Observed Cloning Gene on Mitotic Chromosomes at that number in Basidiomycetes

在担子菌中有丝分裂染色体上观察到的该数量的克隆基因

• 批准号: 07660206
• 负责人: MIURA Kiyoshi
• 金额: $ 1.47万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660206/
• 关键词: pulsed-field gel electrophoresis; confocal laser scan microscope; DAPI; basidiomycetes; chromosome; monokaryon; nuclear; dikaryon; プロトプラスト; 共焦点レーザ走査顕微鏡; パルスフィールド; エノキタケ; PCR; プローブ; ハイブリダイゼーション

The karyotype of Flammulina velutipes (Curt : Fr.) Sing was analyzed electrophoretically by contour-clamped homogeneous electric field (CHEF) gel electrophoresis and hydridization with DNA probe.The chromosomal DNA from the monokaryon (Fv-4K) and the dikaryon (Fv-4) were resolved into six and eight bands, respectively.The sizes of the chromosomes were ranged from 1.9 to 6.0 megabase pairs. Each of the separated bands of chromosomal DNA was identified and was assigned by use of five cloned probes.The number of these chromosomes was estimated to be six and twelve, respectively, and the size of the entire genome was estimated to be about 20.1 Mb and 38.6 Mb, respectively.From comparison of patterns, and hybridization, we deduced the existence of allelic chromosomes of different sizes in the Fv-4 strain. Mitotic chromosomes of Pleurotus cornucopiae (Paulet) Rolland var. citrinopileatus by the Basidium burst method were stained with the fluoresent dye 4', 6-diamidino-2-phenylindole (DAPI), but the component of chromosomes were not clearly distingushed. because chromosomes were very small specimens.

本文报道了金针菇（Flammulina velutipes（Curt：Fr.）采用恒切均相电场（CHEF）凝胶电泳和DNA探针杂交技术对Sing进行了分子生物学分析，结果表明，单核体（Fv-4K）和双核体（Fv-4）的染色体DNA分别被分离成6条和8条带，染色体大小为1.9 ~ 6.0兆碱基对。用5个克隆探针对染色体DNA的每条带进行了鉴定和定位，估计染色体数目分别为6条和12条，整个基因组的大小分别为20.1Mb和38.6Mb。我们推测Fv-4株中存在大小不等的等位染色体。有丝分裂染色体的研究。用荧光染料4 '，6-二脒基-2-苯基吲哚（DAPI）对用Basidium burst法分离的金顶侧耳（Citrinopileatus）染色体进行染色，但不能清楚地区分染色体的组成。因为染色体是非常小的样本。