Structure and Function of MHC Class Ib Molecules

MHC Ib 类分子的结构和功能

• 批准号: 07670359
• 负责人: KASAHARA Masanori
• 金额: $ 0.38万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670359/
• 关键词: Major histocompatibility complex; Class Ib molecules; FcRn molecule; Chromosomal duplication; Fcリセプター

Besides the class I genes that are highly polymorphic and encoded within the major histocompatibility complex (MHC), the genome of mammals contains class I genes that are oligo- or monomorphic. The latter category of class I genes, known as class Ib genes, encodes the neonatal intestinal Fc receptor (FcRn) involved in the transport of IgG from mother to offspring ; CD1 molecules involved in the presentation of glycolipids to T cells ; and zinc-alpha2-glycoprotein with no known function. The aims of the present study were to gain insights into the structure, function, and evolution of class Ib genes. The specific results obtained are as follows : 1) We cloned the genomic clones coding for the heavy chains of the human and mouse FcRn molecules, and determined the chromosomal localization of the genes. 2) We showed that the genetic region where the CD1 genes have been mapped is paralogous to the MHC.3) We showed that the mouse gene for zinc-alpha2-glycoprotein maps outside the MHC.4) We found that human CD1b molecules probably have no bound peptides.

除了在主要组织相容性复合体(MHC)内编码的高度多态的I类基因外，哺乳动物的基因组还包含寡核苷酸或单态的I类基因。后一类I类基因，称为Ib类基因，编码新生儿肠道Fc受体(FcRN)，参与免疫球蛋白从母亲到后代的运输；CD1分子，参与糖脂向T细胞的呈递；以及锌-α2-糖蛋白，功能未知。本研究的目的是深入了解Ib类基因的结构、功能和进化。获得的具体结果如下：1)克隆了人和小鼠FcRN分子重链的基因组克隆，并对基因进行了染色体定位。2)我们发现CD1基因被定位的遗传区域与MHC.3是平行的)我们发现小鼠锌-α2-糖蛋白基因定位在MHC.4之外)我们发现人类CD1b分子可能没有结合的多肽。

项目成果

Kasahara, M.: "Mapping of acidic epididymal glycoprotein (Aeg) genes to mouse chromosome 17." Mammalian Genome. 6. 52-54 (1995)

Kasahara, M.：“酸性附睾糖蛋白 (Aeg) 基因与小鼠 17 号染色体的映射。”

Kobari, F.: "Exon-intron organization of Xenopus MHC class II β-chain genes." Immunogenetics. 42. 376-385 (1995)

Kobari, F.：“非洲爪蟾 MHC II 类 β 链基因的外显子-内含子组织。” 免疫遗传学 42. 376-385 (1995)

Kasahara, M.: "Origin and evolution of the class I gene family : Why are some of the mammalian class I genes encoded outside the major histocompatibility complex? Rebuttal to the commentary by Drs. Ahouse and Simister." Res. Immunol.147. 353 (1996)

Kasahara, M.：“I 类基因家族的起源和进化：为什么一些哺乳动物 I 类基因在主要组织相容性复合体之外编码？反驳 Ahouse 和 Simister 博士的评论。”

Hayashi, M.: "The mouse genes encoding the third pair of beta-type proteasome subunits regulated reciprocally by IFN-gamma : Structural comparison, chromosomal localization, and analysis of the promoter." J.Immunol. (in press).

Hayashi, M.：“编码由 IFN-gamma 相互调节的第三对 β 型蛋白酶体亚基的小鼠基因：结构比较、染色体定位和启动子分析。”

Kandil, E.: "The human gene encoding the heavy chain of the major histocompatibility complex class I-like Fc receptor (FCGRT) maps to 19q13. 3." Cytogenet. Cell Genet.73. 97-98 (1996)

Kandil, E.：“编码主要组织相容性复合物 I 类 Fc 受体 (FCGRT) 重链的人类基因映射到 19q13.3。”