STRUCTURE OF ACTIVE SITE REGIONS ON EXO-GLYCOSIDASES
STRUCTURE OF ACTIVE SITE REGIONS ON EXO-GLYCOSIDASES
批准号:
07660087
负责人:
MATSUI Hirokazu
金额:
$1.34万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
1995
资助国家:
日本
项目状态:
已结题
起止时间:
1995 至 1996
中文摘要
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英文摘要
Sugar beet alpha-glucosidase was modified with CBE.An analysis of an CBE-labeled peptide isolated from the digest of modified enzyme showed that the sequence around the essential amino acid was -DGIWIDMNE-. A cDNA encoding sugar beet alpha-glucosidase was cloned from a library constructed from mRNA of suspension cultured cells. The cDNA had an open reading frame encoding a polypeptide of 913 amino acid residues. The deduced amno acid sequence indicated relatively high homology in the range of 28.2 to 54.3% to those for other alpha-glucosidases.The gene encoding isomaltotrio-dextranase was cloned from Brevibacterium fuscum, and analyzed. The deduced amno acid sequence (641 residues) had 80% of homology to that of Arthrobacter dextranase.
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A. Kimura:“黑曲霉 α-葡萄糖活性位点的鉴定”Biosci., 3(出版中)。
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S.Iwanami: "Chemical Modification and Amino Acid Sequence of Active Site in Sugar Beet α-Glucosidase" Biosci.Biotech.Biochem.,. 59. 459-463 (1995)
S.Iwanami:“甜菜 α-葡萄糖苷酶活性位点的化学修饰和氨基酸序列”Biosci.Biotech.Biochem., 59. 459-463 (1995)
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T.Mizuno: "Reinvestigation of Isomaltotrio-dextranase from Brevibacterium fuscum var.dextranlyticum" J.Appl.Glycosci. 43. 357-363 (1996)
T.Mizuno:“对来自短杆菌变种葡聚糖的异麦芽三葡聚糖酶的重新研究”J.Appl.Glycosci。
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S.Onodera: "Molecular Cloning and Nucleotide Sequences of cDNA and Gene Encoding endo-Inulinase from Penicillium purpurogenum" Biosci.Biotech.Biochem.60. 1780-1785 (1996)
S.Onodera:“来自紫青霉的 cDNA 和编码内切菊粉酶的基因的分子克隆和核苷酸序列”Biosci.Biotech.Biochem.60。
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H.Matsui: "Cloning and Sequencing of a cDNA Encoding alpha-Glucosidase from Sugar Beet" Biosci.Biotech.Biochem.61, (in press). (1997)
H.Matsui:“编码甜菜 α-葡萄糖苷酶的 cDNA 的克隆和测序”Biosci.Biotech.Biochem.61,(印刷中)。
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共 24 条
The direct method foreign language learning by acting of actual 3-D robots on 2-D projection
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财政年份:2016
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财政年份:2012
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负责人:MATSUI Hirokazu
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依托单位:
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财政年份:2012
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studies on New Reactions in Glycosylase Catalysis
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负责人:MATSUI Hirokazu
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依托单位:
海外基金