ADVENTITIOUS BUD FORMATION FROM INTERCALARY MERISTEM AND MASS PROPAGATION OF HERBACEOUS PLANTS.

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Mass propagationof of herbaceous plants was coducted using nodal tissue because the tissue has capacity to produce axillary and adventitious buds. The methology btained here was very simple and convinient. Namely, shoot apices was cultured on MS medium containing 0,0.1 and 1 ppm BA for 2-3 weeks. Then stem with nodes was traversely cut or vertically if stem did not elongate. The cut pieces were subcultured on the same media and after 2-3 weeks, they were cut in the same manner. Thoreticallly one shoot apex could be multiplied more than one million shoots by repeating shoot sectioning for one year. Culture temperature and light condition were 25C and 4000 lux., respectively. Several herbaceous ornamentals were successfully propagated with the above methods. Roots were obtained on MS medium containing 0,0.1 and 1 ppm IBA after 3-4 week culture except Centaurea for which hyponex nedium was better than MS medium. Rooted plants were taken out from test tubes and acclimatized at 20C and 4000 lux light conditions for 2-3 weeks. They were then planted in the soil in the greenhouse. Seventy to nighty plants started growth and most of plants flowere normally. Thus three concentrations (0,0.1 and 1 ppm) test of BA and IBA were very effective because they covered optimum growth conditions for most of herbaceous plants. In conclusion herbaceous plants could be effectively propagated by the node and shoot split culture by our method.