Some enzymes in the cerebrospinal fluid and glyco-conjugates may be useful to diagnose brain disorder

脑脊液中的一些酶和糖复合物可能有助于诊断脑部疾病

• 批准号: 15390212
• 负责人: NISHI Katsuji
• 金额: $ 9.54万
• 依托单位: Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390212/
• 关键词: Protease and Protease Inhibitor; CSF; Glycoconjugate; Brain Disorder; シスタチンC; プロスタグランヂンD2; システチンC; プロスタグランヂンD2合成; βトレイス; 睡眠・窒息; 脳虚血; 法医病理学; 脳幹網様体; オリーブ・橋・小脳路; SIDS; レクチン染色; 免疫組織化学染色; 統合失調症; アルツハイマー型痴呆; ドーパミンニューロン

Some enzymes contained in the cerebrospinal fluid (CSF) were purified and antibodies against those enzymes were produced. Puromycin sensitive alanyl-amninopeptidase (PSA) increased its amount in the CSF with post-mortem interval and was detected in senile plaque of the brain with dementia. Cystatin C (CC) acting as a protease inhibitor was clearly detected in the old-infarction area of the brain. Prostaglandin D2 synthase (PGDS), beta-trace, was also detected in the brain bleeding area. These results indicate that PSA, CC and PGDS may be useful in the forensic neuropathology. PGDS known as sleeping control substance was detected in the neurons of cerebellum and medulla oblongata with COX 2, showing the PGDS may relate with posture-regulation during one's sleeping. A large amount of Le X antigen known as stage specific glycoconjugate antigen existed in the hippocampus and the cerebellum. The existence area of the Le X antigen was differ as one's grows, that is, on the neurons in the hippocampus from three-month old-baby, on the astrocytes in the hippocampus from one-year-old baby, and after one-year-old on the astrocytes and micro-glia cells. The carbohydrate depositions were detected in the molecular layer of the dentate gyrus of the hippocampus from patients with memorial disturbance.

对脑脊液（CSF）中的某些酶进行纯化，并产生针对这些酶的抗体。嘌呤霉素敏感的丙氨酰氨肽酶（PSA）在脑脊液中的含量随死后时间的延长而增加，并在痴呆患者的脑老年斑中检测到。在大脑的陈旧梗塞区域中清楚地检测到作为蛋白酶抑制剂的半胱氨酸蛋白酶抑制剂C（CC）。前列腺素D2合酶（PGDS），β-痕量，也检测到脑出血区。提示PSA、CC和PGDS在法医神经病理学中有一定的应用价值。用考克斯2标记法在小脑和延髓神经元中检测到PGDS（睡眠控制物质），提示PGDS可能与睡眠时的姿势调节有关。在海马和小脑中存在大量的LeX抗原，称为阶段特异性糖结合抗原。随着年龄的增长，LeX抗原的存在区域也有所不同，即3个月龄时在海马神经元上，1岁时在海马星形胶质细胞上，1岁以后在星形胶质细胞和小胶质细胞上。在记忆障碍患者海马齿状回分子层中检测到糖沉积。

项目成果

mmuno-histochemical characteristics of necrotic area in the old-infarction area of human brain bu using of antibodies against cystatin c cathepsin B, puromycin sensitive alanyl-aminopeptidase and prostaglandin D2 synthase-A preliminary examination.

采用半胱氨酸蛋白酶抑制剂C组织蛋白酶B抗体、嘌呤霉素敏感丙氨肽酶和前列腺素D2合酶A初步检测人脑陈旧性梗死区坏死区的免疫组织化学特征。

• 发表时间: 2007
• 期刊: Internet Journal of Forensic Medicine and Toxicology 8(In press)
• 作者: A.Kobayashi;I.Takase;T.Kitamura;A.Takamure;M.Ohyagi;A.Morimoto;S.Yamasaki;Y.Yamamoto;I.Ohkubo;K.Nishi;Anil Aggrawa;A.Kobayashi
• 通讯作者: A.Kobayashi

Nishi K.: "Utilization of lectin-histochemistry in forensic neuropathology : lectin staining provide useful information for postmortem diagnosis in forensic neuropathology"Legal Medicine. 5. 117-131 (2003)

Nishi K.：“凝集素组织化学在法医神经病理学中的应用：凝集素染色为法医神经病理学的死后诊断提供有用的信息”法律医学。

Chiba T.: "Inhibition of recombinant dipeptidyl peptidase III by synthetic hemorphin-like peptides"Peptides. 24. 773-778 (2003)

Chiba T.：“合成血吗啡样肽对重组二肽基肽酶 III 的抑制”肽。

Ikemoto K.: "Number of striatal D-neurons is reduced in autopsy brains of schizophrenics"Legal Medicine. 5. S221-S224 (2003)

Ikemoto K.：“精神分裂症患者尸检大脑中纹状体 D 神经元的数量减少”法律医学。

Immuno-histochemical characteristics of necrotic area in the old-infarction area of human brain bu using of antibodies against cystatin c cathepsin B, puromycin sensitive alanyl-aminopeptidase and prostaglandin D2 synthase - A preliminary oxamination.

使用抗胱抑素 C 组织蛋白酶 B、嘌呤霉素敏感丙氨肽酶和前列腺素 D2 合酶抗体对人脑陈旧性梗死区坏死区的免疫组织化学特征进行初步检测。

• 发表时间: 2007
• 期刊: Internet Journal of Forensic Medicine and Toxicology 8(In press)
• 作者: A.Kobayashi;I.Takase;T.Kitamura;A.Takamure;M.Ohyagi;A.Morimoto;S.Yamasaki;Y.Yamamoto;I.Ohkubo;K.Nishi;Anil Aggrawa
• 通讯作者: Anil Aggrawa