High resolution inverted confocal laser-scan microscope with FCS

带 FCS 的高分辨率倒置共焦激光扫描显微镜

• 批准号: 449446481
• 金额: --
• 依托单位: Universität Stuttgart
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/449446481?language=en
• 关键词: resolution; inverted; confocal; laser; scan

This proposal aims at the akquisiton of a high-resolution confocal laser-scanning-microscope, fast acquisition mode, large incubation unit and a module for measurements by fluorescence correlation spectroscopy is applied for. A large incubation unit will ensure physiological conditions for long-term experiments using living cells. The FCS module will allow to measure protein-protein interactions, to perform co-localization studies and to determine intracellular effective diffusion constants. The envisaged instrument additionally covers needs for conventional confocal microscopic measurements that can be performed by point-scanning microscopy and will therefore also contribute to reducing waiting times for the presently available confocal system.The requested microscope will be operated centrally in the technology platform “Cellular Analytics” of the Stuttgart Research Center Systems Biology and is therefore open to all members and associated workgroups across the university. The time of usage for the instrument is calculated as 2600 hours within the first year.

本方案针对高分辨率共聚焦激光扫描显微镜的研制，采用快速采集模式、大型孵化装置和荧光相关光谱测量模块。一个大型的培养单元将确保使用活细胞进行长期实验的生理条件。FCS模块将允许测量蛋白质之间的相互作用，执行共定位研究，并确定细胞内的有效扩散常数。设想的仪器还包括可由点扫描显微镜执行的传统共聚焦显微镜测量的需求，因此还将有助于减少目前可用的共聚焦系统的等待时间。所需的显微镜将在斯图加特研究中心系统生物学的技术平台“细胞分析”中集中操作，因此向大学所有成员和相关工作组开放。该仪器的使用时间按第一年内的2600小时计算。