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ADVENTITIOUS BUD FORMATION FROM INTERCALARY MERISTEM AND MASS PROPAGATION OF HERBACEOUS PLANTS.

间生芽的形成和草本植物的大量繁殖。

基本信息

批准号：
07660036
负责人：
HOSOKI Takashi
金额：
$ 1.41万
依托单位：
SHIMANE UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660036/
关键词：
MS medium Hyponex BA IBA Node culture shoot split method herbaceous plants propagation オーキシンサイトカイニンオウゴンヤグルマ草ドイツアザミキキョウオミナエシ MS 節切片スイトピー

项目摘要

Mass propagationof of herbaceous plants was coducted using nodal tissue because the tissue has capacity to produce axillary and adventitious buds. The methology btained here was very simple and convinient. Namely, shoot apices was cultured on MS medium containing 0,0.1 and 1 ppm BA for 2-3 weeks. Then stem with nodes was traversely cut or vertically if stem did not elongate. The cut pieces were subcultured on the same media and after 2-3 weeks, they were cut in the same manner. Thoreticallly one shoot apex could be multiplied more than one million shoots by repeating shoot sectioning for one year. Culture temperature and light condition were 25C and 4000 lux., respectively. Several herbaceous ornamentals were successfully propagated with the above methods. Roots were obtained on MS medium containing 0,0.1 and 1 ppm IBA after 3-4 week culture except Centaurea for which hyponex nedium was better than MS medium. Rooted plants were taken out from test tubes and acclimatized at 20C and 4000 lux light conditions for 2-3 weeks. They were then planted in the soil in the greenhouse. Seventy to nighty plants started growth and most of plants flowere normally. Thus three concentrations (0,0.1 and 1 ppm) test of BA and IBA were very effective because they covered optimum growth conditions for most of herbaceous plants. In conclusion herbaceous plants could be effectively propagated by the node and shoot split culture by our method.

草本植物的大量繁殖是利用节组织进行的，因为节组织具有产生腋芽和不定芽的能力。所得方法简便易行。即茎尖在含有0、0.1和1 ppm BA的MS培养基上培养2-3周。然后，如果茎不伸长，则将具有节的茎横向切割或垂直切割。将切下的片在相同的培养基上传代培养，2-3周后，以相同的方式切割。经一年多次的茎尖切片，一个茎尖可增殖100万株以上。培养温度和光照条件为25 ℃，4000勒克斯，分别用上述方法成功地繁殖了几种草本植物。除矢车菊外，其它3种培养基均能诱导出根，其中以Hyponex nedium的效果较好。将生根的植物从试管中取出，并在20 ℃和4000勒克斯光照条件下驯化2-3周。然后将它们种植在温室的土壤中。70 ~ 100株开始生长，大部分植株正常开花。因此，BA和IBA的3个浓度（0、0.1和1 ppm）试验是非常有效的，因为它们涵盖了大多数草本植物的最佳生长条件。因此，采用节段和茎段离体培养方法可以有效地繁殖草本植物。