权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Cellular and Molecular Study of Blood-Nerve Barrier

血神经屏障的细胞和分子研究

基本信息

批准号：
07670703
负责人：
KANDA Takashi
金额：
$ 1.47万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670703/
关键词：
Blood-Nerve Barrier Endothermal Cell Cell Culture Ganglioside Neuropathy Cell Biology ニューロパタ-Endothelial cell blood-nerve barrier ganglioside Antibody neuropathy glycolipid glucose transporten I 血液神経閉門血管内皮細胞細胞接着因子

项目摘要

Penetration of immunoglobulins and/or migration of activated lumphocytes into peripheral nervous system (PNS) parenchyma are the initial key steps to develop immunological disorders of PNS including Guillain-Barre syndrome, IgM neuropathy and chronic inflammatory demyelinating polyradiculoneuropathy. Hence, it is important to know the cellular property of endothelial cells of endoneurial tissue origin (PnMEC) because these cells constitute the bulk of the blood-nerve barrier (BNB). For this purpose we developed a method to isolate and culture pure populations of PnMECs from bovine cauda euina. PnMECs were identified by their cobblestone appearance, immunoreactivity against Factor VIII/von Willebrand factor (vWF) antigen, and positive uptake of Dil-Ac-LDL.The glucose transporter type 1 (GLUT1) expression of these cells was rapidly down-regulated in vitro. Other thatn GM3 (NeuAc) and GM3 (NeuGc) as major glycosphingolipids, PnMECs comprise GM1, GD1a, GD1b and GT1b, which are shared by PNS parenchyma, and sialyl lactosaminyl paragloboside (SLPG) as minor species. Because bovine PnMECs proliferate rapidly and a large mass of cells could be obtained, this method should contribute to the biochemical analysis of surface molecules in PnMECs that might play a key role in the formation of BNB as well as in pathological conditions involving the PNS.

免疫球蛋白的渗透和/或活化的淋巴细胞迁移到周围神经系统（PNS）实质中是PNS免疫性疾病（包括格林-巴利综合征、IgM神经病和慢性炎性脱髓鞘性多神经根神经病）发生的最初关键步骤。因此，了解神经内膜组织来源的内皮细胞（PnMEC）的细胞特性是很重要的，因为这些细胞构成了血神经屏障（BNB）的主体。为此目的，我们开发了一种从牛尾部分离和培养PnMEC纯群体的方法。PnMEC的形态呈鹅卵石样，对凝血因子VIII/血管性血友病因子（vWF）的免疫反应阳性，对Dil-Ac-LDL的摄取呈阳性，其葡萄糖转运蛋白1（GLUT 1）的表达在体外迅速下调。除了GM 3（NeuAc）和GM 3（NeuGc）作为主要鞘糖脂外，PnMEC还包含PNS薄壁组织共有的GM 1、GD 1a、GD 1b和GT 1b，以及作为次要种类的唾液酸乳糖胺基副球糖苷（SLPG）。由于牛PnMEC增殖迅速，可以获得大量的细胞，这种方法应有助于PnMEC的表面分子的生化分析，可能发挥关键作用，在BNB的形成，以及在病理条件下，涉及PNS。