Developmental defects of coronary vasculature in rat embryos administered bis-diamine and WKY/NCrj rat embryos

给予双二胺的大鼠胚胎和 WKY/NCrj 大鼠胚胎中冠状脉管系统的发育缺陷

基本信息

  • 批准号:
    11670756
  • 负责人:
  • 金额:
    $ 1.79万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1999
  • 资助国家:
    日本
  • 起止时间:
    1999 至 2000
  • 项目状态:
    已结题

项目摘要

1. A single 200-mg dose of bis-diamine was administered to pregnant Wister rats at 10.5 days of gestation. Fifty-two embryos from 10 mother rats underwent morphological analysis of the coronary arteries. Conotruncal anomalies were detected in 48 of 52 embryos, and an aplastic or hypoplastic left coronary artery was found in all of them.2. Coronary vasculature was first detected in the heart of at 14.5 embryonic day (ED) control rat embryo. However, in the herats of bis-diamine treated embryos, poor promotion of epicardial-endothelial transformation was revealed at ED 14.5, 15.5,16.5, and 17.5, causing abnormal development of the coronary vasculature. V-CAM and tenascine were less expressed in the coronary vascular endothelial cells. Sinusoids were widely and deeply extended in the thin ventricular wall.3. Developmental analysis of the early bis-diamine treated rat embryos using whole embryo culture, disclosed a markedly elongated outflow tract and enlarged ventricle with thin myocardial wall before the truncal division. Histological examinations detected no coronary buds and sparse N-CAM positive cells around the arterial trunk. This was also the case with the early WKY/NCrj rat embryos.4. When bis-diamine was administered to the 10, 11, and 12 pregnant day mother rats, abnormal conotruncal division was seen in 0%, 93%, and 32% of the embryos, respectively. Furthermore, anomalous coronary arteries were detected only when bis-diamine was administered on 11 and 12 pregnant day, and the incidence was coincident with that of anomalous truncal division.Although not being able to clarify whether neural crest cells play a role in coronary bud formation, these studies suggested that abnormal truncal division and pericardial development caused anomalous coronary vascular formation and distribution.
1.在妊娠10.5天时,对妊娠Wister大鼠单次给予200 mg双二胺。对10只母鼠的52只胚胎进行冠状动脉形态学分析。结果:1. 52例胚胎中有48例出现圆锥动脉干异常,均为左冠状动脉发育不全或发育不良.首次在胚胎14.5天(艾德)对照大鼠胚胎的心脏中检测到冠状血管。然而,在双二胺处理的胚胎中,在艾德14.5、15.5、16.5和17.5的ED下,显示出对心外膜-内皮转化的不良促进,导致冠状血管的异常发育。V-CAM和tenascine在冠状动脉内皮细胞中表达较少。窦状隙在较薄的心室壁内广泛而深入.使用全胚胎培养物对早期双二胺处理的大鼠胚胎进行发育分析,发现在干分裂前流出道明显延长,心室扩大,心肌壁变薄。组织学检查未发现冠状动脉芽,动脉主干周围有稀疏的N-CAM阳性细胞。早期WKY/NCrj大鼠胚胎的情况也是如此。4.当双二胺给药于妊娠10、11和12天的母鼠时,分别在0%、93%和32%的胚胎中观察到异常圆锥干分裂。此外,只有在妊娠第11和12天给予双二胺时才发现异常冠状动脉,其发生率与异常干细胞分裂的发生率相一致,虽然不能明确神经嵴细胞是否在冠状动脉芽形成中起作用,但这些研究表明,异常干细胞分裂和心包发育导致异常冠状动脉血管的形成和分布。

项目成果

期刊论文数量(18)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nakagawa M: "Teratogenic effect of bis-diamine on embryonic rat heart"Cong Anom. 40. 157-161 (2000)
Nakakawa M:“双二胺对胚胎大鼠心脏的致畸作用”Cong Anom。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Nakagawa M: "Ebstein's anomaly associated with trisomy 9p."Clin Genet. 55. 383-385 (1999)
Nakakawa M:“Ebstein 异常与 9p 三体有关。”Clin Genet。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Setsuko Nishijima: "Teratogenic effect of bis-diamine on early embryonic rat heart : an in vitro study."Teratology. 62・(2). 115-122 (2000)
Setsuko Nishijima:“双二胺对早期胚胎大鼠心脏的致畸作用:体外研究。”畸胎学 62・(2) 115-122 (2000)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Nakagawa M: "Tetracuspid aortic valve associated with a 22q11.2 microdeletion"Am J Med Genet. 93. 74-75 (2000)
Nakakawa M:“与 22q11.2 微缺失相关的四尖瓣主动脉瓣”Am J Med Genet。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Masao Nakagawa: "Etiology and Morphogenesis of Congenital Heart Disease, Clark EB, Markwald RR, Takao A, Nakazawa M eds."Futura Publishing Co (分担執筆). 389 (2000)
Masao Nakakawa:“先天性心脏病的病因学和形态发生,Clark EB、Markwald RR、Takao A、Nakazawa M eds。”Futura Publishing Co(撰稿人)389(2000)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

NAKAGAWA Masao其他文献

NAKAGAWA Masao的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('NAKAGAWA Masao', 18)}}的其他基金

Identification of molecular basis of acute-and lymphoma-type adult T-cell leukemia/lymphoma
急性和淋巴瘤型成人 T 细胞白血病/淋巴瘤的分子基础鉴定
  • 批准号:
    21790930
  • 财政年份:
    2009
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Developmental analyses of the cardiac conduction system and cardiomyocytic functions in the rats with congenital cardiac anomalies
先天性心脏异常大鼠心脏传导系统及心肌细胞功能的发育分析
  • 批准号:
    21591386
  • 财政年份:
    2009
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Developmental analysis of the proximal portion and orifices of the coronary arteries and the role of extracardiac cells in their morphogenesis
冠状动脉近端部分和孔口的发育分析以及心外细胞在其形态发生中的作用
  • 批准号:
    19591203
  • 财政年份:
    2007
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Array CGH analytics and gene expression analytics revealed distinct subgroups in Peripheral T cell lymphomas
阵列 CGH 分析和基因表达分析揭示了外周 T 细胞淋巴瘤的不同亚组
  • 批准号:
    19790678
  • 财政年份:
    2007
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Research on Visible Light Communication
可见光通信研究
  • 批准号:
    17206041
  • 财政年份:
    2005
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Analysis of coronary vascular development in an animal model of congenital heart disease - in vivo and in vitro studies to detect a role of proepicardial organ derived cells
先天性心脏病动物模型中冠状血管发育的分析 - 体内和体外研究以检测心外膜器官衍生细胞的作用
  • 批准号:
    16390299
  • 财政年份:
    2004
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Teratologic effects of Bis-diamine on cardiovascular development in rat embryo : in vivo and in vitro analysis
双二胺对大鼠胚胎心血管发育的致畸作用:体内和体外分析
  • 批准号:
    09670800
  • 财政年份:
    1997
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Morphological and histological analysis of cardiovascular anomalies in congenital heart disease model rat embryos raised in whole embryo culture
全胚培养先天性心脏病模型大鼠胚胎心血管异常的形态学和组织学分析
  • 批准号:
    07670857
  • 财政年份:
    1995
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The featuresof rats with hypertrophic cardiomyopathy and rats with hypertension.
肥厚型心肌病大鼠和高血压大鼠的特点。
  • 批准号:
    06670737
  • 财政年份:
    1994
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Morphological analysis of cardiac malformations in developing WKY/NCrj rat embryos raised in whole embryo
全胚培养WKY/NCrj大鼠胚胎心脏畸形的形态学分析
  • 批准号:
    05670671
  • 财政年份:
    1993
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

相似海外基金

Living with Spontaneous Coronary Artery Dissection: A multicentre, patient-informed investigation
自发性冠状动脉夹层的生活:一项多中心、患者知情的调查
  • 批准号:
    481005
  • 财政年份:
    2023
  • 资助金额:
    $ 1.79万
  • 项目类别:
Development of nextgeneration cellular artificial blood vessels for coronary artery bypass surgery using bio-3D printer
使用生物 3D 打印机开发用于冠状动脉搭桥手术的下一代细胞人造血管
  • 批准号:
    23H02991
  • 财政年份:
    2023
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Whole genome sequence interpretation for lipids to discover new genes and mechanisms for coronary artery disease
脂质的全基因组序列解释,以发现冠状动脉疾病的新基因和机制
  • 批准号:
    10722515
  • 财政年份:
    2023
  • 资助金额:
    $ 1.79万
  • 项目类别:
Molecular predictors of cardiovascular events and resilience in chronic coronary artery disease
心血管事件的分子预测因素和慢性冠状动脉疾病的恢复力
  • 批准号:
    10736587
  • 财政年份:
    2023
  • 资助金额:
    $ 1.79万
  • 项目类别:
CORONARY ARTERY RISK DEVELOPMENT IN YOUNG ADULTS (CARDIA) STUDY - UNIVERSITY OF MINNESOTA FIELD CENTER.
年轻人冠状动脉风险发展 (CARDIA) 研究 - 明尼苏达大学实地中心。
  • 批准号:
    10901060
  • 财政年份:
    2023
  • 资助金额:
    $ 1.79万
  • 项目类别:
CORONARY ARTERY RISK DEVELOPMENT IN YOUNG ADULTS (CARDIA) STUDY - COORDINATING CENTER (CC)
年轻人冠状动脉风险发展 (CARDIA) 研究 - 协调中心 (CC)
  • 批准号:
    10901063
  • 财政年份:
    2023
  • 资助金额:
    $ 1.79万
  • 项目类别:
Repetitive Stretch-Induced Myocardial Stiffening in Chronic Coronary Artery Disease
慢性冠状动脉疾病中反复牵拉引起的心肌硬化
  • 批准号:
    10588929
  • 财政年份:
    2023
  • 资助金额:
    $ 1.79万
  • 项目类别:
Sex-differences in 5 year survival with percutaneous coronary intervention compared to coronary artery bypass graft surgery in patients with diabetes and multivessel disease
糖尿病和多支血管疾病患者经皮冠状动脉介入治疗与冠状动脉搭桥手术的 5 年生存率存在性别差异
  • 批准号:
    495441
  • 财政年份:
    2023
  • 资助金额:
    $ 1.79万
  • 项目类别:
DCAD - Developing better diagnostics for Coronary Artery Disease with novel AI-enhanced, ultra fast proteomics
DCAD - 利用新型 AI 增强型超快速蛋白质组学开发更好的冠状动脉疾病诊断方法
  • 批准号:
    10072712
  • 财政年份:
    2023
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Collaborative R&D
ADAR mediated RNA editing is a causal mechanism in coronary artery disease
ADAR 介导的 RNA 编辑是冠状动脉疾病的因果机制
  • 批准号:
    10629687
  • 财政年份:
    2023
  • 资助金额:
    $ 1.79万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了