Study on the Carcinogenesis and the Morphogenic Factors of Gallbladder Cancer.

胆囊癌发生及形态发生因素的研究。

• 批准号: 07671407
• 负责人: MIYAZAKI Kohji
• 金额: $ 1.34万
• 依托单位: Saga Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671407/
• 关键词: Gallbladder Cancer Cell Line; Growth Factors; Morphogenesis; Adhision Molecules; 間質細胞; 培養因子; 胆嚢癌; 増殖形態形成因子; in vitro; MRC5; 上皮-間質相互作用; epimorphin

To study the factors which regulate growth and morphology of gallbladder cancer, we have examined those of normal gallbladder cell and cell lines of gallbladder cancer in three-dimensioned collagen gels.Primary normal gallbladder cell formed cystic structure, which was enhanced by addition of EGF,HGF or epimorphin, whereas there were variations in the degree of the effects. On the contrary the subcultued cells had not formed cystic structure, but the addition of conditioned medium of fibroblasts or coculture with fibroblasts made them cystic. It was suggested that fibroblasts secrete some factor(s) which promotes regain of the cystic morphogenesis.On the other hand, gallbladder cancer cell lines rarely show cystic structure in collagen gels, and the above factors did not promote the cystic formation. Only GB-d1 formed cystic structure especially in floating collagen gel. EGF,HGF and conditioned medium of fibroblasts had the tendencies to stimulate the morphogenesis for branching or tubular formation.The above-mentioned differences between the normal and cancer cells supposed to be caused by the differences of intracellular signal transduction or those of the expressions of some adhesion molecules.

为探讨胆囊癌生长和形态的调控因素，我们在三维胶原凝胶中观察了正常胆囊细胞和胆囊癌细胞系的生长和形态，原代正常胆囊细胞形成囊状结构，加入EGF、HGF或epimorphin可促进囊状结构的形成，但作用程度不同。相反，继代培养的细胞尚未形成囊性结构，但加入成纤维细胞条件培养基或与成纤维细胞共培养使其囊性化。提示成纤维细胞分泌的某些因子促进了囊状形态的恢复，而胆囊癌细胞系很少在胶原凝胶中显示囊状结构，上述因子并不促进囊状形成。只有GB-d1形成囊状结构，尤其是在漂浮胶原凝胶中。EGF、HGF及成纤维细胞条件培养液有促进细胞分支或管状形成的倾向，正常细胞与癌细胞的上述差异可能是由于细胞内信号转导或某些粘附分子表达的差异所致。

项目成果

K.Miyazaki et.al.: "Hepatopancreatoduodenectomy for squamous and adenosquamous carcinoma of the gallbladder." Hepato-gastroenterol.42. 47-50 (1995)

K.Miyazaki 等人：“肝胰十二指肠切除术治疗胆囊鳞状癌和腺鳞癌。”

E.Sasatomi et.al.: "Spontaneous apoptosis in gallbladder carcinoma : Relationships with clinico-pathologic factors. expression of E-cadherin. bc1-2 protooncogene. and p53 oncosupressor gene." Cancer. 78. 2101-2110 (1996)

E.Sasatomi 等人：“胆囊癌的自发性细胞凋亡：与临床病理因素的关系。E-钙粘蛋白、bc1-2 原癌基因和 p53 抑癌基因的表达。”

K.Miyazaki et al.: "Hepatopancreato duodenectony for sguamous and a denosguamous catcinoma of the gallbladder" Hepato-gastroenterol.42. 47-50 (1995)

K.Miyazaki 等人：“胆囊鳞状细胞癌和腺鳞状细胞癌的肝胰十二指肠癌”肝胃肠病.42。

K.Miyazaki et.al.: "Hepatopancreatoduodenectomy for squamous and adenosquamous carcinoma of the gallbladder." Hepato-gastroenterol. 42. 47-50 (1995)

K.Miyazaki 等人：“肝胰十二指肠切除术治疗胆囊鳞状癌和腺鳞癌。”

向井伸介 他: "サイトカインによる胆嚢癌増殖形態の検討" 消化器癌の発生と進展. (発表予定). (1997)

Shinsuke Mukai 等人：“通过细胞因子检查胆囊癌的生长模式”胃肠癌的发生和进展（预定演示）。