New gene expression vector using duck hepatitis B virus

点击翻译按钮获取中文摘要

New hepatotropic gene expression vector using the animal model of hepatitis B virus, duck hepatitis B virus (DHBV) was investigated. Plasmid DNA containing wild type of DHBV DNA was complexed with liposome, and injected into the peripheral vein of one-day-old Pekin duck. DHBV DNA was expressed exclusively in the hepatocytes and virus replication was detected. Precore defective mutant was constructed by one base insertion into precore ORF.The mutant DHBV DNA was also expressed and replicated virus was detected in the serum 2 weeks after inoculation, but virus titer in the serum was much less than wild type after 16 weeks. Histological analysis of precore mutant infected liver showed that some of the animals had the inflammation and slight fibrosis which equivalent to the chronic hepatitis, indicating that the pathogenesis related to the precore protein would be suggested and should be analyzed for the next step. The clone with 24 bases insertion in the precore ORF was constructed and inoculated into the ducks resulted the wild type virus replication. The stability and the capacity of foreign gene inserted into DHBV DNA needed to be analyzed.