ヒト男性生殖器官ABH関連抗原発現メカニズムの組織化学的、分子生物学的解析

ABO血型抗原是本世纪初发现的。虽然目前对ABH及其相关抗原的生物化学和分子生物化学性质已经有了很好的认识，但对这些抗原的生物学意义还没有明确的解释。表征细胞关于其表面和分泌糖缀合物是重要的，以便了解其生理和相互作用行为的基础。本项目研究了ABH相关抗原在哺乳动物乳腺、生殖器官、脊椎动物器官和部分哺乳动物味蕾中的表达，旨在阐明抗原的生物学意义。在研究过程中获得了几个信息，即1)。包括人在内的哺乳动物精母细胞对半乳糖特异性凝集素表现出良好的反应性。抗A抗体和血A特异性香素分别对储存在猫和大鼠的附睾和精子的上皮细胞进行了密集染色。虽然人类男性生殖器官上皮细胞表达与献血者血型相对应的ABH相关抗原，但AOB、H。Se， Le和X基因受组织依赖性差异表达。2)在大鼠、猫、狗、兔、日本猴和人的味蕾细胞中重组了ABH相关抗原。3)虽然ABH抗原仅在鱼、蛙、非灵长类哺乳动物和新大陆猴的分泌细胞中表达，但在旧大陆猴的血管上皮细胞和皮脂细胞中也有表达。抗原在人、人红细胞、皮脂腺细胞和血管上皮细胞中均有表达。这些结果表明，ABH相关抗原影响精子的发育和味觉受体的功能，抗原的表达与脊椎动物的进化密切相关。为实施本研究项目，建立了新鲜血细胞和石蜡包埋组织abo基因型测定方法。为了扩大这一研究项目，研究人员正在日本猴和其他哺乳动物中是否存在编码abo -糖基转移酶的基因。少

The ABO blood group antigens were discovered at the bigining of this century. Althogh the biochemical and molecluar-biochemical nature of the ABH and related antigens is now quite well characterized, no clear explanation of the biological significance of these antigens has been proposed. Characterization of cells with regard to their surface and secretory glycoconjugates is important in order to undestand the basis of their physiological and interactive behavior. In this project the expression of the ABH related antigens in mammalian maile reproductive organs, vertebrate organs and the taste buds of some mammals was investigated for the purpose of elucidating the biological significance of the antigens. During the research several informations were obtained, that is,1).Spermatocytes from mammals including the human showed good reactivity with the lectins specific to galactose. Anti A antibody and blood A specific lentins intensively stained epithelial cells of epididumis and spermatozo … More a stored in the epididymis of cats and rats, respectively. Althogh the epithelial cells from human male reproductive organs expressed ABH related antigens corresponding with the blood groups of the donors, AOB,H.Se, Le and X genes are subjected to a tissue - dependent differential expresion.2) The ABH related antigens were reconganized in the taste buds cells of rats, cats, dogs, rabbits, japanese monkeys and human.3) Althogh the ABH antigens were expressed only in secretory cells of fishes, frogs, non primate mammals and new world monkeys, the antigens were expressed in vascular emdothelial cells and sebretory cells of old world monkeys. The antigens was expressed in red blood cells, sebretory cells and vascular emdothelial cells of anthorpoid and human.These results indicate that the ABH related antigens effect the development of spermatpzoa and the function in the taste receptor, and the expression of the antigens is closely related with the vertebrate evolution.The determination methods of ABO-genetypes from fresh blood cells and parafifn embedded tissue were established in orde to execute the research project.The existence of genes encoding ABO-glycosyltransferase in japanese monkey and other mammals has been invesigating in orde to expand this research project. Less