Real time measurement of Hydroxylradical following ischemia-reperfusion injury model using rabbit liver.

The aim of this study was to investigate the generation of the hydroxyl radical(·OH)in hepatic tissue in vivo after ischemia/reperfusion(I/R)injury by using a microdialysis technique.Hepatic ischemia was induced in the male rabbit liver by clamping the hepatic artery and portal vein for 20minutes.Microdialysis probes were placed into the liver perfused with salicylate prior to，during，and after the period of clamping。In anesthetized male rabbits(n=45)，ischemia was produced by the selective vascular(Hepatic Artery And Portal Vein Without Caudal Lobe Branch)occlusion for 20min，the·OH-specific products of salycylate hydroxylation(2，3-and2，5-dihydroxybenzoic acid：DHBA)in the perfusion fluid from microdalysis into liver were measured by equipped with electrochemical HPLC 9hoafurwards。There was a significant increase(p&lt；0.05)in the intrahepatic·OH concentrations，compared to the non-ischemic control group，immediately after and seven hours after reperfusion.

The aim of this study was to investigate the generation of the hydroxyl radical(・OH) in hepatic tissue in vivo after ischemia/reperfusion(I/R) injury by using a microdialysis technique. Hepatic ischemia was induced in the male rabbit liver by clamping the hepatic artery and portal vein for 20 minutes. Microdialysis probes were placed into the liver perfused with salicylate prior to, during, and after the period of clamping. In anesthetized male rabbits(n=45), ischemia was produced by the selective vascular(hepatic artery and portal vein without caudal lobe branch) occlusion for 20 min, the・OH-specific products of salycylate hydroxylation(2, 3-and 2, 5-dihydroxybenzoic acid : DHBA) in the perfusion fluid from microdalysis into the liver were measured by HPLC equipped with electrochemical detector 9 hours afterwards. There was a significant increase(p<0.05) in the intrahepatic・OH concentrations, compared to the non-ischemic control group, immediately after and seven hours after reperfusion.