Cllnlcals study on the concentration by using a new ion chromatography of lonized trace elements and electrolytes in body flulds of patlents

新型离子色谱法测定胃肠道疾病患者体液中游离微量元素和电解质浓度的临床研究。建立了离子色谱法同时测定体液（血清、胆汁、胰液和尿液）中离子化微量元素（Fe^<2+>、Zn^<2+>、Mn^<2+>）、电解质（Ca^<2+>、Mg^<2+>）和NH_<4+>的新方法。在胃肠道疾病患者中，肝内胆管结石患者血清Mg^<2+>浓度明显升高（p<0.05）。肝功能障碍患者血清锌总浓度低于正常人，但血清Zn^<2+>浓度高于正常人（p<0.05）。因此，锌的电离率明显升高。Zn^<2+>浓度与血清白蛋白水平（r=0.62）或血清白蛋白前水平（r=0.73）呈负相关。胆红素钙结石患者胆汁中Ca^<2+>浓度显著升高。Mg^<2+>浓度略有升高。证实Ca^<2+>和Mg^<2+>与总胆汁酸存在相关性。特别是Mg^<2+>与鹅去氧胆酸有相关性。推测胆汁酸可能通过与Mg^<2+>结合进入胆汁排出体外。总之，离子色谱技术可用于体液离子水平的分析，可用于肝功能的评价和胃肠道疾病的诊断。

Clinicals study on the concentration by using a new ion chromatography of ionized trace elements and electrolytes in body fluids of patients with gastrointestinal disease.A new technique of ionchromatography was established for the simultaneous measurement of ionized trace elements (Fe^<2+>, Zn^<2+>, Mn^<2+>), electrolytes (Ca^<2+>, Mg^<2+>) and NH_<4+> in body fluids (serum, bile, pancreatic juice and urine). Among patients with gastrointestinal disease, Mg^<2+> concentration in serum was noticed to increase significantly in patients with hepatolithiasis (p<0.05).Although total concentration of zinc in serum of patients with liver dysfunction was lower than in normal subjects, serum Zn^<2+> concentration was higher (p<0.05). Therefore, the ionized ratio of zinc was found to elevate significantly. There was a negative correlationship between the concentration of Zn^<2+> and serum albumin level (r=0.62) or serum prealbumin level (r=0.73). The concentration of Ca^<2+> elevated significantly in bile of patients with calcium bilirubinate stone. The concentration of Mg^<2+> slightly elevated. It was confirmed that Ca^<2+> and Mg^<2+> showed a correlationship with total bile acid. In particular, Mg^<2+> was found to have a correlation with chenodeoxycholic acid. It was suggested that the bile acid might be excreted by binding with Mg^<2+> into the bile.In conclusion, a new technique of ion chromatography may be useful for analysis of humoral ion levels whith can be applied to evaluate liver functions and diagnose as gastrointestinal diseases.