权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Studies on the pathogenesis of early lesions in Crohn's disease : Clinical and experimental investigation

克罗恩病早期病变发病机制的研究：临床和实验研究

基本信息

批准号：
07670633
负责人：
IIDA Mitsuo
金额：
$ 1.34万
依托单位：
KAWASAKI MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670633/
关键词：
Crohn's disease M cell colonic lymphoid nodule indomethacin small intestinal ulcer electron microscopy proliferative activities of the mucosa growth factor HLA-DR 大腸拡大内視鏡初期病変

项目摘要

1. Clinical investigationTo clarify the role of the follicle associated epithelium (FAE) of colonic lymphoid nodules in the formation of ulcers in Crohn's disease, 10 patients with this disease were studied by magnifying colonoscopy, electron microscopy, and immunohitochemical examination. As a result, scanning electron microscopy of lymphoid nodules surrounded by a red halo without visible erosions by magnifying colonoscopy showed surface erosions 150-120mum in size. Correlation of scanning and transmission electron microscopy revealed residues of FAE including M cells at the edges of the erosions. In immunohistochemical studies, HLA-DR antigen was more strongly expressed in the entire inflamed colonic mucosa in the active stage of Crohn's disease than in the remission stage. Therefore, the red halo appearance surrounding lymphoid follicles seems to preced visible aphthoid ulcers and suggests that ulcerations in Crohn's disease originate from FAE.2. Experimental investigationTo charac … More teriza early changes in both cryptal proliferation and expression of growth factors in indomethacin-induced small intestinal damage, scanning electron microscopic findings, cryptal proliferation (using immunohistochemical staining for Brd U), and intestinal mRNA expression of TGF-beta_1, IGF-1, HGF,and NGF (using RT-PCR) were investigated in rats which were received 24 mg/kg of intracolonic indamethacin. Scanning electron microscopy showed that the villi in the mid-small intestine was distorted with tortuous fashion at an hour. Cryptal proliferation in the mid-small intestine was not altered at 2 hour whereas it was decreased at all sites after 5 hours. IGF-1 and HGF expression became obvious at 6 hour, while NGF could not be detected at this time point. These findings suggest that early mucosal damage induced by indomethacin is characterized by alteration of the crypt architecture without changes in crypt cell proliferation, and that changes of some growth factors may play a role in the progression of the mucosal damage. Less

1.为探讨结肠淋巴结滤泡相关上皮（FAE）在克罗恩病溃疡形成中的作用，对10例克罗恩病患者进行了放大结肠镜、电镜及免疫组化观察。结果，扫描电子显微镜检查淋巴结周围有红色光晕，放大结肠镜检查无可见糜烂，显示表面糜烂大小为150- 120 μ m。扫描和透射电子显微镜的相关性揭示了FAE的残留物，包括侵蚀边缘的M细胞。在免疫组化研究中，HLA-DR抗原在克罗恩病活动期的整个炎症结肠粘膜中的表达比缓解期更强。因此，淋巴滤泡周围的红色晕似乎先于可见的口疮样溃疡，并提示克罗恩病的溃疡起源于FAE。实验研究 ...更多信息本实验观察了吲哚美辛（indamethacin，24 mg/kg）结肠内给药后大鼠小肠粘膜损伤早期隐窝增生和生长因子表达的变化，以及扫描电镜下隐窝增生（BrdU免疫组化染色）和小肠TGF-β 1、IGF-1、HGF和NGF mRNA表达（RT-PCR）的变化。扫描电镜观察发现，1小时后小肠中段绒毛扭曲，呈曲折状。小肠中段的隐窝增殖在2小时时没有改变，而在5小时后在所有部位都有所减少。IGF-1和HGF在6 h时表达明显，而NGF在此时间点未检测到。这些结果表明，吲哚美辛引起的早期粘膜损伤的特点是改变的隐窝结构，而不改变隐窝细胞增殖，和一些生长因子的变化可能在粘膜损伤的进展中发挥作用。少