Study on the model for the vulnerability to stress using stress-induced c-fos mRNA expression as an index of stress response.

以应激诱导的c-fos mRNA表达作为应激反应指标的应激脆弱性模型研究。

• 批准号: 07807092
• 负责人: WATANABE Yoshifumi
• 金额: $ 1.34万
• 依托单位: SAITAMA MEDICAL SHCOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07807092/
• 关键词: vulnerability to stress; c-fos mRNA; in situ hybridization; Wistar Kyoto rats; paraventricle nucleus; locus coeruleus; Corticosterone; c-fos; In Situハイブリダイゼーション; 血中コルチコステロン濃度; ストレス; 脆弱性; モデル動物; in situ ハイブリダイゼーション

1.Strain comparison in c-fos mRNA expression induced by acute and repeated restraint stress.Wistar Kyoto (WKY) rats and Wistar rats were stressed by 1h restraint stress daily for one, three, seven and fourteen days. In these rats, stress-induced c-fos mRNA expression in paraventricle nucleus (PVN) and locus coeruleus (LC) were measured by in situ hybridization assay.To an acute restraint stress, WKY rats and Wistar rats showed similar c-fos mRNA expression in both PVN and LC.As reported before, Wistar rats showed substantial decrease in stress-induced c-fos mRNA expression in both PVN and LC on third stress day, and thereafter stress-induced c-fos mRNA expression decreased gradually. WKY rats, also, showed similar pattern of decrease in stress-induced c-fos mRNA expression in both PVN and LC by repeated restraint stress. Administration of imipramine, an antidepressant, for two weeks with repeated 1h restraint stress did not affect the decrement of stress-induced c-fos mRNA expression i … More n both PVN and LC in WKY rats.These results suggest that WKY rats are not sensitive and vulnerable to stress.2.Strain comparison in stress response of HPA axis and stress-induced anxiety.Plasma corticosterone (CS) were measured during 2h restraint stress on first, third, sixth and thirteenth stress day in WKY rats and Wistar rats.To an acute restraint stres, WKY rats showed higher peak CS level compared to Wistar rats. Wistar rats showed decrease in peak CS levels and other CS levels at 30min, 60min and 120min after the beginning of the stress from third stress day, and thereafter the decrement of CS levels were getting greater by thirteenth stress day, whereas WKY rats did not show the decrease in peak CS level by thirteenth stress day, and only CS levels at 120min after the beginning of the stress decreased from third stress day.To measure the stress-induced anxiety, we measures the freezing time after fifteen minits footshock stress. Wistar rats showed reduction of the freezing time from fifth stress day, whereas WKY rats did not show any reduction of the freezing time by thirteenth stress day.These results suggest the sensitivity and the vulnerability of WKY rats to stress. Less

1.急性和重复束缚应激诱导c-fos mRNA表达的品系比较Wistar京都（WKY）大鼠和Wistar大鼠每天进行1h束缚应激，持续1、3、7和14 d。在急性束缚应激下，WKY大鼠和Wistar大鼠室旁核（PVN）和蓝斑（LC）c-fos mRNA表达水平相似，Wistar大鼠在应激第3天PVN和LC c-fos mRNA表达水平均显著降低，此后，应激诱导的c-fos mRNA表达逐渐降低。WKY大鼠，也表现出类似的模式，减少应激诱导的c-fos mRNA表达的PVN和LC的重复约束应激。给予抗抑郁药丙咪嗪2周并重复1h束缚应激，不影响应激诱导的c-fos mRNA表达的减少， ...更多信息 2.大鼠HPA轴应激反应和应激焦虑的品系比较分别于应激第1、3、6、13天对WKY大鼠和Wistar大鼠进行束缚应激2 h后血浆皮质酮（corticosterone，CS）的测定，发现WKY大鼠在急性束缚应激时，血浆皮质酮峰值明显高于Wistar大鼠。Wistar大鼠从应激第3天开始，应激后30、60、120分钟CS峰值及其他CS水平均下降，此后至应激第13天下降幅度更大，而WKY大鼠至应激第13天CS峰值未下降，应激开始后120 min时CS水平从第3天开始下降。为了测量应激引起的焦虑，我们测量了15分钟足底电击应激后的冻结时间。Wistar大鼠从应激第5天开始出现冻结时间缩短，而WKY大鼠到应激第13天冻结时间没有减少，提示WKY大鼠对应激的敏感性和脆弱性。少