Analysis of gene expression after chronic restraint stress in the animal model of depression

抑郁动物模型慢性束缚应激后基因表达分析

• 批准号: 14570926
• 负责人: WATANABE Yoshifumi
• 金额: $ 2.24万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570926/
• 关键词: stress; vulnerability; Fisher 344; hypothalamus; gene expression; うつ病動物モデル; 遺伝子探索; ストレス脆弱性; 視床下部; Fisher344ラット

Stress is known to be involved in the development of psychopathologies such as depression. In the present study, we searched for differentially expressed genes in the two species of rats (Fisher 344 and Sprague-Dawley(SD)) after chronic restraint stress. Experiments were conducted by comparing gene expressions in three discrete brain areas (hypothalamus, hippocampus, and cerebral cortex) of male Fisher 344 rats, a valid stress-vulnerable model in comparison with those in male SD rats. We used the microarray system for screening differentially expressed genes in hypothalamus of the animals. Using PCR to confirm the mRNA amounts, we identified two genes whose expression levels differed between Fisher 344 and SD rats. In hypothalamus, transcript levels of cytochrome c oxidase (COX)(subunit IV) of Fisher 344 rats were lower than those of SD. In contrast, the levels of COX were higher in hippocampus of Fisher 344 rats than those of SD rats. The expressions of phosphorylase b kings e in the three brain areas of Fisher 344 rats were higher than those of SD rats. The alterations in expression of these two genes, which are related to processes of energy metabolism, may cause impairment of neuronal function after chronic stress and be related to the development of stress associated psychopathological disorders

众所周知，压力与抑郁症等精神病理学的发展有关。在本研究中，我们寻找差异表达的基因在两个物种的大鼠（费舍尔344和SD）后，慢性束缚应激。通过比较雄性Fisher 344大鼠（一种有效的应激易感模型）与雄性SD大鼠的三个离散脑区（下丘脑、海马和大脑皮质）的基因表达来进行实验。我们利用基因芯片系统筛选动物下丘脑差异表达基因。使用PCR来确认mRNA量，我们确定了两个基因的表达水平在Fisher 344和SD大鼠之间存在差异。下丘脑细胞色素c氧化酶（考克斯）亚单位IV的转录水平在Fisher344大鼠低于SD大鼠。而Fisher 344大鼠海马考克斯水平高于SD大鼠。磷酸化酶B kings e在Fisher 344大鼠三个脑区的表达均高于SD大鼠。这两个基因与能量代谢过程有关，其表达的改变可能导致慢性应激后神经元功能的损害，并与应激相关的精神病理学障碍的发展有关