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Electron microsopic and histochemical study on the neural circuitry for color vision in the monkey retina

猴视网膜色觉神经回路的电子显微镜和组织化学研究

基本信息

批准号：
07670042
负责人：
TSUKAMOTO Yoshihiko
金额：
$ 0.19万
依托单位：
Hyogo College of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670042/
关键词：
monkey primate retina foveal center electron microscopy cone photoreceptor 網膜神経回路

项目摘要

1) Synaptic connections between cone photoreceptor terminals and bipolar cell dendrites were analyzed on a series of EM photographs of serial sections from Macaca fuscicularis. ON-type bipolar cells had been thought to have solely invaginating junctions with cone pedicles but were revealed to have basal junctions as well.2) We reconstructed 3D-structures of H1 and H2 type horizontal cell to examine how horizontal cells are involved in the circuitry for color vision. The H1 type cell has about 40 synapses with 7 to 8 cones classified both ret and green. The H2 type cell receives input not only from red and green cones but also from blue cones in a relatively wide receptive field.3) The axons and synaptic terminals of both cone and rod photoreceptors at the eccentricity of 3.5mm in the retina of japanese monkey, Macaca fuscata, were compared in morphology. There were many differences between cones and rods in morphological respects which relate with electrical and chemical signal transmi … More ssion.4) We executed immunohistochemical staining of synaptic vesicles using anti-synaptophysin to exmine their axonal flow. The materials of japanese monkey retinas were obtained from Primate Research Institute, Kyoto University. Synaptophysin-positive processes were successfully visualized at light microsopic level.5) Since we obtained exremely well fixed retina tissues, we observed the foveal center by electron microscopy. A most foveal cone was found at the base of the fovea. We have been photographing this special cone to examine its synaptic divergence.6) The plan we proposed to label red cone photoreceptors by enzymatic histochemistry using NTB had depended on an article published in Science in 1997. However, the reproducibility of this methodology was poor. The reason may be due to yet undknown but recently enlightened mechanisms which relate with NO,whose existence has been proved in retinas. Since NTB is used for visualizing NO activity, the previous results using NTB may be associated with NO which had not been known at that time. We have taken care of the methodology in this project term. Less

1)用透射电镜观察了黑尾猕猴（Macacafuscicularis）视锥细胞终末与双极细胞树突之间的突触联系。ON型双极细胞被认为只具有与锥体蒂的内陷连接，但也被发现具有基底连接。2）我们重建了H1和H2型水平细胞的三维结构，以研究水平细胞如何参与色觉回路。H1型细胞具有约40个突触，具有7至8个分为ret和绿色的视锥。H_2型细胞不仅接受红、绿色视锥细胞的传入，而且接受蓝视锥细胞的传入，感受野较宽。3）对日本猕猴视网膜偏心距为3.5mm处的视锥细胞和视杆细胞的轴突和突触末梢进行了形态学比较。视锥细胞和视杆细胞在形态学方面存在许多差异，这些差异与细胞内的电和化学信号传递有关。 ...更多信息 ssion.4）用抗突触素对突触囊泡进行免疫组织化学染色，观察其轴突流。日本猴视网膜材料购自京都大学灵长类研究所。5）由于我们获得了固定良好的视网膜组织，我们通过电子显微镜观察了中心凹中心。在中心凹底部发现一个最大的中心凹圆锥体。我们一直在拍摄这种特殊的锥细胞，以检查其突触分歧。6）我们提出的使用NTB通过酶组织化学标记红锥光感受器的计划依赖于1997年发表在《科学》杂志上的一篇文章。然而，这种方法的重现性差。其原因可能是由于尚未了解，但最近启发的机制，与NO，其存在已被证明在视网膜。由于NTB用于可视化NO活性，因此先前使用NTB的结果可能与当时未知的NO有关。我们已经注意到了这个项目期间的方法。少