High resolution inverted confocal laser-scan microscope with FCS

本课题旨在研制高分辨率共聚焦激光扫描显微镜，采用快速采集模式、大培养单元和荧光相关光谱测量模块。一个大的孵育单元将确保使用活细胞进行长期实验的生理条件。FCS模块将允许测量蛋白质相互作用，进行共定位研究，并确定细胞内有效扩散常数。设想的仪器还满足了可通过点扫描显微镜进行的传统共聚焦显微测量的需求，因此也将有助于减少目前可用的共聚焦系统的等待时间。所要求的显微镜将在斯图加特研究中心系统生物学的技术平台“细胞分析”中集中操作，因此对整个大学的所有成员和相关工作组开放。仪器第一年的使用时间按2600小时计算。

This proposal aims at the akquisiton of a high-resolution confocal laser-scanning-microscope, fast acquisition mode, large incubation unit and a module for measurements by fluorescence correlation spectroscopy is applied for. A large incubation unit will ensure physiological conditions for long-term experiments using living cells. The FCS module will allow to measure protein-protein interactions, to perform co-localization studies and to determine intracellular effective diffusion constants. The envisaged instrument additionally covers needs for conventional confocal microscopic measurements that can be performed by point-scanning microscopy and will therefore also contribute to reducing waiting times for the presently available confocal system.The requested microscope will be operated centrally in the technology platform “Cellular Analytics” of the Stuttgart Research Center Systems Biology and is therefore open to all members and associated workgroups across the university. The time of usage for the instrument is calculated as 2600 hours within the first year.