An improvement of insect cell-free protein synthesis system

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In the present study, we analyzed an enhancer sequence composed of Malacosma Neustria nucleopolyhedrovirus polyhedorin 5'-UTR for improvement of insect cell-free protein synthesis system. As a result, we found important sequence of 16 base length. A protein synthesis activity using twice repeat of the sequence as enhancer was 1.4-fold greater than that of using usual enhancer. Furthermore, we constructed cell-free expression and analysis systems for organic solvent resistant tyrosinase(metal protein) and thermostable laccase(membrane-binding protein)