Cllnlcals study on the concentration by using a new ion chromatography of lonized trace elements and electrolytes in body flulds of patlents

新型离子色谱法测定患者体液中离子化微量元素和电解质浓度的临床研究

• 批准号: 07807115
• 负责人: UCHIYAMA Kazuhisa
• 金额: $ 1.28万
• 依托单位: Wakayama Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07807115/
• 关键词: ionized trace element; ionchromatography; 微量金属イオン; イオン化率

Clinicals study on the concentration by using a new ion chromatography of ionized trace elements and electrolytes in body fluids of patients with gastrointestinal disease.A new technique of ionchromatography was established for the simultaneous measurement of ionized trace elements (Fe^<2+>, Zn^<2+>, Mn^<2+>), electrolytes (Ca^<2+>, Mg^<2+>) and NH_<4+> in body fluids (serum, bile, pancreatic juice and urine). Among patients with gastrointestinal disease, Mg^<2+> concentration in serum was noticed to increase significantly in patients with hepatolithiasis (p<0.05).Although total concentration of zinc in serum of patients with liver dysfunction was lower than in normal subjects, serum Zn^<2+> concentration was higher (p<0.05). Therefore, the ionized ratio of zinc was found to elevate significantly. There was a negative correlationship between the concentration of Zn^<2+> and serum albumin level (r=0.62) or serum prealbumin level (r=0.73). The concentration of Ca^<2+> elevated significantly in bile of patients with calcium bilirubinate stone. The concentration of Mg^<2+> slightly elevated. It was confirmed that Ca^<2+> and Mg^<2+> showed a correlationship with total bile acid. In particular, Mg^<2+> was found to have a correlation with chenodeoxycholic acid. It was suggested that the bile acid might be excreted by binding with Mg^<2+> into the bile.In conclusion, a new technique of ion chromatography may be useful for analysis of humoral ion levels whith can be applied to evaluate liver functions and diagnose as gastrointestinal diseases.

应用离子色谱法测定胃肠疾病患者体液中离子化微量元素和电解质浓度的临床研究建立了一种同时测定离子化微量元素的离子色谱新技术体液（血清、胆汁、胰液和尿液）中的Fe^<2+>、Zn^<2+>、Mn^<2 +>、电解质（Ca^<2+>、Mg^<2+>）和NH_4+。在胃肠道疾病患者中，肝内胆管结石患者血清Mg^<2+>浓度显著升高（p<0.05），肝功能损害患者血清总锌浓度虽低于正常人，但血清Zn^<2+>浓度高于正常人（p<0.05）。因此，发现锌的离子化比率显著升高。Zn^<2+>浓度与血清白蛋白（r=0.62）和血清前白蛋白（r=0.73）呈负相关。胆红素钙结石患者胆汁中Ca^<2+>浓度明显升高. Mg^<2+>浓度略有升高。证实Ca^2+、Mg^2+与总胆汁酸呈正相关。特别是，Mg^<2+>与鹅去氧胆酸有相关性。结果表明，胆汁酸可能通过与Mg^<2+>结合进入胆汁而排出体外，离子色谱法是一种新的体液离子分析技术，可用于肝功能的评价和胃肠道疾病的诊断。

项目成果

内山和久: "胆嚢摘出術" 臨床外科. 51(11). 154-158 (1996)

内山和久：“胆囊切除术”临床外科 51(11) (1996)。

内山和久: "肝内結石症に合併する肝内胆管癌" 臨床外科. 52(2). 199-202 (1997)

Kazuhisa Uchiyama：“肝内胆管癌并发肝石症”临床外科 52(2) (1997)。

谷村弘: "日本臨床領域別症候群シリーズNO11." 日本臨床社 大阪, 3(49-51) (1996)

Hiroshi Tanimura：“日本临床区域综合症系列第 11 期”，日本临床出版有限公司，大阪，3(49-51) (1996)

谷村弘: "胆石症と栄養" 健帛社, 16(178-193) (1996)

谷村浩：“胆石症与营养” Kenpakusha，16(178-193) (1996)

内山和久: "肝内結石の治療" 外科治療. 74(4). 429-436 (1996)

Kazuhisa Uchiyama：“肝内结石的治疗”《外科治疗》74(4) (1996)。