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STRUCTURE OF ACTIVE SITE REGIONS ON EXO-GLYCOSIDASES

STRUCTURE OF ACTIVE SITE REGIONS ON EXO-GLYCOSIDASES
外切糖苷酶活性位点区域的结构
批准号:
07660087
负责人:
MATSUI Hirokazu
金额:
$1.34万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
1995
资助国家:
日本
项目状态:
已结题
起止时间:
1995 至 1996

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中文摘要
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英文摘要
Sugar beet alpha-glucosidase was modified with CBE.An analysis of an CBE-labeled peptide isolated from the digest of modified enzyme showed that the sequence around the essential amino acid was -DGIWIDMNE-. A cDNA encoding sugar beet alpha-glucosidase was cloned from a library constructed from mRNA of suspension cultured cells. The cDNA had an open reading frame encoding a polypeptide of 913 amino acid residues. The deduced amno acid sequence indicated relatively high homology in the range of 28.2 to 54.3% to those for other alpha-glucosidases.The gene encoding isomaltotrio-dextranase was cloned from Brevibacterium fuscum, and analyzed. The deduced amno acid sequence (641 residues) had 80% of homology to that of Arthrobacter dextranase.
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通讯作者:
S.Iwanami: "Chemical Modification and Amino Acid Sequence of Active Site in Sugar Beet α-Glucosidase" Biosci.Biotech.Biochem.,. 59. 459-463 (1995)
S.Iwanami:“甜菜 α-葡萄糖苷酶活性位点的化学修饰和氨基酸序列”Biosci.Biotech.Biochem., 59. 459-463 (1995)
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发表时间:
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通讯作者:
T.Mizuno: "Reinvestigation of Isomaltotrio-dextranase from Brevibacterium fuscum var.dextranlyticum" J.Appl.Glycosci. 43. 357-363 (1996)
T.Mizuno:“对来自短杆菌变种葡聚糖的异麦芽三葡聚糖酶的重新研究”J.Appl.Glycosci。
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S.Onodera: "Molecular Cloning and Nucleotide Sequences of cDNA and Gene Encoding endo-Inulinase from Penicillium purpurogenum" Biosci.Biotech.Biochem.60. 1780-1785 (1996)
S.Onodera:“来自紫青霉的 cDNA 和编码内切菊粉酶的基因的分子克隆和核苷酸序列”Biosci.Biotech.Biochem.60。
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24
    The direct method foreign language learning by acting of actual 3-D robots on 2-D projection
    • 批准号:
      16K12788
    • 项目类别:
      Grant-in-Aid for Challenging Exploratory Research
    • 资助金额:
      $2.08万
    • 财政年份:
      2016
    • 负责人:
      MATSUI Hirokazu
    • 依托单位:
    Identification and functional analysis of novel metabolizing enzymes of plant hormones
    • 批准号:
      24580134
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $3.49万
    • 财政年份:
      2012
    • 负责人:
      MATSUI Hirokazu
    • 依托单位:
    Foreign language conversation learning through the direct method by robot teachers
    • 批准号:
      24650553
    • 项目类别:
      Grant-in-Aid for Challenging Exploratory Research
    • 资助金额:
      $1.58万
    • 财政年份:
      2012
    • 负责人:
      MATSUI Hirokazu
    • 依托单位:
    studies on New Reactions in Glycosylase Catalysis
    • 批准号:
      02806014
    • 项目类别:
      Grant-in-Aid for General Scientific Research (C)
    • 资助金额:
      $1.09万
    • 财政年份:
      1990
    • 负责人:
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    • 依托单位:
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