Quantitative analysis of nm23 gene product in human cancers

应用免疫印迹技术结合光密度法和免疫组化技术，对人正常组织、肺癌组织、子宫内膜癌组织中nm 23蛋白的总量、H1和H2亚型的相对比值（H2/H1）进行了研究。包括癌组织在内的所有组织都含有两种亚型，20.5kD为H1，18 kD为H2亚型。在正常组织中，总nm 23蛋白在肝、肾和肾上腺中的表达水平较高，而在心脏和肌肉组织中的表达水平较低。H2水平高于H1水平在所有组织中，检查，其H2/H1的比例是不同的组织。免疫组化显示肝、胰腺和肾上腺组织中实质细胞呈均匀染色，胃粘膜和肾组织中实质细胞呈不均匀染色。另一方面，在肺癌中，癌组织中的H1和H2水平均高于非肿瘤性肺组织，而在肺腺癌和肺鳞癌中，肿瘤H2/H1比值低于非肿瘤性肺组织。我们的研究表明，nm 23蛋白在肺腺癌中的表达与肿瘤的组织分化程度显著相关，而与转移潜能和肿瘤分期无关。nm 23-H1和H2在子宫内膜癌中的表达与分期、转移、肿瘤大小、肌层浸润、雌激素受体及绝经无关。提示nm 23-H1、-H2的高表达与组织分化程度呈正相关。

The total amount of nm23 protein, relative rations of H1 and H2 isoform (H2/H1) in human normal tissues, lung cancer tissues, endometrial cancer tissues were studied in combination with semiquantitative immunoblot technique followed densitometry and immunohistochemistry using monoclonal antibody against nm23 protein (Pan-242). All tissues including cancer tissues contained both isoform recognized in 20.5 kD as H1 and 18kD as H2 isoforms. In normal tissues, the level of total nm23 protein was higher in liver, kidney and adrenal gland, and lesser in heart and muscle tissues. H2 levels were higher than H1 levels in all tissues, examined, and their H2/H1 rations were varied from tissue to tissue. The immunohistochemistry revealed that the parenchymal cells were homogeneously stained in liver, pancreas and adrenal gland tissues and heterogeneously stained in gastric mucosa and kidney tissues. On the other hand, in lung cancers, both H1 and H2 levels in cancer tissues were higher than non-neoplastic lung tissues, and tumor H2/H1 ratio were lower than non-neoplastic ones in adenocarcinoma and squamous cell carcinoma of the lung. Our study indicate that nm23 protein levels in adenocarcinoma of the lung were significantly correlated with histologic differentiation of tumors, whereas they were not correlated with metastasis potentials and tumor stage. Furthermore, in endometrial cancers, the expression of nm23-H1 and H2 was not correlated with stage, metastasis, tumor size, myometrial invasion, estrogen receptor or menopause. It follows from the findings presented that the high expression o nm23-H1 and -H2 is positively correlated with histological differentiation.