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ヒト男性生殖器官ABH関連抗原発現メカニズムの組織化学的、分子生物学的解析

人类男性生殖器官ABH相关抗原表达机制的组织化学和分子生物学分析

基本信息

批准号：
04670353
负责人：
NISHI Katsuji
金额：
$ 1.41万
依托单位：
SHIGA UNIVERSITY OF MEDICAL SCIENCE
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

项目摘要

The ABO blood group antigens were discovered at the bigining of this century. Althogh the biochemical and molecluar-biochemical nature of the ABH and related antigens is now quite well characterized, no clear explanation of the biological significance of these antigens has been proposed. Characterization of cells with regard to their surface and secretory glycoconjugates is important in order to undestand the basis of their physiological and interactive behavior. In this project the expression of the ABH related antigens in mammalian maile reproductive organs, vertebrate organs and the taste buds of some mammals was investigated for the purpose of elucidating the biological significance of the antigens. During the research several informations were obtained, that is,1).Spermatocytes from mammals including the human showed good reactivity with the lectins specific to galactose. Anti A antibody and blood A specific lentins intensively stained epithelial cells of epididumis and spermatozo … More a stored in the epididymis of cats and rats, respectively. Althogh the epithelial cells from human male reproductive organs expressed ABH related antigens corresponding with the blood groups of the donors, AOB,H.Se, Le and X genes are subjected to a tissue - dependent differential expresion.2) The ABH related antigens were reconganized in the taste buds cells of rats, cats, dogs, rabbits, japanese monkeys and human.3) Althogh the ABH antigens were expressed only in secretory cells of fishes, frogs, non primate mammals and new world monkeys, the antigens were expressed in vascular emdothelial cells and sebretory cells of old world monkeys. The antigens was expressed in red blood cells, sebretory cells and vascular emdothelial cells of anthorpoid and human.These results indicate that the ABH related antigens effect the development of spermatpzoa and the function in the taste receptor, and the expression of the antigens is closely related with the vertebrate evolution.The determination methods of ABO-genetypes from fresh blood cells and parafifn embedded tissue were established in orde to execute the research project.The existence of genes encoding ABO-glycosyltransferase in japanese monkey and other mammals has been invesigating in orde to expand this research project. Less

ABO血型抗原是在本世纪初发现的。尽管ABH和相关抗原的生物化学和分子生物化学性质现在已经得到很好的表征，但这些抗原的生物学意义还没有得到明确的解释。细胞表面和分泌糖复合物的表征对于理解其生理和相互作用行为的基础是重要的。本课题研究了ABH相关抗原在哺乳动物雄性生殖器官、脊椎动物器官和某些哺乳动物味蕾中的表达，旨在阐明ABH相关抗原的生物学意义。研究过程中获得了几个信息，即1）包括人类在内的哺乳动物精母细胞与半乳糖特异性凝集素表现出良好的反应性。抗A抗体和血A特异性凝集素对附睾上皮细胞和精原细胞的染色较强 ...更多信息分别储存在猫和大鼠的附睾中。人雄性生殖器官上皮细胞表达与供体血型相对应的ABH相关抗原，但AOB、H、Se、Le和X基因表达存在组织依赖性差异。（3）尽管ABH抗原仅在鱼类、蛙类、非灵长类哺乳动物和新大陆猴的分泌细胞中表达，但在旧大陆猴的血管内皮细胞和分泌细胞中均有表达。ABH相关抗原在人和类人猿的红细胞、皮脂腺细胞和血管内皮细胞中均有表达，提示ABH相关抗原影响精子的发育和味觉感受器的功能。这些抗原的表达与脊椎动物的进化密切相关，ABO-从新鲜血细胞和石蜡包埋组织中建立了基因型，以执行研究项目。日本猴子和其他哺乳动物的糖基转移酶已经被调查，以扩大这一研究项目。少