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Morphological and histological analysis of cardiovascular anomalies in congenital heart disease model rat embryos raised in whole embryo culture

全胚培养先天性心脏病模型大鼠胚胎心血管异常的形态学和组织学分析

基本信息

批准号：
07670857
负责人：
NAKAGAWA Masao
金额：
$ 1.22万
依托单位：
Shiga University of Medical Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

1. Incidence of cardiovascular anomalies in bis-diamine treated rat embryos A bis-diamine administration to pregnant rats could induce cardiovascular anomalies including tetralogy of Fallot, ventral septal defect and patent truncus arteriosus. However, the incidence was different between Sprague-Dawley (SD) and Wister rats. When a single dose of 200 mg bis-diamine was administered to 9.5,10.5 and 11 pregnant day SD and Wister mother rats, the incidences of cardiovascular anomalies were 35.1%, 64.1% and 10.5%, and 100%, 92.8% and 45.6%, respectively.2. Morphological and histological analysis of the heart in Bis-diamin treated and control rat embryos raised in whole embryo culture. Single dose of 200 mg bis-diamine was administered to the pregnant Wister rats on 10.5 pregnant day. The embryos were removed at 6 hours after bis-diamine administration and immediately cultured for 24 and 48 hours following the procedures described by New et al (1973). (1) Morphological analysis : There was n … More o difinite developmental and morphological difference between bis-diamine-treated and control embryos after 24 hours culture. However, poor development of the truncal ridges could be observed in bis-diamine treated embtryos after 48 hours culture, when compared with control embyos. (2) Histological analysis : In the poorly developed truncal ridges of the bis-diamine treated embryos after 48 hours culture, there were less HNK-1 or N-CAM immunoreactive mesenchymal cells in comparison with control embryos. Furthermore, a HNK-1 or N-CAM immunoreactive chain from the neural crest to the truncus arteriosus via the 3rd, 4th and 6th aortic arches, which was clearly recognized in the control embryos after 24 or 48 hours culture, could not be detected in the bis-diamine treated ones. These findings suggested that bis-diamine prevented neural crest cells from normal migration into the heart or transformation into truncal mesenchymal cells, inducing abnormal truncal division and subsequent cardiac defects with truncal anomalies. Less

1. 妊娠大鼠经双二胺处理后，可引起法洛四联症、腹间隔缺损、动脉干未闭等心血管异常。然而，Sprague-Dawley （SD）和Wister大鼠的发生率不同。妊娠期SD大鼠和Wister大鼠分别为9.5、10.5和11只，单次给药200 mg时，心血管异常发生率分别为35.1%、64.1%和10.5%,100%、92.8%和45.6%。双二胺处理和对照大鼠全胚培养心脏形态学和组织学分析。Wister大鼠妊娠10.5天单次给药200 mg双二胺。在双二胺给药后6小时取出胚胎，并按照New等人（1973）描述的程序立即培养24和48小时。(1)形态学分析：培养24 h后，双二胺处理的胚胎与对照的发育和形态学差异不明显。然而，与对照胚胎相比，经双二胺处理的胚胎在培养48小时后，其躯干嵴发育较差。(2)组织学分析：培养48h后，经双二胺处理的胚胎，在发育不良的干脊中，HNK-1或N-CAM免疫反应性间充质细胞较少。此外，从神经嵴经第3、第4和第6主动脉弓到动脉干的HNK-1或N-CAM免疫反应链在培养24或48小时后的对照胚胎中被清楚地识别出来，而在双二胺处理的胚胎中则没有检测到。这些结果表明，双二胺阻止神经嵴细胞向心脏的正常迁移或向截骨间充质细胞的转化，导致截骨分裂异常，导致心脏缺损伴截骨异常。少