Molecular mechanisms for enhancement of human cytomegalovirus replication by chemokine, interleukin 8

CMV与IL-8（一种趋化性细胞因子）的相关性已由几项观察结果提出。我们研究了CMV对IL-8产生的影响，并分析了CMV诱导THP-1细胞产生IL-8的分子机制。用CMV Towne株感染THP-1细胞。ELISA法检测IL-8的产生，北方印迹法检测IL-8特异性转录本的表达。用荧光素酶报告基因分析法检测CMV感染后IL-8基因转录调控基因的表达。电泳迁移率变动分析（EMSA）检测了与增强子区域结合的核因子。CMV感染诱导THP-1细胞IL-8基因转录，导致IL-8分泌。对IL-8基因的功能分析表明，AP-1和NF-κ B因子结合元件都参与了对CMV的反应性。EMSA证实CMV诱导NF-κ B和AP-1复合物的形成。这项研究表明，CMV通过激活转录因子调节与IL-8产生相关的炎症反应。

The association of CMV with IL-8, a chemotactic cytokine, has been suggested by several observations. We examined the effects of CMV on the production of IL-8 and also analyzed the molecular mechanisms of CMV-induced IL-8 production using THP-1 cells. THP-1 cells were infected with CMV,Towne strain. The production of IL-8 was measured by an ELISA.IL-8 specific transcript was detected by Northern blot. The transcriptional regulatory gene expression of IL-8 gene in response to CMV infection was measured by luciferase reporter gene assay. The nuclear factors which bind to the enhancer regions were also examined by electrophoretic mobility shift assay (EMSA). Infection with CMV induced IL-8 gene transcription in THP-1 cells, leading to IL-8 secretion. The functional analysis on the IL-8 gene revealed that both AP-1 and NF-kB factor binding elements were involved in conferring the responsiveness to CMV.Moreover, EMSA demonstrated that CMV induced the formation of NF-kB and AP-1 complexes. This study suggests that CMV modulates inflammatory responses associated with IL-8 production through activation of transcription factors.