Pathogenesis of HTLV-I positive polymyositis

HTLV-I阳性多发性肌炎的发病机制

• 批准号: 07670721
• 负责人: HIGUCHI Itsuro
• 金额: $ 1.66万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670721/
• 关键词: HTLV-I; Polymyositis; PCR in situ hybridization; HAM; PCR; in situ hybridization; in situ PCR; Polymyositis; ATL

Patients with polymyositis (PM) in Kagoshima, Japan exhibit seropositivity to human T-cell lymphotropic virus type I (HTLV-I), which is higher than in the general population. A direct HTLV-I infection of muscle fibers was previously reported by in situ hybridization (ISH) in a patient dually infected with HIV and HTLV-I.While using the same technique of ISH,we could not detect HTLV-I in patients with PM infected with HTLV-I alone. We obtained biopsied muscle specimens from 5 HTLV-I positive PM patients, 5 HTLV-I negative PM patients, an autopsied muscle specimen from a HAM patient, and a lymph node from a patient with adult T-cell leukemia (ATL) as a positive control. Employing in situ PCR,we found the HTLV-I provirus in some of the CD4-positive infiltrating cells in HTLV-I-positive PM but not within the muscle fibers. Among HTLV-I-positive PM,we performed double staining of immunohistochemistry for CD4 or macrophage and in situ PCR for HTLV-I provirus on the same sections to determine the exact cell types of HTLV-I containing cells. We found HTLV-I in CD4-positive cells but not in macrophages. This finding suggests that most of the HTLV-I containing CD4-positive cells are not macrophages but lymphocytes.

日本鹿儿岛的多发性肌炎(PM)患者表现出对人类T细胞嗜淋巴病毒I型(HTLV-I)的血清阳性，高于普通人群。先前通过原位杂交技术(ISH)在一例HIV和HTLV-I双重感染的患者中报道了肌肉纤维直接HTLV-I感染，而使用相同的ISH技术，我们不能在单独感染HTLV-I的PM患者中检测到HTLV-I。我们采集了5例HTLV-I阳性PM患者、5例HTLV-I阴性PM患者的肌肉活检标本、1例HAM患者的尸检肌肉标本和1例成人T细胞白血病(ATL)患者的淋巴结作为阳性对照。应用原位聚合酶链式反应，我们发现HTLV-I前病毒在HTLV-I阳性PM的部分CD4阳性的浸润性细胞中存在，但在肌纤维内没有。在HTLV-I阳性的PM中，我们在相同的切片上进行了CD4或巨噬细胞的免疫组织化学双重染色和HTLV-I前病毒的原位PCR双重染色，以确定含有HTLV-I的细胞的确切细胞类型。我们在CD4阳性细胞中发现了HTLV-I，但在巨噬细胞中没有发现。这一发现表明，大多数含有HTLV-I的CD4阳性细胞不是巨噬细胞，而是淋巴细胞。

项目成果

I.Higuchi, K.Hashimoto et al.: "In situ polymerase chain reaction detection of HTLV-I provirus and expression of the p53 tumor suppressor gene in infiltrating cells in skeletal muscle from a patient with adult T cell leukemia." Acta Neuropathol. 90. 323-3

I.Higuchi、K.Hashimoto 等人：“原位聚合酶链式反应检测成人 T 细胞白血病患者骨骼肌浸润细胞中的 HTLV-I 原病毒和 p53 肿瘤抑制基因的表达。”

I,Higuchi et al.: "In situ polymerase chain reaction detection of HTLV-I provirusand expression of the p53 tumor suppressor gene in infiltrating cells in skeletal muscle from a patient with adult T cell leukemia." Acta Neuropathologica. 90. 323-327 (1995)

I, Higuchi 等人：“原位聚合酶链反应检测成人 T 细胞白血病患者骨骼肌浸润细胞中的 HTLV-I 原病毒和 p53 肿瘤抑制基因的表达。”

I.Higuchi et al.: "The main HTLV-I harboring cells in the muscles I viral carriers with polymyositis are not macrophages but CD4_+ lymphocyles." Acta Neuropathologica. 92. 358-361 (1996)

I.Higuchi 等人：“患有多发性肌炎的病毒携带者肌肉中主要的 HTLV-I 窝藏细胞不是巨噬细胞，而是 CD4_ 淋巴细胞。”

I.Higuchi et al.: "In situ polymerase chain reaction detection of HTLV-I provirusand expression of the p53 tumor suppressor gene in infiltrating cells in skeletal muscle from a patient with adult T cell leukemia." Acta Neuropathologica. 90. 323-327 (1995)

I.Higuchi 等人：“原位聚合酶链式反应检测成人 T 细胞白血病患者骨骼肌浸润细胞中的 HTLV-I 原病毒和 p53 肿瘤抑制基因的表达。”

I,Higuchi et al.: "The main HTLV-I harboring cells in the muscles I viral carriers with polymyosltis are not macrophages but CD41 Iymphocytes." Acta Neuropathologica. 92. 358-361 (1996)

I, Higuchi 等人：“患有多发性肌炎的病毒携带者肌肉中主要的 HTLV-I 窝藏细胞不是巨噬细胞，而是 CD41 淋巴细胞。”