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Investigation of the Relationship between Invasion of Fungi and Alteration of Cell Kinetics in Hair Apparatus in Experimental Dermatophytosis, Especially in Comparison with Human Hair Fungal Infection

实验性皮肤癣菌病中真菌侵袭与毛发装置细胞动力学改变之间关系的研究，特别是与人类毛发真菌感染的比较

基本信息

批准号：
07670933
负责人：
ITO Masaaki
金额：
$ 1.28万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

It is well known that the experimental Trichophyton (T) dermatophytosis produced in hairy animals shows natural healing within a few weeks, but human dermatophytosis caused by T is usually apt to be uncurable without therapy. In the present study, in order to elucidate the process of the natural healing of the experimental dermatophytosis, a strain of T mentagrophytes was inoculated in the guinea pig back skin by a wet disc method and the skin lesions were successively biopsied and examined. Histologically, inflammatory changes in the epidermis and upper dermis reached the maximal state on day 14. Most of the hair apparatus began to retract their hair bulbs on day 16. It was shown by periodic acid-Schiff staining that the infected fungi intruded down into the keratinizing zone of the hair cortex, although few inflammatory cells infiltrated around the lower part of the hair apparatus. By BrdU staining, BrdU-labeling indices (LI) in the hair bulbs as well as in the basal layrs of the epi … More dermis and follicular epithelia significantly increased on day 14. Subsequently, the BrdU-LI became lower and lower with retraction of hair bulbs. From these observations, although hyperproliferation of the epidermis is a factor for the natural healing of the experimental dermatophytosis, it is strongly suggested that fungal infection may first evoke proliferative changes in the hair bulbs as well as in the epidermis, and, then, induce retraction of the hair bulbs and falling away of hairs, resulting in complete elimination of the infectinf fungi. The infecting fungi appear to directly affect the cell kinetics in hair bulbs. On the other hand, in human dermatophytosis caused by various kinds of T,especially when the hair apparatus is affected, the fungi invade and intrude into the keratinizing zone of the hair cortex ; however, the hair apparatus seldom shows retraction of the hair bulb, although the produced hair becomes atrophic (Arch Dermatol Res 283 : 233,1991). If it is assuming that the ability of tissue injury by T is the same both in animal and human skin tissues, the contradictious findings of animal and human hair apparatus infected by T may be due to structural differences, such as difference in size of hair bulb, differences in cell kinetics in hair bulbs, including hair cycle, and/or difference in tolerance of hair bulbar cells to the poison from the fungi, between animals and human beeings. Less

众所周知，毛状动物产生的毛癣(T)实验性皮肤癣在几周内自然愈合，但由T引起的人类皮肤癣通常不经治疗无法治愈。在本研究中，为了阐明实验性皮肤真菌的自然愈合过程，采用湿盘法在豚鼠背部皮肤上接种了一株植T菌，并对皮肤病变进行了活检和检查。组织学上，表皮和真皮上部的炎症变化在第14天达到最大状态。在第16天，大部分毛发开始缩回毛球。周期性的酸希夫染色显示，感染的真菌向下侵入了头发皮层的角化区，尽管很少有炎症细胞浸润在毛发器官的下部。BrdU染色结果显示，第14天毛球和外皮基底层的BrdU标记指数（LI）显著增加，真皮和毛囊上皮明显增多。随后，BrdU-LI随着毛球的缩回而越来越低。从这些观察结果来看，虽然表皮的过度增生是实验性皮肤真菌病自然愈合的一个因素，但强烈建议真菌感染可能首先引起毛球和表皮的增殖变化，然后诱导毛球缩回和头发脱落，导致感染真菌的完全消除。感染真菌似乎直接影响毛球的细胞动力学。另一方面，在由各种T引起的人类皮肤真菌病中，特别是当毛发器官受到影响时，真菌侵入并侵入到毛发皮层的角化区；然而，毛发装置很少显示出毛球的收缩，尽管产生的头发变得萎缩（Arch Dermatol Res 283: 233,1991）。如果假设T对动物和人类皮肤组织的组织损伤能力相同，那么动物和人类毛发器官感染T的矛盾发现可能是由于动物和人类之间的结构差异，如毛球大小的差异，毛球细胞动力学的差异，包括毛发周期，和/或毛球细胞对真菌毒性的耐受性差异。少