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THE STUDY FOR CELL RECOGNITION OF NON-SPECIFIC INTERACTION USING GENE EXPRESSION ANALYSIS

利用基因表达分析进行细胞非特异性相互作用识别的研究

基本信息

批准号：
07808100
负责人：
KISHIDA Akio
金额：
$ 1.34万
依托单位：
KAGOSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07808100/
关键词：
mRNA Polymer PCR HSP contact angle IL-1β

项目摘要

In this study, in order to investigate how cells recognize biomaterials, mRNA that was expressed in attached HeLa S3 cells on various substrates was evaluated. The expressed HSP 70A,70B,90 and 47 mRNA were then isolated and detected using the RT-PCR method. As a result, 70B,90 and 47 mRNA expressions varied with differences in the hydrophilicity-hydrophobicity of the substrates. The HSP mRNAs expressions were largely induced in HeLa S3 cells that had adhered onto hydrophilic surfaces. On the other hand, however, in the cells that had adhered onto hydrophobic surfaces, the HSP mRNAs expressions were low. In the non-adhered HeLa S3 cells the tendency of HSP 70B and HSP 47 mRNAs expressions were the same as in those of adhered cells, while there were no significant differences in the HSP 90 mRNA expression among the samples. It is clear that the study of HSP mRNAs expressions is very useful in the evaluation of cell-polymer interactions. The HSP 70B mRNA expression could be a very sensitive marker for the investigation of cell-polymer interactions. HeLa S3 cells that adhered onto hydrophilic surfaces suffered a heat-shock like stress-response, whereas the cells on the hydrophobic surfaces did not suffer any stimulation. We would emphasize that the investigation of mRNA expression is a powerful tool for studying cell-polymer interactions or the evaluation of the biocompatibility of biomaterials.

在本研究中，为了研究细胞如何识别生物材料，评估了在各种基质上附着的HeLa S3细胞中表达的mRNA。RT-PCR检测HSP 70 A、70 B、90和47的mRNA表达。结果，70 B，90和47 mRNA的表达随底物的亲水性-疏水性的差异而变化。HSPmRNA的表达在粘附到亲水表面的HeLa S3细胞中被大量诱导。然而，在另一方面，在已经粘附到疏水表面上的细胞中，HSP mRNA表达较低。在未贴壁的HeLa S3细胞中，HSP 70 B和HSP 47 mRNA的表达趋势与贴壁细胞相同，而HSP 90 mRNA的表达在不同样品间无明显差异。可见，研究HSP mRNA的表达对于评价细胞-多聚体相互作用是非常有用的。HSP 70 BmRNA的表达可作为研究细胞-聚合物相互作用的一个非常敏感的指标。粘附在亲水表面上的HeLa S3细胞遭受热休克样应激反应，而疏水表面上的细胞没有受到任何刺激。我们要强调的是，mRNA表达的研究是研究细胞-聚合物相互作用或评价生物材料生物相容性的有力工具。