New gene expression vector using duck hepatitis B virus
利用鸭乙型肝炎病毒的新基因表达载体
基本信息
- 批准号:07670564
- 负责人:
- 金额:$ 1.54万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1995
- 资助国家:日本
- 起止时间:1995 至 1996
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
New hepatotropic gene expression vector using the animal model of hepatitis B virus, duck hepatitis B virus (DHBV) was investigated. Plasmid DNA containing wild type of DHBV DNA was complexed with liposome, and injected into the peripheral vein of one-day-old Pekin duck. DHBV DNA was expressed exclusively in the hepatocytes and virus replication was detected. Precore defective mutant was constructed by one base insertion into precore ORF.The mutant DHBV DNA was also expressed and replicated virus was detected in the serum 2 weeks after inoculation, but virus titer in the serum was much less than wild type after 16 weeks. Histological analysis of precore mutant infected liver showed that some of the animals had the inflammation and slight fibrosis which equivalent to the chronic hepatitis, indicating that the pathogenesis related to the precore protein would be suggested and should be analyzed for the next step. The clone with 24 bases insertion in the precore ORF was constructed and inoculated into the ducks resulted the wild type virus replication. The stability and the capacity of foreign gene inserted into DHBV DNA needed to be analyzed.
研究了一种新型亲肝基因表达载体--鸭乙型肝炎病毒(DHBVd)。将含有野生型DHBVDNA的质粒DNA与脂质体复合,注射到1日龄北京鸭的外周静脉中。DHBVDNA仅在肝细胞中表达,并可检测到病毒复制。DHBVDNA突变株在接种后2周即可表达,并在血清中检测到复制病毒,但16周后血清病毒滴度明显低于野生型。前C区突变型感染肝脏的组织学分析显示,部分动物出现炎症和轻度纤维化,相当于慢性肝炎,提示与前C区蛋白相关的发病机制有待下一步分析。构建了在前C区插入24个碱基的克隆,接种鸭体内,获得野生型病毒的复制。外源基因插入DHBVDNA的稳定性和容量有待进一步研究。
项目成果
期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Masami Tagawa: "Gene expression and acrtive virus replication in the liver after injection of duck hepatitis B virus DNA into the peripheral vein of ducklings" Journal of Hepatology. 24. 328-334 (1996)
Masami Takawa:“将鸭乙型肝炎病毒 DNA 注射到小鸭外周静脉后,肝脏中的基因表达和活性病毒复制”《肝脏病学杂志》。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Toshiki Ehata: "Point mutation in precore region of hepatitis B virus : sequential changes from 'wild'to'mutant'" J.Gastroenterol.Hepatol. 11. 566-574 (1996)
Toshiki Ehata:“乙型肝炎病毒前核心区的点突变:从‘野生’到‘突变’的连续变化”J.Gastroenterol.Hepatol。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Masami Tagawa: "Gene expression and acrtive virus replication in the liver after injection of duck hepatitis B virus DNA into the peripheral vein of duckings" Journal of Hepatology. 24. 328-334 (1996)
Masami Takawa:“将鸭乙型肝炎病毒 DNA 注射到鸭子的外周静脉后,肝脏中的基因表达和活性病毒复制”《肝脏病学杂志》。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Tagaw, Masami: "Gene expression and active virus replication in the liver after injection of duck hepatitis B virus DNA into the peripheral vein of ducklings" J.Hepatology. 24. 328-334 (1996)
Tagaw,Masami:“将鸭乙型肝炎病毒 DNA 注射到鸭子的外周静脉后,肝脏中的基因表达和活跃的病毒复制”J.Hepatology。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Ehata, Toshiki: "Point mutation in precore region of hepatitis B virus : sequential changes from 'wild' to 'mutant'" J.Gastroenterol Hepatol. 11. 566-574 (1996)
Ehata,Toshiki:“乙型肝炎病毒前核心区的点突变:从‘野生’到‘突变’的连续变化”J.Gastroenterol Hepatol。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
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TAGAWA Masami其他文献
TAGAWA Masami的其他文献
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{{ truncateString('TAGAWA Masami', 18)}}的其他基金
Evaluation of medical professional identity formation
医疗职业认同形成评价
- 批准号:
18K10008 - 财政年份:2018
- 资助金额:
$ 1.54万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Experiences of medical students and professional identity formation.
医学生的经历和职业认同的形成。
- 批准号:
24590620 - 财政年份:2012
- 资助金额:
$ 1.54万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of objective clinical performance examination based on undergraduate curriculum and psychometric analysis.
基于本科课程和心理测量分析的客观临床表现考试的开发。
- 批准号:
21590569 - 财政年份:2009
- 资助金额:
$ 1.54万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of clinical curriculum with simulated patients and objective assessment method
模拟患者临床课程开发及客观评价方法
- 批准号:
15590449 - 财政年份:2003
- 资助金额:
$ 1.54万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Animal model of chronic hepatitis using duck hapatitis B virus precore mutant infection.
利用鸭乙型肝炎病毒前核突变体感染建立慢性肝炎动物模型。
- 批准号:
09670511 - 财政年份:1997
- 资助金额:
$ 1.54万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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