ヒト男性生殖器官ABH関連抗原発現メカニズムの組織化学的、分子生物学的解析

ABO血型抗原是在本世纪初发现的。尽管ABH及其相关抗原的生物化学和分子生化性质目前已有了很好的描述，但对这些抗原的生物学意义还没有提出明确的解释。为了了解细胞的生理和相互作用行为的基础，细胞表面和分泌糖偶联物的特性是很重要的。本课题研究了ABH相关抗原在哺乳动物的生殖器官、脊椎动物器官和某些哺乳动物的味蕾中的表达，以阐明这些抗原的生物学意义。在研究过程中获得了几个信息，即：1)包括人类在内的哺乳动物精细胞与半乳糖特异性凝集素具有良好的反应性。抗A抗体和血A特异性香菇素对附睾和精索…上皮细胞的强烈染色更多的a分别储存在猫和大鼠的附睾处。虽然人类男性生殖器官上皮细胞表达与供者血型相对应的ABH相关抗原，但AOB、H.Se、Le和X基因受到组织依赖性差异表达。2)ABH相关抗原在大鼠、猫、狗、兔、日本猴和人的味蕾细胞中重组。3)虽然ABH抗原仅在鱼、蛙、非灵长类哺乳动物和新大陆猴的分泌细胞中表达，但这些抗原在旧大陆猴的血管上皮细胞和存储细胞中表达。ABH相关抗原在脊椎动物和人的红细胞、脂肪细胞和血管内皮细胞中均有表达。这些结果表明ABH相关抗原影响精子的发育和味觉感受器的功能，其表达与脊椎动物的进化密切相关。为了实施这一研究项目，建立了新鲜血细胞和石蜡包埋组织中ABO基因的检测方法。为了扩大这一研究项目，已对日本猴子和其他哺乳动物中存在的ABO糖基转移酶基因进行了研究。较少

The ABO blood group antigens were discovered at the bigining of this century. Althogh the biochemical and molecluar-biochemical nature of the ABH and related antigens is now quite well characterized, no clear explanation of the biological significance of these antigens has been proposed. Characterization of cells with regard to their surface and secretory glycoconjugates is important in order to undestand the basis of their physiological and interactive behavior. In this project the expression of the ABH related antigens in mammalian maile reproductive organs, vertebrate organs and the taste buds of some mammals was investigated for the purpose of elucidating the biological significance of the antigens. During the research several informations were obtained, that is,1).Spermatocytes from mammals including the human showed good reactivity with the lectins specific to galactose. Anti A antibody and blood A specific lentins intensively stained epithelial cells of epididumis and spermatozo … More a stored in the epididymis of cats and rats, respectively. Althogh the epithelial cells from human male reproductive organs expressed ABH related antigens corresponding with the blood groups of the donors, AOB,H.Se, Le and X genes are subjected to a tissue - dependent differential expresion.2) The ABH related antigens were reconganized in the taste buds cells of rats, cats, dogs, rabbits, japanese monkeys and human.3) Althogh the ABH antigens were expressed only in secretory cells of fishes, frogs, non primate mammals and new world monkeys, the antigens were expressed in vascular emdothelial cells and sebretory cells of old world monkeys. The antigens was expressed in red blood cells, sebretory cells and vascular emdothelial cells of anthorpoid and human.These results indicate that the ABH related antigens effect the development of spermatpzoa and the function in the taste receptor, and the expression of the antigens is closely related with the vertebrate evolution.The determination methods of ABO-genetypes from fresh blood cells and parafifn embedded tissue were established in orde to execute the research project.The existence of genes encoding ABO-glycosyltransferase in japanese monkey and other mammals has been invesigating in orde to expand this research project. Less