Role of protein tyrosine kinase in functional changes of hyperplastic arteries
蛋白酪氨酸激酶在增生性动脉功能变化中的作用
基本信息
- 批准号:07660404
- 负责人:
- 金额:$ 1.41万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1995
- 资助国家:日本
- 起止时间:1995 至 1996
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We investigated how protein kinases were involved in functional changes in two kinds of experimentally made hyperplastic arteries. Vascular angioplasty was performed by rubbing the endothelial layr of rabbit carotid artery with a balloon catheter. At 6 weeks of surgery, marked hyperplasia in the intimal layr of the artery was observed. Myosin light chain (MLC) phosphorylation and the contractile response to prostaglandin F2alpha (PGF2alpha) were enhanced in the artery, although the cytosolic Ca^<2+> mobilization was similar to control artery. While serine/threonine kinase inhibitors inhibited the MLC phosphorylation and the contraction in hyperplastic artery than in normal one, tyrosine kinase inhibitors equally inhibited the contraction in both arteries, suggesting that tyrosine kinase was not responsible for the hypercontraction. In a separate experiment, rats were subcutaneously given monocrotaline, which impaired the endothelium of pulmonary artery, resulting in pulmonary hypertens … More ion. Monocrotaline-affected artery showed a marked hyperplasty in the medial layr. In this artery, the relaxing action of the endothelium was markedly impaired. The artery also produced an active tension at the resting state. This active tension was probably due to the membrane depolarization and increased cytosolic Ca^<2+>. The endothelium of the artery seemed to work to increase the resting tension. At some stage, the contractile response to PGF2alpha was enhanced. In this case, tyrosine kinase inhibitors exerted more potent inhibition on the contraction than in the control artery. Thus, tyrosine kinase may be responsible for the abnormal contractility in this type of vascular disease.In addition to the above study we evaluated the selectivity of several protein kinase inhibitors and found that a tyrosine kinase may be involved in PGF2alpha-induced activation of voltage-dependent Ca^<2+> channels in rabbit aorta. We also investigated the mechanism of protein kinase C (PKC)-dependent Ca^<2+> sensitization of contraction. It was suggested that PKC activation and the subsequent release of arachidonic acid inhibit MLC phosphatase, increasing the level of MLC phosphorylation. Tyrosine kinase does not seem to be present in the down-stream of PKC activation. Less
我们研究了蛋白激酶如何参与两种实验性增生动脉的功能变化。用球囊导管摩擦兔颈动脉内皮层进行血管成形术。在手术6周时,观察到动脉内膜层明显增生。动脉中肌球蛋白轻链(MLC)磷酸化和对前列腺素F2 α(PGF 2 α)的收缩反应增强,尽管胞浆Ca^2+动员与对照动脉相似。丝氨酸/苏氨酸激酶抑制剂抑制MLC磷酸化和正常动脉的收缩,酪氨酸激酶抑制剂同样抑制在两个动脉的收缩,表明酪氨酸激酶不是负责过度收缩。在另一项实验中,大鼠皮下注射野百合碱,损伤肺动脉内皮,导致肺动脉高压 ...更多信息 离子。野百合碱作用的动脉在内侧层表现出明显的过度成形。在这条动脉中,内皮的舒张作用明显受损。动脉在静息状态下也产生主动张力。这种主动张力可能是由于膜去极化和胞浆Ca^<2+>增加所致。动脉内皮似乎起着增加静息张力的作用。在某些阶段,对PGF 2 α的收缩反应增强。在这种情况下,酪氨酸激酶抑制剂对收缩的抑制作用比对照动脉更强。除上述研究外,我们还评估了几种蛋白激酶抑制剂的选择性,发现酪氨酸激酶可能参与PGF 2 α诱导的兔主动脉电压依赖性Ca^<2+>通道的激活。我们还研究了蛋白激酶C(PKC)依赖的Ca^<2+>敏感性收缩的机制。这表明,PKC激活和随后的花生四烯酸释放抑制MLC磷酸酶,增加MLC磷酸化水平。酪氨酸激酶似乎不存在于PKC激活的下游。少
项目成果
期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Asano,M.,Matsunaga,K.,Ito,K.et al.: "Selectivity of action staurosporine on Ca^<2+> movements and contractions in vascular smooth muscles" Eur.J.Pharmacol.294(2/3). 693-701 (1995)
Asano,M.,Matsunaga,K.,Ito,K.等人:“星形孢菌素对血管平滑肌 Ca^2 运动和收缩的选择性”Eur.J.Pharmacol.294(2/3)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Asano,M.,Ito,K.et al.: "Selectivity of action of staurosporine on Ca^<2+> movements and contractions in vascular smooth muscles" Eur.J.Pharmacol.294. 693-701 (1995)
Asano,M.,Ito,K.等人:“星形孢菌素对血管平滑肌中 Ca^2 运动和收缩的选择性作用”Eur.J.Pharmacol.294。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Asano, M.et al.: "Selectivity of action of staurosporine on Ca^<2+> movements and contractions in vascular smooth muscles" Eur.J.Pharmacol. 294(2/3). 693-701 (1995)
Asano, M.等人:“星形孢菌素对血管平滑肌中 Ca^2 运动和收缩的选择性作用”Eur.J.Pharmacol。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Seto, M.et al.: "Selective inhibition of myosin phosphorylation and tension of hyperplastic arteries by the kinase inhibitor HA1077" Eur.J.Pharmacol. 276(1). 27-33 (1995)
Seto, M.et al.:“激酶抑制剂 HA1077 对肌球蛋白磷酸化和增生动脉张力的选择性抑制”Eur.J.Pharmacol。
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- 发表时间:
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- 影响因子:0
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- 通讯作者:
Seto,K.,Shindo,K.,Ito,K.,et al.: "Selective inhibition of myosin phosphorylation and tension of hyperplastic arteries by the kinase inhibitor HA1077" Eur.J.Pharmacol.276(1). 27-33 (1995)
Seto,K.、Shindo,K.、Ito,K. 等人:“激酶抑制剂 HA1077 对肌球蛋白磷酸化和增生动脉张力的选择性抑制”Eur.J.Pharmacol.276(1)。
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ITO Katsuaki其他文献
ITO Katsuaki的其他文献
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The role of P2X receptor in overactive bladder and application of drugs targeting the receptor
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21580365 - 财政年份:2009
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Significance of cross-talk among ADP, thromboxane A2 and collagen during collagen-induced thrombus formation
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15580261 - 财政年份:2003
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Characteristics of aggregation of bovine platelets and clarification of molecular mechanism responsible for a genetic hemorrhagic disease in cattle
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12660272 - 财政年份:2000
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Biological significance of palytoxin-sensitive ion channel associated with NaィイD1+ィエD1,KィイD1+ィエD1-ATPase molecule
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09460140 - 财政年份:1997
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Grant-in-Aid for Scientific Research (B)
The role of plasmalemmal Ca^<2+> channels and intracellular Ca^<2+> stores in vascular smooth muscles during the development of vascular resistance
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04660325 - 财政年份:1992
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Cellular calcium movements and the role in regulating contraction and relaxation of vascular smooth muscles of resistance vessels
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02660312 - 财政年份:1990
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Studies on the alterations of lung endothelial cells and the metabolism of autacoids during lung diseases
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60480095 - 财政年份:1985
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