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Morphological and histological analysis of cardiovascular anomalies in congenital heart disease model rat embryos raised in whole embryo culture

全胚培养先天性心脏病模型大鼠胚胎心血管异常的形态学和组织学分析

基本信息

批准号：
07670857
负责人：
NAKAGAWA Masao
金额：
$ 1.22万
依托单位：
Shiga University of Medical Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

1. Incidence of cardiovascular anomalies in bis-diamine treated rat embryos A bis-diamine administration to pregnant rats could induce cardiovascular anomalies including tetralogy of Fallot, ventral septal defect and patent truncus arteriosus. However, the incidence was different between Sprague-Dawley (SD) and Wister rats. When a single dose of 200 mg bis-diamine was administered to 9.5,10.5 and 11 pregnant day SD and Wister mother rats, the incidences of cardiovascular anomalies were 35.1%, 64.1% and 10.5%, and 100%, 92.8% and 45.6%, respectively.2. Morphological and histological analysis of the heart in Bis-diamin treated and control rat embryos raised in whole embryo culture. Single dose of 200 mg bis-diamine was administered to the pregnant Wister rats on 10.5 pregnant day. The embryos were removed at 6 hours after bis-diamine administration and immediately cultured for 24 and 48 hours following the procedures described by New et al (1973). (1) Morphological analysis : There was n … More o difinite developmental and morphological difference between bis-diamine-treated and control embryos after 24 hours culture. However, poor development of the truncal ridges could be observed in bis-diamine treated embtryos after 48 hours culture, when compared with control embyos. (2) Histological analysis : In the poorly developed truncal ridges of the bis-diamine treated embryos after 48 hours culture, there were less HNK-1 or N-CAM immunoreactive mesenchymal cells in comparison with control embryos. Furthermore, a HNK-1 or N-CAM immunoreactive chain from the neural crest to the truncus arteriosus via the 3rd, 4th and 6th aortic arches, which was clearly recognized in the control embryos after 24 or 48 hours culture, could not be detected in the bis-diamine treated ones. These findings suggested that bis-diamine prevented neural crest cells from normal migration into the heart or transformation into truncal mesenchymal cells, inducing abnormal truncal division and subsequent cardiac defects with truncal anomalies. Less

1.双二胺处理的大鼠胚胎中心血管畸形的发生率对妊娠大鼠施用双二胺可诱导心血管畸形，包括法洛四联症、腹侧间隔缺损和动脉干未闭。但SD大鼠和Wister大鼠的发病率不同。孕9.5、10.5和11天的SD和Wister母鼠单次注射200 mg双二胺，心血管畸形发生率分别为35.1%、64.1%和10.5%，100%、92.8%和45.6%.在全胚胎培养中培养的双二胺处理和对照大鼠胚胎的心脏形态学和组织学分析。在妊娠第10.5天对妊娠Wister大鼠单次给予200 mg双二胺。在给予双二胺后6小时取出胚胎，并按照New等人（1973）描述的程序立即培养24和48小时。(1)形态学分析：有n ...更多信息 o培养24小时后，双二胺处理的胚胎和对照胚胎之间存在明确的发育和形态学差异。然而，与对照胚胎相比，双二胺处理的胚胎在培养48小时后，可以观察到干脊的发育较差。(2)组织学分析：培养48小时后，在双二胺处理的胚胎的发育不良的干嵴中，与对照胚胎相比，HNK-1或N-CAM免疫反应性间充质细胞较少。此外，HNK-1或N-CAM免疫反应链从神经嵴动脉干通过第3，第4和第6主动脉弓，这是清楚地认识到在对照胚胎培养24或48小时后，不能检测到在双二胺处理的。这些结果表明，双二胺阻止神经嵴细胞正常迁移到心脏或转化为躯干间充质细胞，诱导异常的躯干分裂和随后的心脏缺陷与躯干异常。少