MOLECULAR EPIDEMIOLOGY OF APOLIPOPROTEIN E AND VITAMIN D RECEPTOR IN NORTHERN PART OF KYOTO PREFECTURE

京都府北部地区载脂蛋白E和维生素D受体的分子流行病学

• 批准号: 07670449
• 负责人: MIZUNO Toshiki
• 金额: $ 1.34万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670449/
• 关键词: Dementia of Alzheimer type; Apolipoprotein E(ApoE); molecular epidemiology; アポリポタンパクE; アポリポ蛋白E(ApoE)

The PCR-RFLP method is widely used for detecting genotypes of Apolipoprotein E (ApoE). However, there are some problems to detect the heterozygous pattern of ApoE by PCR-RFLP method. When the most popularly used PCR primers were employed, the density of restriction fragments vary depending on genotypes of the gene. Also, there are some problems to detect heterozygous pattern of ApoE by direct sequencing with PCR.We prepared new PCR primers for detecting genotypes of ApoE.After amplification by PCR,genotypes of Apolipoprotein were determined by RFLP method and direct sequencing. When the new primers were used, density of fragment after digestion by restriction enzyme was identical and heterozygous pattern was easily identified by direct sequencing. These results indicated that the new primers were more reliable for detecting genotypes of ApoE than ordinal primers.We performed the genotype analysis of ApoE in the northern part of Kyoto Prefecture using these new primers for ApoE.Subjects were 198 residents 65 years old and older in the town, 21 eldely in a hospital. These 219 persons were divided into three groups ; no dementia group 183, senile dementia of Alzheimer type (SDAT) group 22 and vascular dementia group 14. The frequencies of ApoE2/2,3/3,2/4,3/4,4/4, were 1.1%, 5.5%, 75.4%, 1.1%, 16.4%, 0.5% in no dementia group and 0%, 0%, 77.2%, 4.5%, 18.2%, 0% in SDAT group. The frequencies of epsilon 4 were 9.3% in no dementia group and 11.4% in SDAT group. The frequency of epsilon 4 was higher in SDAT group than no dementia group, but this difference was not statistically significant. The frequency epsilon 4 in no dementia group in this area was higher reported so far from other Japanese areas, but this difference was not significant, either.

PCR-RFLP方法是目前广泛应用于载脂蛋白E（ApoE）基因型检测的方法。然而，PCR-RFLP方法检测ApoE基因杂合型存在一些问题。当使用最常用的PCR引物时，限制性片段的密度根据基因的基因型而变化。同时，PCR直接测序法检测ApoE杂合子型存在一定的问题，为此，我们设计了新的ApoE基因型PCR引物，经PCR扩增后，用RFLP法和直接测序法检测载脂蛋白基因型。当使用新的引物时，酶切后的片段密度相同，并且通过直接测序容易鉴定杂合模式。本研究应用新引物对京都府北方地区198名65岁以上居民和21名住院老年人的ApoE基因型进行了分析。这219人被分为三组：无痴呆组183人，阿尔茨海默型老年性痴呆（SDAT）组22人和血管性痴呆组14人。非痴呆组ApoE 2/2、3/3、2/4、3/4、4/4的频率分别为1.1%、5.5%、75.4%、1.1%、16.4%、0.5%;非痴呆组和SDAT组中的α 4基因频率分别为9.3%和11.4%。SDAT组的AD基因频率高于非痴呆组，但差异无统计学意义。该地区的非痴呆组中的频率104比日本其他地区高，但这种差异也不显著。

项目成果

T.Mizuno et al: "ApolipoproteinE polymorphism in a local areaof Kyoto prefecture in Japan" Neurobiogy of aging. 17. S124- (1996)

T.Mizuno等人：“日本京都府局部地区的载脂蛋白E多态性”衰老的神经生物学。

T.Mizuno, T.Hashimoto-Gotoh, K Nakajima et al.: "Apolipoprotein E polymorphism in a local area of Kyoto prefecture in Japan." Neurobiology of aging. 17. S124 (1996)

T.Mizuno、T.Hashimoto-Gotoh、K Nakajima 等：“日本京都府局部地区的载脂蛋白 E 多态性”。

H Honjo, M Urabe, K Iwasa, T Okubo, H Tsuchiya, N Kokuchi, T Yamamoto, S Fushiki, T Mizuno, K Nakajima, M Hayashi, and K Hayashi: "Estrogen treatment for senile dementia-Alzheimer's type." Progress in the management of the menopause : Edited by BG Wren.Th

H Honjo、M Urabe、K Iwasa、T Okubo、H Tsuchiya、N Kokuchi、T Yamamoto、S Fushiki、T Mizuno、K Nakajima、M Hayashi 和 K Hayashi：“老年性痴呆 - 阿尔茨海默型痴呆的雌激素治疗。”

T.Mizuno et, al.: "Apolipoprotein E polymorphism in a local area of Kyoto prefecture in Japan" Neurobiology of aging. 17. S124 (1996)

T.Mizuno等人：“日本京都府局部地区的载脂蛋白E多态性”衰老的神经生物学。