High resolution inverted confocal laser-scan microscope with FCS

带 FCS 的高分辨率倒置共焦激光扫描显微镜

• 批准号: 449446481
• 金额: --
• 依托单位: Universität Stuttgart
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/449446481?language=en
• 关键词: resolution; inverted; confocal; laser; scan

This proposal aims at the akquisiton of a high-resolution confocal laser-scanning-microscope, fast acquisition mode, large incubation unit and a module for measurements by fluorescence correlation spectroscopy is applied for. A large incubation unit will ensure physiological conditions for long-term experiments using living cells. The FCS module will allow to measure protein-protein interactions, to perform co-localization studies and to determine intracellular effective diffusion constants. The envisaged instrument additionally covers needs for conventional confocal microscopic measurements that can be performed by point-scanning microscopy and will therefore also contribute to reducing waiting times for the presently available confocal system.The requested microscope will be operated centrally in the technology platform “Cellular Analytics” of the Stuttgart Research Center Systems Biology and is therefore open to all members and associated workgroups across the university. The time of usage for the instrument is calculated as 2600 hours within the first year.

该提案旨在使用高分辨率共聚焦激光扫描 - 微镜，快速采集模式，大孵育单元和通过荧光相关光谱测量的模块进行的Akquisiton。大型孵化单元将确保使用活细胞进行长期实验的身体状况。 FCS模块将允许测量蛋白质 - 蛋白质相互作用，进行共定位研究并确定细胞内有效扩散常数。所设想的仪器还涵盖了可以通过点扫描显微镜执行的常规共聚焦显微镜测量的需求，因此还将有助于减少目前可用的共聚焦系统的等待时间。因此，所请求的显微镜将在STUTTGART研究中心系统生物学和相关的工作中，并将其与所有成员开放。仪器使用时间在第一年内为2600小时。