荧光衍生试剂的研制

基本信息

批准号：
07651038
负责人：
KATOH Akira
金额：
$ 1.34万
依托单位：
Seikei University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

There are trace amounts of bioactive substances such as carboxylic acids, alcohols, and amines in the body. The determination of them is quite significant for elucidation of physiological function, diagnosis of sickness, explication of cause of disease, and medical treatment. Therefore, many fluorescence derivatization reagents have been developed for the determination of these substances by high-performance liquid chromatography (HPLS). In this project, I investigated new fluorescent 6-aminoquinoxalines and their application to fluorescence derivatization reagents for carboxylic acids.1.2,3-Disubstituted 6-nitroquinoxalines were obtained by treatment of 2,3-dichloro-6-nitroquinoxaline with some nucleophiles such as amine, alcochol, and crown ether in high yields. 2. The catalytic hydrogenation with 10% Pd-C afforded fluorescent 6-aminoquinoxalines. 3.6-Aminoquinoxalines were coupled with long-chain carboxylic acids by means of the dicyclohexylcarbodiimide (DCC)-1-hydroxybenzotriazol (HOBt) method t give the corresponding 6- (acyl) aminoquinoxalines. 4.6- (Acyl) aminoquinoxalines were monitored by reversed phase HPLC equipped with a fluorescence detector (lambdaex 330 nm, lambdaem 395 nm) by using a column packed with a Finepak SIL C_<12>S.5. All the peaks were completely separated within 40 min. 6. The detection limit of these N-acyl derivatives were found to be 1.0 fmol/mul (injection volume).In conclusion, 2,3-disubstituted 6-aminoquinoxalines have been demonstrated to be applicable to new fluorescence derivatization reagents for the carboxylic acids.

体内有许多痕量的生物活性物质，例如羧酸，醇和胺。确定它们对于阐明生理功能，疾病诊断，疾病原因和医疗治疗非常重要。因此，已经开发了许多荧光衍生物试剂，用于通过高性能液相色谱（HPL）来测定这些物质。 In this project, I investigated new fluorescent 6-aminoquinoxalines and their application to fluorescence derivatization reagents for carboxylic acids.1.2,3-Disubstituted 6-nitroquinoxalines were obtained by treatment of 2,3-dichloro-6-nitroquinoxaline with some nucleophiles such as amine, alcochol, and crown ether in high yields. 2。使用10％PD-C的催化氢化提供荧光6-氨基喹啉。 3.6-氨基喹啉与长链羧酸通过双环己基钙二二酰亚胺（DCC）-1-羟基苯并二唑（HOBT）方法T提供相应的6-（ACYL）氨基喹啉碱。通过使用装有FinePak Sil C_ <12> S.5的柱子，通过配备有荧光检测器（Lambdaex 330 nm，lambdaem 395 nm）的反向相的HPLC监测4.6-（酰基）氨基喹啉。所有峰在40分钟内完全分离。 6。发现这些N-酰基衍生物的检测极限为1.0 FMOL/MUL（注射体积）。结论，已证明2,3-二取代的6-氨基喹氧化物适用于羧酸的新荧光衍生物试剂。