Cllnlcals study on the concentration by using a new ion chromatography of lonized trace elements and electrolytes in body flulds of patlents

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Clinicals study on the concentration by using a new ion chromatography of ionized trace elements and electrolytes in body fluids of patients with gastrointestinal disease.A new technique of ionchromatography was established for the simultaneous measurement of ionized trace elements (Fe^<2+>, Zn^<2+>, Mn^<2+>), electrolytes (Ca^<2+>, Mg^<2+>) and NH_<4+> in body fluids (serum, bile, pancreatic juice and urine). Among patients with gastrointestinal disease, Mg^<2+> concentration in serum was noticed to increase significantly in patients with hepatolithiasis (p<0.05).Although total concentration of zinc in serum of patients with liver dysfunction was lower than in normal subjects, serum Zn^<2+> concentration was higher (p<0.05). Therefore, the ionized ratio of zinc was found to elevate significantly. There was a negative correlationship between the concentration of Zn^<2+> and serum albumin level (r=0.62) or serum prealbumin level (r=0.73). The concentration of Ca^<2+> elevated significantly in bile of patients with calcium bilirubinate stone. The concentration of Mg^<2+> slightly elevated. It was confirmed that Ca^<2+> and Mg^<2+> showed a correlationship with total bile acid. In particular, Mg^<2+> was found to have a correlation with chenodeoxycholic acid. It was suggested that the bile acid might be excreted by binding with Mg^<2+> into the bile.In conclusion, a new technique of ion chromatography may be useful for analysis of humoral ion levels whith can be applied to evaluate liver functions and diagnose as gastrointestinal diseases.