THE FORENSIC APPLICATION OF ABO BLOOD GROUP GENES AND BIOLOGICAL SIGNIFICANCE OF THE ANTIGENS

ABO血型基因的法医应用及抗原的生物学意义

• 批准号: 07670488
• 负责人: NISHI Katsuji
• 金额: $ 1.41万
• 依托单位: SHIGA UNIVERSITY OF MEDICAL SCIENCE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670488/
• 关键词: ABO BLOOD GROUP; TRANSFERAZE GENES; TASTE BUDS CELLS; SPERMATOGENESIS; HASSALL'S CORPUSCLES; 糖鎖転移酵素遺伝子; ABO遺伝子; 人獣鑑別

The distribution of the ABO (H) and related antigens in some mammals including human was studied to resolve the biological significance of these aqntigens. Thes antigens were recognized in the male reproductive organs such as epididymidis, prostate and seminal vesicle of human, Japanese monkey, rat, and cat. The ABO related antigens were also expressed in the taste buds cells of the tongue of human, Japanese monkey, rat, dog, rabbit and cat. Form these it is presumed that the ABO and related antigens are associated with the chemoreceptor systems in the taste buds cells and spermatogenesis in the deproductive organs of male mammals. These antigens were additionally observed in the Hassall's corpuscles in the Thymus, showing a role of these antigens in the T-cell educatianal systems of the Thymus. The DNA samples obtained from human, rat, cat, mastomis and fish were ammplified by PCR using the primers prepared for human ABO genotyping. This resulindicate that in the forensic practice, the species identification is important procedure even in ABO genotyping by PCR because forensic material, such as salivaurine and seminal fluid migit be contaminated with the fluid from indoor pets.

本文研究了ABO（H）及其相关抗原在包括人在内的一些哺乳动物中的分布，以阐明这些抗原的生物学意义。这些抗原在人、日本猴、大鼠和猫的雄性生殖器官如附睾、前列腺和精囊中被识别。ABO相关抗原在人、日本猴、大鼠、狗的舌味蕾细胞中也有表达，从这些推测，ABO和相关抗原与味蕾细胞中的化学感受器系统相关，雄性哺乳动物生殖器官的精子发生。这些抗原还观察到的Hassall的小体中的Thursday，显示了这些抗原的作用，在T-细胞educatianal系统的Thursday。用制备的人ABO基因分型引物对人、大鼠、猫、乳鼠和鱼的DNA进行PCR扩增。这一结果表明，在法医学实践中，由于室内宠物体液对法医学材料如唾液、精液等的污染，即使在PCR法ABO基因分型中，种属鉴定也是一个重要环节。

项目成果

Ushiyama Ikuko: "Expression of blood group related carbohydorate antigens in salivary glands and male reproductive organs from rats, cats and humans." Proceedings of 2nd Internet World Congress on Biological Science CD ROM.2. CD ROM (1996)

Ushiyama Ikuko：“大鼠、猫和人类的唾液腺和雄性生殖器官中血型相关的碳水化合物抗原的表达。”

Nishi Katsuji: "Recent developement of blood grouping in the forensic field" The proceedings of the 5th Indo-Pacific Congress on Legal Medicine and Forensic Science. in press (1996)

Nishi Katsuji：“法医学领域血型分析的最新发展”第五届印度-太平洋法律医学和法医学大会的会议记录。

Ushiyama Ikuko: "ABH-related antigens participate in the spermatogenesis of cats and rats and rats." Advances in Forensic Haemogenetics. 6. 449-451 (1996)

牛山郁子：“ABH相关抗原参与猫和大鼠的精子发生。”

Nishi Katsuji: "Recent developments of blood grouping in the forensic field." Proceedings of 5th Indo-Pacific Congress on Legal Medicine and Forensic Science. 5(in press). (1997)

Nishi Katsuji：“法医学领域血型鉴定的最新进展。”

Yamada Mitsuko: "Comparison of the genes for ABO blood group among some mammals and its application to specis identification." The Proceedings of International Symposium Advances in Legal Medicine. 3. (1997)

山田光子：“一些哺乳动物中 ABO 血型基因的比较及其在物种鉴定中的应用。”