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Effect of man-made and asbestos fibers on Human Umbilical Vein Endothelial Cell

人造纤维和石棉纤维对人脐静脉内皮细胞的影响

基本信息

批准号：
07807046
负责人：
KARASAKI Yuji
金额：
$ 1.41万
依托单位：
University of Occupational and Environmental Health
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07807046/
关键词：
Asbestos Thrombomodulin Man Made Mineral Fiber Procoagulant Activity Human Umbilical Vein Endothelial Cell DNA合成タンパク質合成

项目摘要

The potential influences of crocidolite asbestos fibers and man made mineral fibers (potassium titanate whisker and magnesium sulfate whisker) on a procoagulant system of human umbilical vein endothelial cells (HUVECs) were investigated by measuring the activity and antigen level of thrombomodulin (TM) on the cell surface. Statistically significant increases in both the TM activity and TM antigen level were observed on HUVECs treated with crocidolite asbestos fibers for 48h and 72h compared to untreated cells at low concentrations of the fibers which showed no sign of a cytotoxic effect on the cells. An extensive increase in both the TM activity and TM antigen level was also observed on HUVECs treated with potassium titanate whisker or magnesium sulfate whisker for 48h and 72h. A statistical analysis revealed that these fibers had almost the same effects on the increases in both TM activity and the TM antigen level of HUVECs treated with the fibers for 48 h and 72 h, but a treatment of magnesium sulfate whisker at more than 1.25 mu g/ml for 24h was slightly more effective in increasing TM activity on HUVECs compared to other fibers (p<0.05). The [3H] leucine incorporation in HUVECs increased when the cells were treated with crocidolite asbestos or man-made mineral fibers (MMMFs), indicating that the increases in TM activity and the TM antigen level on HUVECs directly exposed to those fibers may not reflect the sole induction of anticoagulant activities, but the general cell damage induced by the fibers.

通过测定人脐静脉内皮细胞（HUVECs）表面血栓调节蛋白（TM）的活性和抗原水平，研究了青石棉纤维和人造矿物纤维（钛酸钾晶须和硫酸镁晶须）对HUVECs促凝系统的潜在影响。与低浓度纤维的未处理细胞相比，在用青石棉纤维处理48小时和72小时的HUVEC上观察到TM活性和TM抗原水平的统计学显著增加，所述纤维未显示对细胞的细胞毒性作用的迹象。用钛酸钾晶须或硫酸镁晶须处理HUVECs 48 h和72 h后，TM活性和TM抗原水平均显著升高。统计学分析显示，这些纤维对用纤维处理48小时和72小时的HUVEC的TM活性和TM抗原水平的增加具有几乎相同的效果，但是与其他纤维相比，以大于1.25 μ g/ml的硫酸镁晶须处理24小时在增加HUVEC的TM活性方面稍微更有效（p<0.05）。当用青石棉或人造矿物纤维（MMMF）处理HUVECs时，[3 H]亮氨酸掺入增加，表明直接暴露于这些纤维的HUVECs上TM活性和TM抗原水平的增加可能不反映单独的抗凝活性诱导，而是由纤维诱导的一般细胞损伤。